Citation: LI Kang-Li, WANG Ling-Ling, BAO Ya-Jing, XIONG Bo, WANG Zhen-Yu. Analysis of Interaction between Pesticide and Biological Macromolecules Based on High Resolution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 218-223. doi: 10.11895/j.issn.0253-3820.150725 shu

Analysis of Interaction between Pesticide and Biological Macromolecules Based on High Resolution Mass Spectrometry

1.
The MOE Key Laboratory of Pesticide & Chemical Biology, Central China Normal University, Wuhan 430079, China

Corresponding author: XIONG Bo, WANG Zhen-Yu,
Received Date: 14 September 2015
Available Online: 5 November 2015
Fund Project: 本文系国家自然科学基金(Nos. 21205044, 21302060) (Nos. 21205044, 21302060)高校教育部基础研究经费(Nos. CCNU14A05005, CCNU15KFY004)的资助 (Nos. CCNU14A05005, CCNU15KFY004)

An analytical method for the determination of interaction between pesticide and biological macromolecules (bovine serum albumin (BSA) protein, superoxide dismutase (SOD) enzyme) was developed based on high resolution mass spectrometry. All results revealed that each BSA bonded with maximum five Parathion-methyls after 30 min, and it would not interact with emamectin benzoate. Moreover, each SOD after mixing with corresponding pesticides could bond with two dichlorvos (DDVPs) and one avermectin after 30 min. In addition, there is no interaction between SOD and thifluzamide. The interaction between SOD and avermectin trended stable after 30 min, and similar phenomenon was observed between SOD and DDVP after 20 min. There were certain obvious some differences in the pathway of interactions for BSA and SOD, both of which were bonded with the pesticides, based on time resolved determinations for their bonding process. Finally, we compared the proposed method with standard methods (fluorescence spectrometry, NBT kit method) and molecular docking results, and the reliability of the proposed method was supported. It owned preponderances in less sample consumption, high detection speed, and more capacities compared with traditional strategies. Therefore, the proposed mothed would own certain practical values in either researches and developments or safety evaluations for new pesticides.
- High resolution mass spectrometry,
- Molecular interactions,
- Pesticides,
- Bovine serum albumin,
- Superoxide dismutase
1. [1]
  1 Carvalho F P. Environ. Sci. Policy, 2006, 9(7-8): 685-692
2. [2]
  2 Carter W G, Tarhoni M H, Ray D E. J. Chromatogr. B, 2010, 878(17-18): 1312-1319
3. [3]
  3 Lockridge O. Adv. Molec.Toxicol., 2013, 7: 179-205
4. [4]
  4 Zhu Z F, Cheng J, Lu X L, Li X, Ge L Q, Fang J C, Wu J C. Pestic. Biochem. Physiol., 2014, 114: 97-101
5. [5]
  5 Mesomya W, Sutthivaiyakit P, Cuptapun Y, Hengsawadi D. Maejo Int. J.Sci. Tech., 2012, 6(3): 470-482
6. [6]
  6 Goodall S C, Spooner P, Middleton D, Howe P, Watts A. Biophys. J., 2002, 82(1 Part 2): 333a
7. [7]
  7 Thompson P M, Beck Moriah R, Campbell Sharon L. Method. Mol. Biol., 2015, 1278: 267-279
8. [8]
  8 WANG Qun, SHI Jing, ZHANG Huan, NI Jia-Zuan. Chinese J. Anal. Chem., 2005, 33(7): 909-912
9. [9]
  王群, 石晶, 张焕, 倪嘉缵. 分析化学, 2005, 33(7): 909-912
10. [10]
  9 XU Qian, DENG Dan-Dan, CAO Zhi-Juan, XIE Qiong, LIANG Jian-Ying, LU Jian-Zhong. Chinese J. Anal. Chem., 2010, 38(4): 483-487 徐倩, 邓丹丹, 曹志娟, 谢琼, 梁建英, 卢建忠. 分析化学, 2010, 38(4): 483-487
11. [11]
  10 Yue Y Y, Zhang Y H, Li Y, Zhu J H, Qin J, Chen X G. J. Lumin., 2008, 128(3): 513-520
12. [12]
  11 Kurahashi T, Miyazaki A, Suwan S, Isobe M. J. Am. Chem. Soc., 2001, 123(38): 9268-9278
13. [13]
  12 Liu L L, Ma Y J, Chen X Q, Xiong X, Shi S. Y. J. Chromatogr. B, 2012, 887: 55-60
14. [14]
  13 Mehta J V, Gajera S B, Patel M N. Spectrochim. Acta A, 2015, 136: 1881-1892
15. [15]
  14 ZHA li-Chun, HU Chun-Xiang, CAO Chuan-Wang, WANG Zhi-Ying, LIANG Chen. J. Northeast Forestry Uni., 2011, 5(39): 105-107 查黎春, 胡春祥, 曹传旺, 王志英, 梁臣. 东北林业大学学报, 2011, 5(39): 105-107
16. [16]
  15 Svedendahl M, Verre R, Käll M. Light Sci. Appl., 2014, 3(11): e220-e226
1. [1]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
2. [2]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
3. [3]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
4. [4]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
5. [5]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
6. [6]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
7. [7]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
8. [8]
  Hao Wu , Zhen Liu , Dachang Bai . ¹H NMR Spectrum of Amide Compounds. University Chemistry, 2024, 39(3): 231-238. doi: 10.3866/PKU.DXHX202309020
9. [9]
  Wen-Bing Hu . Systematic Introduction of Polymer Chain Structures. University Chemistry, 2025, 40(4): 15-19. doi: 10.3866/PKU.DXHX202401014
10. [10]
  Yuhui Yang , Jintian Luo , Biao Zuo . A Teaching Approach to Polymer Surface and Interface in Undergraduate Polymer Physics Courses. University Chemistry, 2025, 40(4): 126-130. doi: 10.12461/PKU.DXHX202408056
11. [11]
  Ping ZHANG , Chenchen ZHAO , Xiaoyun CUI , Bing XIE , Yihan LIU , Haiyu LIN , Jiale ZHANG , Yu'nan CHEN . Preparation and adsorption-photocatalytic performance of ZnAl@layered double oxides. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1965-1974. doi: 10.11862/CJIC.20240014
12. [12]
  Xueyu Lin , Ruiqi Wang , Wujie Dong , Fuqiang Huang . 高性能双金属氧化物负极的理性设计及储锂特性. Acta Physico-Chimica Sinica, 2025, 41(3): 2311005-. doi: 10.3866/PKU.WHXB202311005
13. [13]
  Laiying Zhang , Yinghuan Wu , Yazi Yu , Yecheng Xu , Haojie Zhang , Weitai Wu . Innovation and Practice of Polymer Chemistry Experiment Teaching for Non-Polymer Major Students of Chemistry: Taking the Synthesis, Solution Property, Optical Performance and Application of Thermo-Sensitive Polymers as an Example. University Chemistry, 2024, 39(4): 213-220. doi: 10.3866/PKU.DXHX202310126
14. [14]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
15. [15]
  Wenbing Hu , Jin Zhu . Flipped Classroom Approach in Teaching Professional English Reading and Writing to Polymer Graduates. University Chemistry, 2024, 39(6): 128-131. doi: 10.3866/PKU.DXHX202310015
16. [16]
  Pingsheng He , Haiyang Yang , Pingping Zhu . Philosophical Reflections in Polymer Physics Course: Emphasizing Reverse Thinking. University Chemistry, 2025, 40(4): 27-32. doi: 10.3866/PKU.DXHX202403029
17. [17]
  Rui Xu , Wei Li , Tianyi Li . Exploration of Teaching Reform in the Course of “Principles of Chemical Engineering” in the Polymer Materials and Engineering Major. University Chemistry, 2025, 40(4): 54-58. doi: 10.12461/PKU.DXHX202404081
18. [18]
  Chunyang Bao , Ruoxuan Miao , Yuhan Ding , Qingfu Ban , Yusheng Qin , Jie Liu , Zhirong Xin . The Comprehensive Experiment Design of Preparation of Depolymerizable Thermosetting Polymers. University Chemistry, 2025, 40(4): 59-65. doi: 10.12461/PKU.DXHX202405087
19. [19]
  Hujun Qian , Rui Shi , Guanglu Wu , Xuanbo Zhu . A Preliminary Study on the Development of a Virtual Simulation Platform for Polymer Physics Teaching and Its Teaching Practice. University Chemistry, 2025, 40(4): 147-153. doi: 10.12461/PKU.DXHX202409009
20. [20]
  Xiaofeng Zhu , Bingbing Xiao , Jiaxin Su , Shuai Wang , Qingran Zhang , Jun Wang . Transition Metal Oxides/Chalcogenides for Electrochemical Oxygen Reduction into Hydrogen Peroxides. Acta Physico-Chimica Sinica, 2024, 40(12): 2407005-. doi: 10.3866/PKU.WHXB202407005

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(377)
HTML views(30)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142