Citation: HAN Zhi-Zhong, WU Yue-Ting, ZHOU Ying, PAN Hai-Bo, CHEN Jing-Hua, LI Chun-Yan. A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(3): 377-384. doi: 10.11895/j.issn.0253-3820.150694 shu

A Low Detection Limit Penicillin Electrochemical Biosensor Based on Penicillinase-Hematein Au/ZnO/Single Graphene Nanosheets

1.
¹ School of Pharmacy, Fujian Medical University, Fuzhou 350108, China;
2.
² College of Chemistry, Fuzhou University, Fuzhou 350108, China;
3.
³ Fujian Huishun Professional Health Evaluation Co. Ltd., Quanzhou 362000, China

Corresponding author: PAN Hai-Bo,
Received Date: 31 August 2015
Available Online: 6 January 2016
Fund Project: 本文系国家自然科学基金(No.21375017) (No.21375017)福建省自然科学基金(No.2015J05020) (No.2015J05020)福建省卫生计生委青年科研课题(No.2014-1-39) (No.2014-1-39)福建医科大学苗圃科研基金(No.2014MP008)资助 (No.2014MP008)

ZnO nanoparticles (ZnO NPs) were obtained by a direct precipitation method. With the as-prepared ZnO NPs as seeds, Au/ZnO heterostructure was synthesized by seed-mediated growth method without any surfactant, and the diameters of ZnO NPs and Au NPs were about 50 nm and 10 nm, respectively. Then ionic liquids (ILs), trihexyltetradecylphosphonium bis (trifluoromethylsulfonyl) imide ([P(C₆)₃C₁₄][Tf₂N]), and functionalized graphene (GN) were prepared under room temperature. The ILs as bridges could connect Au/ZnO heterostructure to form a new kind of graphene nanocomposite, Au/ZnO/GN. Then the penicillinase and hematein were immobilized on Au/ZnO/GN. And the biosensors based on penicillinase-hematein-Au/ZnO/GN (PH-AZG) were used for detecting penicillin G. In PBS buffer solution (pH 7.0), PH-AZG exhibited a detection range from 2.5×10^-14 to 3.3×10^-6 mol/L with a detection limit of 1.5×10^-14 mol/L (S/N≥ 3). Five PH-AZG electrodes were prepared with the same conditions, and the RSDs for their current response were less than 3.2%. Furthermore, the standard curves were linear in the range of 5×10^-14-5×10^-7 mol/L for milk. The average recoveries were 99.7%-101.4% with RSDs of 2.3%-3.5% (n=5). The method is sensitive and repeatable, and can be applied to the field of residue analysis about penicillins G with low concentration levels.
- Gold/zinc oxide heterostructure,
- Graphene,
- Ionic liquids,
- Penicillin biosensor
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