Citation: JIANG Li-Ying, XIAO Xiao-Nan, ZHOU Peng-Lei, ZHANG Pei, YAN Yan-Xia, JIANG Su-Xia, CHEN Qing-Hua. An Aptamer-based Fluorescence Biosensor for Insulin Detection Based on Graphene Oxide[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 310-314. doi: 10.11895/j.issn.0253-3820.150534 shu

An Aptamer-based Fluorescence Biosensor for Insulin Detection Based on Graphene Oxide

1.
Zhengzhou University of Light Industry, Institute of Electrical and Information Engineering, Zhengzhou 450002 China

Corresponding author: JIANG Li-Ying,
Received Date: 3 July 2015
Available Online: 13 November 2015
Fund Project: 本文系国家自然科学基金(No. 61002007) (No. 61002007)河南省科技创新人才计划(No. 124100510001) (No. 124100510001)郑州轻工业学院2014年度研究生科技创新基金资助项目(No. 2014010) (No. 2014010)郑州轻工业学院博士科研基金资助项目(No. 2014BSJJ041) (No. 2014BSJJ041)

By using fluorophore (FAM) labeled aptamers as recognition elements and graphene oxide as a quencher, a highly selective and sensitive sensor was constructed for the fast and accurate detection of insulin. After the aptamer binding with graphene oxide, fluorescence will be quenched and fluorescence intensity disappeared; after addition of insulin, the solution fluorescence was restored. Based on the fact, we established a method for the determination of insulin concentration by measuring the fluorescence intensity. The results showed that the concentration of insulin in the range of 5×10^-8-1×10^-5 mol/L had a good linear relationship with the fluorescence intensity. The detection limit was 10 nmol/L. This method of detecting insulin had obvious advantages of fast detection speed, high selectivity and low detection limit.
- Fluorophore,
- Aptamer,
- Graphene oxide,
- Fluorescence aptamer biosensor,
- Insulin
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