Citation: DENG Huan, SUN Mi, WEI Xiao-Ping, LI Hong-Zeng, LI Jian-Ping. Electrochemical Lead Ion Aptasensor Based on G-Quadruplex and Gate-controlled Signal Amplification[J]. Chinese Journal of Analytical Chemistry, ;2016, 44(6): 888-892. doi: 10.11895/j.issn.0253-3820.150314 shu

Electrochemical Lead Ion Aptasensor Based on G-Quadruplex and Gate-controlled Signal Amplification

Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China

Received Date: 18 April 2015
Revised Date: 7 January 2016

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21375031) and the Natural Science Foundation of Guangxi Province of China (No.2015GXNSFFA139005)

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A new strategy for constructing electrochemical aptasensor for lead ion (Pb²⁺) analysis with extremely high sensitivity was proposed based on the formation of G-quadruplex (G4) when Pb²⁺ reacting with G-rich aptamers, and the gate-controlled effect was achieved through the controllable channels of probe. SH-β-cyclodextrins were firstly self-assembled on the surface of gold electrode to form an ordered monomolecular layer containing interspaces among β-CD units. When the G-rich aptamer was linked to the self-assembled β-CD and the sensor was immersed into the Pb²⁺ solution, the aptamers would combine with Pb²⁺ and then form G-quadruplex structures, which would cover the interspaces and then block the channels for probe entrance. The whole process reduced the oxidative current of probe, which provided a fundamental basis of Pb²⁺ determination in a quantitative way. Gate-controlled effect enhanced signal-to-noise ratio as well as sensitivity simultaneously. The differential pulse voltammetry response current showed a good linear relationship with the negative logarithm of Pb²⁺ concentration in the range from 1×10^-13 mol/L to 5×10^-11 mol/L, with a detection limit of 3.6×10^-14 mol/L(DL=3δ_b/K). The aptasensor was successfully applied to the detection of Pb²⁺ in real water samples with recoveries of 97.5%-103.3%.
- Electrochemical sensor,
- Gate-controlled effect,
- G-quadruplex,
- Lead ion(II)
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2. [2]
3. [3]
4. [4]
5. [5]
6. [6]
7. [7]
8. [8]
9. [9]
10. [10]
11. [11]
12. [12]
13. [13]
14. [14]
15. [15]
16. [16]
17. [17]
18. [18]
19. [19]
20. [20]
21. [21]
22. [22]
23. [23]
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