Citation: WEI Lin-Hong, LIU Lin, LÜ Hong-Ying, TENG Zhen-Yuan, KANG Hui-Min, WANG Cheng-Yin, HU Xiao-Ya. An Impedance Immunosensor for Detection of Uniconazole Based on Screen Printed Electrode[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(2): 258-264. doi: 10.11895/j.issn.0253-3820.150391 shu

An Impedance Immunosensor for Detection of Uniconazole Based on Screen Printed Electrode

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¹ College of Chemistry and Chemical Engineering, Jiangsu Key Laboratory of Environmental Engineering and Materials, Yangzhou University, Yangzhou 225002, China;
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² College of Biological and Chemical Engineering, Yangzhou Vocational University, Yangzhou 225009, China

Corresponding author: WANG Cheng-Yin,
Received Date: 12 May 2015
Available Online: 18 November 2015
Fund Project: 本文系国家自然科学基金(No. 21375116)江苏省高等职业院校国内高级访问学者计划资助项目(No.2014FX092)江苏省环境材料与环境工程重点实验室2014年度开放基金项目(No.K14019)资助项目 (No. 21375116)江苏省高等职业院校国内高级访问学者计划资助项目(No.2014FX092)江苏省环境材料与环境工程重点实验室2014年度开放基金项目(No.K14019)

Polyaniline/chitosan composite membrane was modified on the surface of a screen printed carbon electrode by cyclic voltammetry (CV), and then anti-uniconazole antibodies were immobilized on the electrode based on electrostatic attraction. The impedance immunosensor for the determination of uniconazole was thus constructed. CV and electrochemical impedance spectroscopy (EIS) were used to characterize the electrochemical properties of immunosensor. Several factors affecting the immunoreaction were investigated, including the pH of phosphate buffer saline (PBS), incubation temperature and time. The performance of the proposed immunosensor was investigated under the optimized conditions. The immunosensor exhibited advantages of high accuracy, high sensitivity and specificity, good reproducibility and stability. The immunosensor could detect uniconazole in a linear range of 0.01-100 mg/L (R=0.998), and the detection limit was 8 μg/L (3σ). The average recoveries were 98.5%-104.6% and the relative standard deviations were 3.3-4.3% at the spiked level of 0.05, 0.10 and 1.0 mg/kg in cabbage, and the detection results were consistent with the results obtained by gas chromatography.
- Immunosensor,
- Electrochemical impedance spectroscopy,
- Uniconazole,
- Screen printed electrode
1. [1]
  1 XU Xin-Juan, HUANG Zhong-Wen, SUN Hai-Yan. Journal of Henan Institute of Science and Technology, 2007, 35(4): 47-49 徐新娟, 黄中文, 孙海燕. 河南科技学院学报(自然科学版), 2007, 35(4): 47-49
2. [2]
  2 YU Hong, LU Yi, WANG Jing-Jing, YANG Tao, Zhang Huang-Tao. Xinjiang Agricultural Sciences, 2011, 48(1): 187-193 于红, 鹿毅, 王静静, 杨涛, 张煌涛. 新疆农业科学, 2011, 48(1): 187-193
3. [3]
  3 National Food Safety Standard-Maximun Residue limits for Pesticides in Food. National Standards of the People's Republic of China. GB 2763-2012 食品安全国家标准-食品中农药最大残留限量. 中华人民共和国国家标准. GB 2763-2012
4. [4]
  4 JIN Qi-Yan, YUAN Xu, WANG Ping. Modern Scientific Instruments, 2014, 5(10): 113-115 晋齐艳, 袁旭, 王萍. 现代科学仪器, 2014, 5(10): 113-115
5. [5]
  5 ZHANG Feng-Zu, FAN Su-Fang, GU Ke-Jia, DENG Kai-Lin, PAN Can-Ping. Bull. Environ. Contam. Toxicol., 2013, 90(4): 499-503
6. [6]
  6 XU Guo-Feng, NIE Ji-Yun, LI Jing, LI Hai-Fei, YAN Zhen. Pesticide, 2014, 53(10): 736-738 徐国锋, 聂继云, 李静, 李海飞, 闫震. 农药, 2014, 53(10): 736-738
7. [7]
  7 WANG Jing-Jing, SU Min, GONG Zhi-Guo, SHANG De-Jun. Chemical Analysis and Meterage, 2014, 23(5): 135-138 王静静, 苏敏, 巩志国, 李世雨, 尚德军. 化学分析计量, 2014, 23(5): 135-138
8. [8]
  8 HUANG He-He, ZHANG Jin, XU Dun-Ming, ZHOU Hao, LUO Jia, LÜ Mei-Ling, CHEN Shu-Bin,WANG Lian-Zhu. Chinese Journal of Chromatography, 2014, 32(7): 707-716 黄何何, 张缙, 徐敦明, 周昱, 罗佳, 吕美玲, 陈树宾, 王连珠. 色谱, 2014, 32(7): 707-716
9. [9]
  9 ZHANG Liang, WANG Qun-Zhen, WANG-Ning, LIU Shu-Zhao. Chinese J. Anal. Chem., 2012, 40(11): 1730-1734 张良, 王泉振, 王宁, 刘曙照. 分析化学, 2012, 40(11): 1730-1734
10. [10]
  10 YANG Xin. Electrochemical Biosensors for Detection of Veterinary Drug and Pesticide Residues Based on Screen-printed Carbon Electrodes. Ningbo: Ningbo University.2010 杨欣. 基于丝网印刷电极的电化学生物传感器及其在兽药和农药残留检测中的应用. 宁波: 宁波大学, 2010
11. [11]
  11 Zahra T, Alireza K, Mohammad M A. Microchim. Acta, 2014, 181(9-10): 865-891
12. [12]
  12 ZHANG Jin-Guo, KANG Tian-Fang, XUE Rui, SUN Xue. Chinese J. Anal. Chem., 2013, 41(9): 1353-1358 张金果, 康天放, 薛瑞, 孙雪. 分析化学, 2013, 41(9): 1353-1358
13. [13]
  13 Truong L T N, Chikae M, Ukita Y, Takamura Y. Talanta, 2011, 85(5): 2576-2580
14. [14]
  14 Hervas M, Lopez M A, Escarpa A. Biosens. Bioelectron., 2010, 25(7): 1755-1760
15. [15]
  15 Raju K, Marshal D. Biosens. Bioelectron., 2009, 24(6): 1700-1705
16. [16]
  16 XUE Huai-Guo, SHEN Zhi-Quan, ZHANG Yi-Feng, LI Yong-Fang. Chemistry, 2001, 64(7): 402-406 薛怀国, 沈之荃, 张一烽, 李永舫. 化学通报, 2001, 64(7): 402-406
17. [17]
  17 LIU Xue-Ping, ZHANG Yi-Fang, WU Hui-Wang, SHEN Guo-Li, YU Ru-Qin. Chinese J. Anal. Chem., 2012, 40(8): 1147-1152 刘雪平, 张一芳, 吴会旺, 沈国励, 俞汝勤. 分析化学, 2012, 40(8): 1147-1152
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