Citation: LI Kang-Li, WANG Ling-Ling, BAO Ya-Jing, XIONG Bo, WANG Zhen-Yu. Analysis of Interaction between Pesticide and Biological Macromolecules Based on High Resolution Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 218-223. doi: 10.11895/j.issn.0253-3820.150725 shu

Analysis of Interaction between Pesticide and Biological Macromolecules Based on High Resolution Mass Spectrometry

1.
The MOE Key Laboratory of Pesticide & Chemical Biology, Central China Normal University, Wuhan 430079, China

Corresponding author: XIONG Bo, WANG Zhen-Yu,
Received Date: 14 September 2015
Available Online: 5 November 2015
Fund Project: 本文系国家自然科学基金(Nos. 21205044, 21302060) (Nos. 21205044, 21302060)高校教育部基础研究经费(Nos. CCNU14A05005, CCNU15KFY004)的资助 (Nos. CCNU14A05005, CCNU15KFY004)

An analytical method for the determination of interaction between pesticide and biological macromolecules (bovine serum albumin (BSA) protein, superoxide dismutase (SOD) enzyme) was developed based on high resolution mass spectrometry. All results revealed that each BSA bonded with maximum five Parathion-methyls after 30 min, and it would not interact with emamectin benzoate. Moreover, each SOD after mixing with corresponding pesticides could bond with two dichlorvos (DDVPs) and one avermectin after 30 min. In addition, there is no interaction between SOD and thifluzamide. The interaction between SOD and avermectin trended stable after 30 min, and similar phenomenon was observed between SOD and DDVP after 20 min. There were certain obvious some differences in the pathway of interactions for BSA and SOD, both of which were bonded with the pesticides, based on time resolved determinations for their bonding process. Finally, we compared the proposed method with standard methods (fluorescence spectrometry, NBT kit method) and molecular docking results, and the reliability of the proposed method was supported. It owned preponderances in less sample consumption, high detection speed, and more capacities compared with traditional strategies. Therefore, the proposed mothed would own certain practical values in either researches and developments or safety evaluations for new pesticides.
- High resolution mass spectrometry,
- Molecular interactions,
- Pesticides,
- Bovine serum albumin,
- Superoxide dismutase
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沈阳化工大学材料科学与工程学院沈阳 110142