Citation: LIU Ping, HAN Xiao-Yue, YU Fa-Biao, CHEN Ling-Xin. A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells[J]. Chinese Journal of Analytical Chemistry, ;2015, 43(12): 1829-1836. doi: 10.11895/j.issn.0253-3820.150445 shu

A Near-Infrared Fluorescent Probe for Detection of Nitroxyl in Living Cells

LIU Ping ¹ ,
HAN Xiao-Yue ^1,2 ,
YU Fa-Biao ^1,, ,
CHEN Ling-Xin ^1,,

1.
¹ Key Laboratory of Coastal Environmental Processes and Ecological Remediation, The Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;

Corresponding author: YU Fa-Biao, CHEN Ling-Xin,
Received Date: 28 May 2015
Available Online: 21 August 2015
Fund Project: 本文系国家自然科学基金资助项目(Nos.21405172,21575159,21275158) (Nos.21405172,21575159,21275158)中国科学院青年创新促进会(Grant2015170) (Grant2015170)

Nitroxyl(HNO), the one-electron reduced and protonated congener of nitric oxide(NO), has been demonstrated with excellent bio-pharmacological effects in cardiovascular disorder treatment, which is distinctive from that of NO. The high reactivity of HNO challenges the accurate detection. To resolve this problem, a near-infrared(NIR) metal-free fluorescent probe, ER-JN, was developed for the detection of intracellular HNO concentration in simulated physiological conditions and living cells. The probe was consisted of two moieties, the A BF2-chelated tetraarylazadipyrromethane fluorophore(aza-BODIPY) and the HNO recognition unit, diphenylphosphinobenzoyl group. The probe was purified by column chromatography on silica eluting with CH₂Cl₂ to give the product as a green solid with a yield of 28%. Our probe exhibited high sensitivity, good selectivity, and low cytotoxic effect, and could be applied to the fluorescent bio-imaging of HNO in simulated physiological conditions. When detected HNO, quantum yield increased from 0.01 to 0.35. The linear range located at 0-50μmol/L. The detection limit was 0.03μmol/L(S/N=3). With confocal laser scanning microscope imaging analysis, the probe could detect HNO concentration changes in living cells. Furthermore, the results of flow cytometry confirmed that our probe could be employed for the qualitative and quantitative detection of intracellular HNO concentration level. In this work, we found that probe ER-JN could not only detect HNO in aqueous and in living cells, but also targets endoplasmic reticulum. We anticipated that ER-JN would provide experimental bases in studying physiological and pathological functions of HNO in cells, in vitro and in vivo.
- Fluorescent probe,
- Nitroxyl,
- Cell analysis,
- Near-infrared bioimaging
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