Citation: Yuting DU, Jing YUAN, Peiyao DENG. Synthesis and application of a fluorescent probe for the detection of reduced glutathione[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(7): 1331-1337. doi: 10.11862/CJIC.20240461 shu

Synthesis and application of a fluorescent probe for the detection of reduced glutathione

Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi 034000, China

Corresponding author: Yuting DU, yutingdu123@163.com
Received Date: 27 December 2024
Revised Date: 10 April 2025

Figures(7)

In this study, a fluorescent probe, CDAS, was synthesized to detect reduced glutathione (GSH). CDAS was designed by incorporating triphenylamine as an electron-donating group and a 2, 4-dinitrobenzenesulfonyl moiety as an electron-withdrawing group, with the response mechanism based on intramolecular charge transfer (ICT). These components were linked through Knoevenagel condensation. The probe exhibited no fluorescence; however, upon interaction with GSH, a nucleophilic substitution reaction occurred between GSH and probe CDAS, significantly enhancing fluorescent intensity at 561 nm. In a mixture of phosphate-buffered saline (PBS) and acetonitrile (1∶1, V/V, 10 mmol·L^-1, pH=7.4), CDAS demonstrated a low detection limit of 7.70 μmol·L^-1, high selectivity for GSH, and a large Stokes shift of 179 nm. Notably, CDAS can be effectively utilized to detect GSH in human cervical cancer cells (HeLa cells) via fluorescent cell imaging.
- triphenylamine,
- intramolecular charge transfer,
- fluorescent probe,
- reduced glutathione,
- cell imaging
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