Citation: HAN Zhi-Zhong, WU Yue-Ting, ZHOU Ying, PAN Hai-Bo, CHEN Jing-Hua, LI Chun-Yan. A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 377-384. doi: 10.11895/j.issn.0253-3820.150694 shu

A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets

1.
¹ School of Pharmacy, Fujian Medical University, Fuzhou 350108, China;
2.
² College of Chemistry, Fuzhou University, Fuzhou 350108, China;
3.
³ Fujian Huishun Professional Health Evaluation Co. Ltd., Quanzhou 362000, China

Corresponding author: PAN Hai-Bo,
Received Date: 31 August 2015
Available Online: 6 January 2016
Fund Project: 本文系国家自然科学基金(No.21375017) (No.21375017)福建省自然科学基金(No.2015J05020) (No.2015J05020)福建省卫生计生委青年科研课题(No.2014-1-39) (No.2014-1-39)福建医科大学苗圃科研基金(No.2014MP008)资助 (No.2014MP008)

ZnO nanoparticles (ZnO NPs) were obtained by a direct precipitation method. With the as-prepared ZnO NPs as seeds, Au/ZnO heterostructure was synthesized by seed-mediated growth method without any surfactant, and the diameters of ZnO NPs and Au NPs were about 50 nm and 10 nm, respectively. Then ionic liquids (ILs), trihexyltetradecylphosphonium bis (trifluoromethylsulfonyl) imide ([P(C₆)₃C₁₄][Tf₂N]), and functionalized graphene (GN) were prepared under room temperature. The ILs as bridges could connect Au/ZnO heterostructure to form a new kind of graphene nanocomposite, Au/ZnO/GN. Then the penicillinase and hematein were immobilized on Au/ZnO/GN. And the biosensors based on penicillinase-hematein-Au/ZnO/GN (PH-AZG) were used for detecting penicillin G. In PBS buffer solution (pH 7.0), PH-AZG exhibited a detection range from 2.5×10^-14 to 3.3×10^-6 mol/L with a detection limit of 1.5×10^-14 mol/L (S/N≥ 3). Five PH-AZG electrodes were prepared with the same conditions, and the RSDs for their current response were less than 3.2%. Furthermore, the standard curves were linear in the range of 5×10^-14-5×10^-7 mol/L for milk. The average recoveries were 99.7%-101.4% with RSDs of 2.3%-3.5% (n=5). The method is sensitive and repeatable, and can be applied to the field of residue analysis about penicillins G with low concentration levels.
- Gold/zinc oxide heterostructure,
- Graphene,
- Ionic liquids,
- Penicillin biosensor
1. [1]
  1 Pennacchio A, Varriale A, Scala A, Marzullo V M, Staiano M, D'Auria S. Food Chem., 2016, 190: 381-385
2. [2]
  2 Piñero M Y, Bauza R, Arce L, Valcárcel M. Talanta, 2014, 119: 75-82
3. [3]
  3 Samanidou V, Evaggelopoulou E N. Pharm. Anal. Acta, 2015, 6(4): 1000e174
4. [4]
  4 Bailon-Perez A M, Garcia-Campana M I, del Olmo M, Iruela C-B C. J. Chromatogr. A, 2009, 1216(47): 8355-8361
5. [5]
  5 Kloth K., Rye-Johnsen M., Dither A., Dietrich R, Märtlbauer E, Niessner R, Seidel M. Analyst, 2009, 134(7): 1433-1439
6. [6]
  6 Chen Y, Wang Y, Liu L, Wu X, Xu L, Kuang H, Li A, Xu C. Nanoscale, 2015, 7(39): 16381-16388
7. [7]
  7 Yang H, Shan C, Li F, Han D, Zhang Q, Niu L. Chem. Commun., 2009, 45(26): 3880-3882
8. [8]
  8 XU Xin-Xin, HE Li-Jun, CAI Tian-Pei, YOU Li-Qin, XIANG Guo-Qiang, ZHAO Wen-Jie, JIANG Xiu-Ming. Chinese J. Anal. Chem., 2015, 43(6): 829-835许新新, 何丽君, 蔡天培, 游利琴, 向国强, 赵文杰, 江秀明. 分析化学, 2015, 43(6): 829-835
9. [9]
  9 Shan C S, Yang H F, Han D X, Zhang Q X, Ivaska A, Niu L. Biosens. Bioelectron., 2010, 25(6): 1504-1508
10. [10]
  10 Guo S J, Wen D, Zhai Y M, Dong S J, Wang E K. Biosens. Bioelectron., 2011, 26(8): 3475-3481
11. [11]
  11 Wang M L, Gao Y Q, Zhang J J, Zhao J W. Electrochim. Acta, 2015, 155: 236-243
12. [12]
  12 Liu N, Ma Z F. Biosens. Bioelectron., 2014, 51: 184-190
13. [13]
  13 Arya S K, Saha S, Ramirez-Vick J E, Gupta V, Bhansali S, Singh S P. Anal. Chim. Acta, 2012, 737: 1-21
14. [14]
  14 Congur G, Ates E S, Afal A, Unalan H E, Erdem A. J. Am. Ceram. Soc., 2015, 98(2): 663-668
15. [15]
  15 Hwa K-Y, Subramani B. Biosens. Bioelectron., 2014, 62: 127-133
16. [16]
  16 Wang X, Kong X G, Yu Y, Zhang H. J. Phys. Chem. C, 2007, 111(10): 3836-3841
17. [17]
  17 Daniel M C, Astruc D. Chem. Rev., 2004, 104(1): 293-346
18. [18]
  18 Wei Y Y, Li Y, Liu X Q, Xian Y Z, Shi G Y, Jin L T. Biosens. Bioelectron., 2010, 26(1): 275-278
19. [19]
  19 Hummers W S, Offeman R E. J. Am. Chem. Soc., 1958, 80(6): 1339-1339
20. [20]
  20 Choi B G, Park H, Park T J, Yang M H, Kim J S. ACS Nano, 2010, 4(5): 2910-2918
21. [21]
  21 Chen C C, Liu P, Lu C H. Chem. Eng. J., 2008, 144(3): 509-513
22. [22]
  22 Frens G. Nat. Phys. Sci., 1973, 241(105): 20-22
23. [23]
  23 Li D, Muller M B, Gilje S, Kaner R B. Nat. Nanotechnol., 2008, 3(2): 101-105
24. [24]
  24 Kim T Y, Lee H W, Kim J E, Suh K S. ACS Nano, 2010, 4(3): 1612-1618
25. [25]
  25 Li P, Wei Z, Wu T, Peng Q, Li Y. J. Am. Chem. Soc., 2011, 133(15): 5660-5663
26. [26]
  26 He C, Sasaki T, Shimizu Y, Koshizaki N. Appl. Surf. Sci., 2008, 254(7): 2196-2202
27. [27]
  27 Spanhel L, Anderson M A. J. Am. Chem. Soc., 1991, 113(8): 2826-2833
28. [28]
  28 Stred'ansky M, Pizzariello A, Stred'anská S, Miertuš S. Anal. Chim. Acta, 2000, 415(1-2): 151-157
29. [29]
  29 Liu J, Rinzler A G, Dai H J, Hafner J H, Bradley R K, Boul P J, Lu A, Iverson T, Shelimov K, Huffman C B, Rodriguez-M F, Shon Y S, Lee T R, Colbert D T, Smalley R E. Science, 1998, 280(5367): 1253-1256
30. [30]
  30 Yang C, Xu C, Wang X. Langmuir, 2012, 28(9): 4580-4585
31. [31]
  31 Chen B, Ma M, Su X. Anal. Chim. Acta, 2010, 674(1): 89-95
32. [32]
  32 Ibupoto Z H, Ali S M U, Khun K, Chey C O, Nur O, Willander M. Biosen., 2011, 1(4):153-163
1. [1]
  Chaolin Mi , Yuying Qin , Xinli Huang , Yijie Luo , Zhiwei Zhang , Chengxiang Wang , Yuanchang Shi , Longwei Yin , Rutao Wang . Galvanic Replacement Synthesis of Graphene Coupled Amorphous Antimony Nanoparticles for High-Performance Sodium-Ion Capacitor. Acta Physico-Chimica Sinica, 2024, 40(5): 2306011-0. doi: 10.3866/PKU.WHXB202306011
2. [2]
  Yameen Ahmed , Xiangxiang Feng , Yuanji Gao , Yang Ding , Caoyu Long , Mustafa Haider , Hengyue Li , Zhuan Li , Shicheng Huang , Makhsud I. Saidaminov , Junliang Yang . Interface Modification by Ionic Liquid for Efficient and Stable FAPbI₃ Perovskite Solar Cells. Acta Physico-Chimica Sinica, 2024, 40(6): 2303057-0. doi: 10.3866/PKU.WHXB202303057
3. [3]
  Anbang Du , Yuanfan Wang , Zhihong Wei , Dongxu Zhang , Li Li , Weiqing Yang , Qianlu Sun , Lili Zhao , Weigao Xu , Yuxi Tian . Photothermal Microscopy of Graphene Flakes with Different Thicknesses. Acta Physico-Chimica Sinica, 2024, 40(5): 2304027-0. doi: 10.3866/PKU.WHXB202304027
4. [4]
  Zhihuan XU , Qing KANG , Yuzhen LONG , Qian YUAN , Cidong LIU , Xin LI , Genghuai TANG , Yuqing LIAO . Effect of graphene oxide concentration on the electrochemical properties of reduced graphene oxide/ZnS. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1329-1336. doi: 10.11862/CJIC.20230447
5. [5]
  Tao Xu , Wei Sun , Tianci Kong , Jie Zhou , Yitai Qian . Stable Graphite Interface for Potassium Ion Battery Achieving Ultralong Cycling Performance. Acta Physico-Chimica Sinica, 2024, 40(2): 2303021-0. doi: 10.3866/PKU.WHXB202303021
6. [6]
  Qiang Zhang , Yuanbiao Huang , Rong Cao . Imidazolium-Based Materials for CO₂ Electroreduction. Acta Physico-Chimica Sinica, 2024, 40(4): 2306040-0. doi: 10.3866/PKU.WHXB202306040
7. [7]
  Ke Zhao , Zhen Liu , Luyao Liu , Changyuan Yu , Jingshun Pan , Xuguang Huang . Functionalized Reflective Structure Fiber-Optic Interferometric Sensor for Trace Detection of Lead Ions. Acta Physico-Chimica Sinica, 2024, 40(4): 2304029-0. doi: 10.3866/PKU.WHXB202304029
8. [8]
  Tianqi Bai , Kun Huang , Fachen Liu , Ruochen Shi , Wencai Ren , Songfeng Pei , Peng Gao , Zhongfan Liu . Nanoscale Mechanism of Microstructure-Dependent Thermal Diffusivity in Thick Graphene Sheets. Acta Physico-Chimica Sinica, 2025, 41(3): 2404024-0. doi: 10.3866/PKU.WHXB202404024
9. [9]
  Peiling Li , Qing Feng , Hongling Yuan , Qin Wang . Live Interview Recording about the Penicillin Family. University Chemistry, 2024, 39(9): 122-127. doi: 10.3866/PKU.DXHX202311022
10. [10]
  Jie XIE , Hongnan XU , Jianfeng LIAO , Ruoyu CHEN , Lin SUN , Zhong JIN . Nitrogen-doped 3D graphene-carbon nanotube network for efficient lithium storage. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1840-1849. doi: 10.11862/CJIC.20240216
11. [11]
  Tian TIAN , Meng ZHOU , Jiale WEI , Yize LIU , Yifan MO , Yuhan YE , Wenzhi JIA , Bin HE . Ru-doped Co₃O₄/reduced graphene oxide: Preparation and electrocatalytic oxygen evolution property. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 385-394. doi: 10.11862/CJIC.20240298
12. [12]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
13. [13]
  Yunting Shang , Yue Dai , Jianxin Zhang , Nan Zhu , Yan Su . Something about RGO (Reduced Graphene Oxide). University Chemistry, 2024, 39(9): 273-278. doi: 10.3866/PKU.DXHX202306050
14. [14]
  Wenjun Zheng . Application in Inorganic Synthesis of Ionic Liquids. University Chemistry, 2024, 39(8): 163-168. doi: 10.3866/PKU.DXHX202401020
15. [15]
  Junqing WEN , Ruoqi WANG , Jianmin ZHANG . Regulation of photocatalytic hydrogen production performance in GaN/ZnO heterojunction through doping with Li and Au. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 923-938. doi: 10.11862/CJIC.20240243
16. [16]
  Zhuo WANG , Junshan ZHANG , Shaoyan YANG , Lingyan ZHOU , Yedi LI , Yuanpei LAN . Preparation and photocatalytic performance of CeO₂-reduced graphene oxide by thermal decomposition. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1708-1718. doi: 10.11862/CJIC.20240067
17. [17]
  Zhenlin Zhou , Siyuan Chen , Yi Liu , Chengguo Hu , Faqiong Zhao . A New Program of Voltammetry Experiment Teaching Based on Laser-Scribed Graphene Electrode. University Chemistry, 2024, 39(2): 358-370. doi: 10.3866/PKU.DXHX202308049
18. [18]
  Jiahao Lu , Xin Ming , Yingjun Liu , Yuanyuan Hao , Peijuan Zhang , Songhan Shi , Yi Mao , Yue Yu , Shengying Cai , Zhen Xu , Chao Gao . High-Precision and Reliable Thermal Conductivity Measurement for Graphene Films Based on an Improved Steady-State Electric Heating Method. Acta Physico-Chimica Sinica, 2025, 41(5): 100045-0. doi: 10.1016/j.actphy.2025.100045
19. [19]
  Yu Guo , Zhiwei Huang , Yuqing Hu , Junzhe Li , Jie Xu . Recent Advances in Iron-based Heterostructure Anode Materials for Sodium Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(3): 2311015-0. doi: 10.3866/PKU.WHXB202311015
20. [20]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(469)
HTML views(30)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142