Citation: LIN Chun-Hua, LIU De-Yong, FAN Nai-Li, XIA Jian-Hui, LIAO Wei-Lin. Determination of Main Composition of Fatty Acid Monoglyceride in Monoglyceride Emulsifier Using Ultra Performance Convergence Chromatography-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 281-288. doi: 10.11895/j.issn.0253-3820.150558 shu

Determination of Main Composition of Fatty Acid Monoglyceride in Monoglyceride Emulsifier Using Ultra Performance Convergence Chromatography-Mass Spectrometry

1.
¹ National Monosaccharide Chemical Synthesis Engineering Research Center, Jiangxi Normal University, Nanchang 330027, China;
2.
² Chemistry and Chemical Engineering Department, Jiangxi Normal University, Nanchang 330027, China

Corresponding author: XIA Jian-Hui,
Received Date: 14 July 2015
Available Online: 21 October 2015
Fund Project: 本文系国家科技支撑计划项目(Nos.2012BAE07B00,2014BAE13B02)资助 (Nos.2012BAE07B00,2014BAE13B02)

A fast analytical method was developed for the determination of main composition of fatty acid monoglycerides, including monopalmitin, monostearin, monoolein and monolinolein, in monoglycerede emulsifiers by ultra-performance convergence chromatography-mass spectrometry. Their contents were compared in the 3 emulsifiers from different sources. The sample was directly dissolved with n-hexane/isopropanol (7:3, /V) The chromatographic separation was performed on the ACQUITY UPC² BEH 2-EP column (2.1 mm×100 mm, 1.7 μm) using the mobile phases of carbon dioxide and methanol/acetonitrile (1:1, V/V) solution with gradient elution. The separated compounds were detected by MS detector in positive electrospray ionization (ESI⁺) and quantified by external standard method. The results showed that the calibration curves of monopalmitin,monostearin,monoolein and monolinolein were linear in the range of 0.20-50 mg/L, 0.20-50 mg/L, 0.25-62.5 mg/L and 0.20-50 mg/L, respectively, with correlation coefficients not less than 0.9983. The limits of quantification (S/N≥10) of the four fatty acid monoglycerides were 0.018-0.046 mg/L. The average recoveries for the four monoglycerides at three spiked levels were 88.0%-110.5% with relative standard deviations of 1.1%-4.1%. The proposed method showed high performance, good selectivity and fast analysis for the underivatized monoglyceride samples. It would provide a new chromatographic technology for the content analysis of monoglycerides in the emulsifier.
- Ultra-performance convergence chromatography-mass spectrometry,
- Fatty acid monoglyceride,
- Emulsifier,
- Underivatization
1. [1]
  1 LI Yan, LIU Jun-Hai. Cereals & Oils, 2011, (3): 7-11
2. [2]
  李燕, 刘军海. 粮食与油脂, 2011(3): 7-11
3. [3]
  2 SHI Hong, GUO Hong. China Oils and Fats, 2000, 25(4): 38-41 时宏, 郭洪. 中国油脂, 2000, 25(4): 38-41
4. [4]
  3 NI Yong-Quan. The Third Member of China Food Additive Association Congress and the Ninth China International Exhibition of Food Additives and Ingredients of Academic Papers, 2005: 3 倪永全. 中国食品添加剂协会第三届会员代表大会暨第九届中国国际食品添加剂和配料展览会学术论文集, 2005: 3
5. [5]
  4 LIU Yan-Feng, HUANG Hui-Hua. Modern Food Science and Technology, 2012, 28(1): 86-90 刘艳丰, 黄惠华. 现代食品科技, 2012, 28(1): 86-90
6. [6]
  5 MOU Ying, CUI Hai-Ping, YANG Tian-Kui. China Food Additives, 2012, S1: 88-92 牟英, 崔海萍, 杨天奎. 中国食品添加剂, 2012, S1: 88-92
7. [7]
  6 YI Xia, FAN Tie. China Oils and Fats, 2008, 33(9): 73-75 栾霞, 樊铁. 中国油脂, 2008, 33(9): 73-75
8. [8]
  7 MENG Xiang-He, ZHANG Yin-Jun, MAO Zhong-Gui. China Oils and Fats, 2004, 29(1): 44-46 孟祥河, 章银军, 毛忠贵. 中国油脂, 2004, 29(1): 44-46
9. [9]
  8 ZHANG Bo, SI Zhi-Kun. Shandong Chem Industry, 2003, 32(6): 27-28 张博, 司芝坤. 山东化工, 2003, 32(6): 27-28
10. [10]
  9 FENG Feng-Qin, YANG Hong-Jie. Chinese Food Science, 2005, 5(1): 62-66 冯凤琴, 杨宏杰. 中国食品学报, 2005, 5(1): 62-66
11. [11]
  10 WANG Peng, SUN Yang, LIU Zhe-Yi, FU Yu-Fei, PAN Zai-Fa, WANG Li-Li. Chinese J. Anal. Chem., 2011, 39(9): 1427-1431 王鹏, 孙杨, 刘哲益, 傅宇飞, 潘再法, 王丽丽. 分析化学, 2011, 39(9): 1427-1431
12. [12]
  11 WAGN Li-Li, WAGN Yong, HU Chang-Ying, ZHANG Yun. China Oils and Fats, 2011, 36(7): 75-79 王丽丽, 汪勇, 胡长鹰, 张云. 中国油脂, 2011, 36(7): 75-79
13. [13]
  12 MA Yu-Song, JIA Hai-Tao, YAO Chun-Yi, GUO Chun-Hai. Journal of Instrumental Analysis, 2013, 32(8): 1012-1015 马育松,贾海涛,姚春毅,郭春海. 分析测试学报, 2013, 32(8): 1012-1015
14. [14]
  13 Takano S, Kondoh Y. J. Am. Oil Chem. Soc., 1987, 64(7): 1001-1003
15. [15]
  14 Di Nicola G, Pacetti M, Polonara F, Santori G, Stryjekb R. J. Chromatogr. A, 2008, 1190(1): 120-126
16. [16]
  15 LIU Liu, LU Zong-Feng, LI Li-Jun, LAI Ying-Biao. Physical Testing and Chemical Analysis Part B: Chemical Analysis, 2011, 47(12): 1468-1469 刘柳, 陆宗峰, 李利军, 赖映标. 理化检验(化学分册), 2011, 47(12): 1468-1469
17. [17]
  16 Fedosova S N, Fernandesb N A, Firdausa M Y. J. Chromatogr. A, 2014, 1326: 56-62
18. [18]
  17 YANG Jing, HU Dao-Hua. Science and Technology of Food Industry, 2004, 25(7): 127-128 杨菁, 胡道华. 食品工业科技, 2004, 25(7): 127-128
19. [19]
  18 Freedman B, Pryde E H, Kwolek W F. J. Am. Oil Chem. Soc., 1984, 61: 1215-1220
20. [20]
  19 Fagan P, Wijesundera C, Watkins P. J. Chromatogr. A, 2004, 1054(1): 251-259
21. [21]
  20 Csernica S N, Hsu J T. Energy Fuels, 2010, 24: 6131-6141
22. [22]
  21 LIN Chun-Hua, FAN Nai-Li, RUI Pei-Xin, XIA Jian-Hui, LIAO Wei-Lin, YANG Shao-Ming. Chinese J. Anal. Chem., 2015, 43(1): 75-80 林春花, 范乃立, 芮培欣, 夏剑辉, 廖维林, 杨绍明. 分析化学, 2015, 43(1): 75-80
23. [23]
  22 Bamba T, Lee J W, Matsubara A, Fukusaki E. J. Chromatogr. A, 2012, 1250: 212-219
1. [1]
  Yanhui Zhong , Ran Wang , Zian Lin . Analysis of Halogenated Quinone Compounds in Environmental Water by Dispersive Solid-Phase Extraction with Liquid Chromatography-Triple Quadrupole Mass Spectrometry. University Chemistry, 2024, 39(11): 296-303. doi: 10.12461/PKU.DXHX202402017
2. [2]
  Yang WANG , Xiaoqin ZHENG , Yang LIU , Kai ZHANG , Jiahui KOU , Linbing SUN . Mn single-atom catalysts based on confined space: Fabrication and the electrocatalytic oxygen evolution reaction performance. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2175-2185. doi: 10.11862/CJIC.20240165
3. [3]
  Wen YANG , Didi WANG , Ziyi HUANG , Yaping ZHOU , Yanyan FENG . La promoted hydrotalcite derived Ni-based catalysts: In situ preparation and CO₂ methanation performance. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 561-570. doi: 10.11862/CJIC.20230276
4. [4]
  Lina Guo , Ruizhe Li , Chuang Sun , Xiaoli Luo , Yiqiu Shi , Hong Yuan , Shuxin Ouyang , Tierui Zhang . 层状双金属氢氧化物的层间阴离子对衍生的Ni-Al₂O₃催化剂光热催化CO₂甲烷化反应的影响. Acta Physico-Chimica Sinica, 2025, 41(1): 2309002-. doi: 10.3866/PKU.WHXB202309002
5. [5]
  Zunxiang Zeng , Yuling Hu , Yufei Hu , Hua Xiao . Analysis of Plant Essential Oils by Supercritical CO₂Extraction with Gas Chromatography-Mass Spectrometry: An Instrumental Analysis Comprehensive Experiment Teaching Reform. University Chemistry, 2024, 39(3): 274-282. doi: 10.3866/PKU.DXHX202309069
6. [6]
  Yikai Wang , Xiaolin Jiang , Haoming Song , Nan Wei , Yifan Wang , Xinjun Xu , Cuihong Li , Hao Lu , Yahui Liu , Zhishan Bo . 氰基修饰的苝二酰亚胺衍生物作为膜厚不敏感型阴极界面材料用于高效有机太阳能电池. Acta Physico-Chimica Sinica, 2025, 41(3): 2406007-. doi: 10.3866/PKU.WHXB202406007
7. [7]
  Asif Hassan Raza , Shumail Farhan , Zhixian Yu , Yan Wu . 用于高效制氢的双S型ZnS/ZnO/CdS异质结构光催化剂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406020-. doi: 10.3866/PKU.WHXB202406020
8. [8]
  Qingqing SHEN , Xiangbowen DU , Kaicheng QIAN , Zhikang JIN , Zheng FANG , Tong WEI , Renhong LI . Self-supporting Cu/α-FeOOH/foam nickel composite catalyst for efficient hydrogen production by coupling methanol oxidation and water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1953-1964. doi: 10.11862/CJIC.20240028
9. [9]
  Shuang Yang , Qun Wang , Caiqin Miao , Ziqi Geng , Xinran Li , Yang Li , Xiaohong Wu . Ideological and Political Education Design for Research-Oriented Experimental Course of Highly Efficient Hydrogen Production from Water Electrolysis in Aerospace Perspective. University Chemistry, 2024, 39(11): 269-277. doi: 10.12461/PKU.DXHX202403044
10. [10]
  Ruolin CHENG , Haoran WANG , Jing REN , Yingying MA , Huagen LIANG . Efficient photocatalytic CO₂ cycloaddition over W₁₈O₄₉/NH₂-UiO-66 composite catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 523-532. doi: 10.11862/CJIC.20230349
11. [11]
  Qiangqiang SUN , Pengcheng ZHAO , Ruoyu WU , Baoyue CAO . Multistage microporous bifunctional catalyst constructed by P-doped nickel-based sulfide ultra-thin nanosheets for energy-efficient hydrogen production from water electrolysis. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1151-1161. doi: 10.11862/CJIC.20230454
12. [12]
  Wei Zhong , Dan Zheng , Yuanxin Ou , Aiyun Meng , Yaorong Su . K原子掺杂高度面间结晶的g-C₃N₄光催化剂及其高效H₂O₂光合成. Acta Physico-Chimica Sinica, 2024, 40(11): 2406005-. doi: 10.3866/PKU.WHXB202406005
13. [13]
  Pingwei Wu . Application of Diamond Software in Simplex Teaching. University Chemistry, 2024, 39(3): 118-121. doi: 10.3866/PKU.DXHX202311043
14. [14]
  Jingzhao Cheng , Shiyu Gao , Bei Cheng , Kai Yang , Wang Wang , Shaowen Cao . 4-氨基-1H-咪唑-5-甲腈修饰供体-受体型氮化碳光催化剂的构建及其高效光催化产氢研究. Acta Physico-Chimica Sinica, 2024, 40(11): 2406026-. doi: 10.3866/PKU.WHXB202406026
15. [15]
  Rui Li , Huan Liu , Yinan Jiao , Shengjian Qin , Jie Meng , Jiayu Song , Rongrong Yan , Hang Su , Hengbin Chen , Zixuan Shang , Jinjin Zhao . 卤化物钙钛矿的单双向离子迁移. Acta Physico-Chimica Sinica, 2024, 40(11): 2311011-. doi: 10.3866/PKU.WHXB202311011
16. [16]
  Ling Liu , Haibin Wang , Genrong Qiang . Curriculum Ideological and Political Design for the Comprehensive Preparation Experiment of Ethyl Benzoate Synthesized from Benzyl Alcohol. University Chemistry, 2024, 39(2): 94-98. doi: 10.3866/PKU.DXHX202304080
17. [17]
  Weina Wang , Fengyi Liu , Wenliang Wang . “Extracting Commonality, Delving into Typicals, Deriving Individuality”: Constructing a Knowledge Graph of Crystal Structures. University Chemistry, 2024, 39(3): 36-42. doi: 10.3866/PKU.DXHX202308029
18. [18]
  Shuying Zhu , Shuting Wu , Ou Zheng . Improvement and Expansion of the Experiment for Determining the Rate Constant of the Saponification Reaction of Ethyl Acetate. University Chemistry, 2024, 39(4): 107-113. doi: 10.3866/PKU.DXHX202310117
19. [19]
  Jianfeng Yan , Yating Xiao , Xin Zuo , Caixia Lin , Yaofeng Yuan . Comprehensive Chemistry Experimental Design of Ferrocenylphenyl Derivatives. University Chemistry, 2024, 39(4): 329-337. doi: 10.3866/PKU.DXHX202310005
20. [20]
  Ruitong Zhang , Zhiqiang Zeng , Xiaoguang Zhang . Improvement of Ethyl Acetate Saponification Reaction and Iodine Clock Reaction Experiments. University Chemistry, 2024, 39(8): 197-203. doi: 10.3866/PKU.DXHX202312004

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(422)
HTML views(65)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142