Citation: Lin LI, Jiaxue LI, Meixia YANG, Jiayu DING, Jiaqi JING, Ruiping ZHANG. Preparation of mitoxantrone self-assembled carrier-free nanodrugs regulated by sodium acetate for apoptosis induction of human breast carcinoma cells[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(12): 2536-2548. doi: 10.11862/CJIC.20250138 shu

Preparation of mitoxantrone self-assembled carrier-free nanodrugs regulated by sodium acetate for apoptosis induction of human breast carcinoma cells

Lin LI ^{1, 2,,} ,
Jiaxue LI ¹ ,
Meixia YANG ¹ ,
Jiayu DING ¹ ,
Jiaqi JING ¹ ,
Ruiping ZHANG ^2,,

1.
College of Chemistry and Materials, Taiyuan Normal University, Jinzhong, Shanxi 030619, China
2.
The Radiology Department of Shanxi Provincial People′s Hospital, Shanxi Medical University, Taiyuan 030012, China

Corresponding author: Lin LI, lilin@tynu.edu.cn Ruiping ZHANG, zrp_7142@sxmu.edu.cn
Received Date: 22 April 2025
Revised Date: 29 September 2025

Figures(11)

Based on the hydrogen bonding self-assembly strategy, a sodium acetate (NaAc) regulated high loading carrier-free nanodrug (MIT-DOX/NaAc, MIDA) was fabricated by employing a nano-coprecipitation method, which used mitoxantrone (MIT) and doxorubicin (DOX) as antitumor drug models. MIDA successfully formed with a maximum loading efficiency (95%), and the in vitro drug release showed that MIDA is pH-responsive. Excitingly, cytotoxicity and scratch migration assessments demonstrated that MIDA could target tumor cells through the enhanced permeability and retention effect (EPR), subsequently inhibiting the migration and proliferation of human breast carcinoma cells (MCF-7). The efficacy was higher than that of free DOX, MIT, and DOX/NaAc. Examination by fluorescence microscopy and flow cytometry showed that the uptake of MIDA by MCF-7 was time-dependent. In addition, MIDA could enter MCF-7 via both the clathrin and caveolae co-mediated endocytosis pathways, and then the dissociated MIT and DOX migrated into the nucleus to induce MCF-7 late apoptosis.
- mitoxantrone,
- carrier-free nanodrug,
- sodium acetate regulation,
- hydrogen bond self-assembly,
- human breast carcinoma cells
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