Citation: WANG Yan-Long, CHEN Jun-Hui, GAO Li-Yuan, WANG Shuai, ZHENG Xiao-Ling, SUN Cheng-Jun, WANG Xiao-Ru. Determination of Eight Typical Lipophilic Algae Toxins in Particles Suspended in Seawater by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 335-341. doi: 10.11895/j.issn.0253-3820.150714 shu

Determination of Eight Typical Lipophilic Algae Toxins in Particles Suspended in Seawater by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
¹ Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;
2.
² Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China

Corresponding author: CHEN Jun-Hui,
Received Date: 9 September 2015
Available Online: 20 December 2015
Fund Project: 本文系国家基金委-山东省联合基金项目(No.U1406403) (No.U1406403)中央级公益性科研院所基本科研业务费专项(No.2014T05) (No.2014T05)山东省自然科学基金(No.ZR2015PD003) (No.ZR2015PD003)

The particles suspended in seawater have great influence on pollutant migration and transformation in marine environment, while the lipophilic algae toxins enriched by the particles suspended in seawater will lead more serious toxicity to marine filter feeders. In this study, a new method was developed for the simultaneous determination of eight lipophilic algae toxins in suspended particles by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). After extracted with methanol by ultrasonic-assisted extraction, the sample was separated on an Acquity UPLC BEH C18 column (50 mm×2.1 mm, 1.7 μm) using gradient elution of acetonitrile and water containing 5 mmol/L ammonium acetate as eluent modifiers. The qualitative and quantitative analyses were carried out by electrospray ionization (ESI) tandem mass spectrometry in multiple reaction monitoring (MRM) mode. Under the optimal conditions, satisfactory precision (relative standard deviations (RSD≤14.1%), recoveries (83.8%-110.4%) and detection limits (2.9-103 pg/g) of the method were achieved. Good linearity (R²≥0.99) was also obtained for all studied analytes. Then, the method was applied to determine the amounts of the eight lipophilic marine toxins in authentic suspended particle samples collected from Qingdao near-shore area. Pectenotoxin 2 (PTX2) was detected in the samples from Shilaoren beach and No. 3 bathing beach with concentration ranges of 717 and 790 pg/g, respectively.
- Ultra performance liquid chromatography-tandem mass spectrometry,
- Lipophilic algae toxins,
- Seawater,
- Suspended particles,
- Pectenotoxin 2
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