Citation: FAN Xue-Mei, WANG Shu-Min, LI Zhe-Jian, ZHENG Xing-Wang. Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 342-347. doi: 10.11895/j.issn.0253-3820.150705 shu

Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor

1.
¹ College of Chemical Engineering and Modern Materials, Shangluo University, Shangluo 726000, China;
2.
² School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China

Corresponding author: FAN Xue-Mei,
Received Date: 6 September 2015
Available Online: 29 December 2015
Fund Project: 本文系国家自然科学基金(No.30970696) (No.30970696)陕西省科技统筹创新工程计划项目(2012KTDZ02-02) (2012KTDZ02-02)商洛市科技局计划项目(SK-2014-4) (SK-2014-4)陕西省尾矿资源综合利用重点实验室项目(14SKY-FWK06)资助。 (14SKY-FWK06)

Chitosan-Ru(bpy)₃²⁺-SiO₂ composite nanoparticles (CRuS NPs) were prepared by reverse microemulsion method, and based on the Nafion/MCNT composite membrane technology, CRuS NPs were effectively and steadily immobilize on the surface of a glassy carbon electrode to prepare the electrochemiluminescence sensor for uric acid determination. In 0.1 mol/L PBS (pH 7.4) buffer solution, when the actuation duration between uric acid and the modified electrode was 15 min, the electrochemiluminescence showed a good linear relationship to the negative logarithm of uric acid concentration in the range of 1.0 × 10^-10-1.0 × 10^-5 mol/L, the linear equation was I_ECL=-709.52-202.74lgC and the correlation coefficient was 0.9936 with a detection limit of 6.0 × 10^-12 mol/L. The ECL sensor exhibited excellent repeatability and stability, and the RSD for 11 times determination of 1.0 × 10^-8 mol/L uric acid was 2.9%. The recovery was 98.5%-103.5% in the determination of real Uric acid sample.
- Electrogenerated chemiluminescence,
- Chitosan-Ru(bpy)₃²⁺-SiO₂ nanoparticles,
- Uric acid
1. [1]
  1 Zuo R T, Zhou S, Zuo Y G, Deng Y W. Food Chem., 2015, 182(1): 242-245
2. [2]
  2 Remane D, Grunwald S, Hoeke H, Mueller A, Roeder S, Bergenb M, Wissenbach D K. J. Chromatogr. B, 2015, 998-999: 40-44
3. [3]
  3 Kumar V, Misr N, Paul J, Dhanawade B R, Varshney L. Polymer, 2014, 55(11): 2652-2660
4. [4]
  4 Wua D, Lua H F, Xie H, Wu J, Wang C M, Zhang Q L. Sens. Actuators, B, 2015, 221(31): 1433-1440
5. [5]
  5 Azmi N E, Ramli N I, Abdullah J, Hamid M A A, Sidek H, Rahman S A, Ariffin N, Yusof N A. Biosens. Bioelectron., 2015, 67(15): 129-133
6. [6]
  6 Bialokoz M M, Borowski P. J. Lumin., 2015, 160: 110-118
7. [7]
  7 Amjadi M, Manzoori J, Hallaj T. J. Lumin., 2014, 153: 73-78
8. [8]
  8 Yu J H, Wang S M, Ge L, Ge S G. Biosens. Bioelectron., 2011, 26(7): 3284-3289
9. [9]
  9 Yue H Y, Zhang H, Chang J, Gao X, Huang S,Yao L H, Lin X Y, Guo E J. Anal. Biochem., 2015, 488(1): 22-27
10. [10]
  10 FU Hai-Ying, WANG Jian-Xiu, DENG Liu. Chinese J. Anal. Chem., 2014, 42(3): 441-445付海莹, 王建秀, 邓留. 分析化学, 2014, 42(3): 441-445
11. [11]
  11 Chen D X, Wang Q, Jin J, Wu P, Wang H, Yu S Q, Zhang H, Cai C X. Anal. Chem., 2010, 82(6): 2448-2455
12. [12]
  12 Chu H H, Wei X H, Wu M S, Yan G L, Tu Y F. Sens. Actuators, B, 2012, 163(1): 247-252
13. [13]
  13 Tao Y W, Zhang X J, Wang J W, Wang XX, Yang N J. J. Electroanal. Chem., 2012, 674(1): 65-70
14. [14]
  14 Chu L,Zou G Z, Zhang X L. Mater. Sci. Eng., C, 2012, 32(8): 2169-2174
15. [15]
  15 Jia T T, Cai Z M, Chen X M, Lin Z J, Huang X L, Chen X, Chen G N. Biosens. Bioelectron., 2009, 25(1): 263-267
16. [16]
  16 Zhou L M, Huang J S, Yang L, Li L B, You T Y. Anal. Chim. Acta, 2014, 8 24(8): 57-63
17. [17]
  17 Dai H, Chi Y, Wu X, Wang Y, Wei M, Chen G. Biosens. Bioelectron., 2010, 25(6): 1414-1419
18. [18]
  18 Dang J, Guo Z H, Zheng X W. Anal. Chem., 2014, 86(18): 8943-8950
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Determination of Uric Acid Based on Chitosan/Ru(bpy)₃²⁺/Silica Nanoparticles Electrochemiluminescence Sensor