Citation: Zeyu XU, Anlei DANG, Bihua DENG, Xiaoxin ZUO, Yu LU, Ping YANG, Wenzhu YIN. Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement[J]. Chinese Journal of Inorganic Chemistry, ;2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099 shu

Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement

Zeyu XU ^{1, 2, 3} ,
Anlei DANG ^{1, 2, 3} ,
Bihua DENG ^{1, 2} ,
Xiaoxin ZUO ^{1, 2} ,
Yu LU ^{1, 2, 3} ,
Ping YANG ^3,, ,
Wenzhu YIN ^{1, 2,,}

1.
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
2.
Guotai (Taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou, Jiangsu 225300, China
3.
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Corresponding author: Ping YANG, yangping@njau.edu.cn Wenzhu YIN, m18805157895@163.com
Received Date: 27 March 2024
Revised Date: 28 April 2024

Figures(8)

The improved Hummers method was used to obtain GOQDs from flake graphite, which contained rich hydroxyl and carboxyl groups. With the as-fabricated GOQDs in hand, we constructed a GOQDs/OVA nano-vaccine using chicken ovalbumin (OVA) as a model antigen to evaluate the immune efficacy and safety. Results showed that GOQDs/OVA nano-vaccine had high water dispersibility and stability with a diameter of around 5 nm for 30 d. The maximum loading capacity of GOQDs for OVA was about 500 mg·g^-1, and release rates of OVA were 74.65% and 56.93% in pH 5.5 and 7.4 after 24 h, respectively, displaying pH stimulus responsive release merits. With the concentration of GOQDs below 500 μg·mL^-1, the biosecurity of GOQDs indicated that they were not causing hemolysis, cell damage, and pathological changes in important tissues. After immunization, GOQDs/OVA nano-vaccines could excite the high levels of immunoglobulin G (IgG), immunoglobulin G1 (IgG1), and immunoglobulin G2a (IgG2a) antibodies and improve secretions of interleukin-1β (IL-1β), interleukin-4 (IL-2), interleukin-4 (IL-4), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interferon-γ (IFN-γ), compared with the group of OVA alone. Meanwhile, GOQDs promoted an increase in the percentage of CD4⁺ and CD8⁺ T lymphocytes in the spleen.
- graphene oxide,
- quantum dot,
- adjuvant,
- adsorption,
- antibody
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