Citation: Siyi ZHONG, Xiaowen LIN, Jiaxin LIU, Ruyi WANG, Tao LIANG, Zhengfeng DENG, Ao ZHONG, Cuiping HAN. Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots[J]. Chinese Journal of Inorganic Chemistry, ;2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093 shu

Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots

Siyi ZHONG ^{1, 2} ,
Xiaowen LIN ¹ ,
Jiaxin LIU ^{1, 2} ,
Ruyi WANG ^{1, 2} ,
Tao LIANG ^{1, 2} ,
Zhengfeng DENG ^{1, 2} ,
Ao ZHONG ^{1, 2} ,
Cuiping HAN ^{1, 3,,}

1.
School of Medical Imaging, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China
2.
National Demonstration Center for Experimental Teaching Basic Medicine Science Education, Xuzhou Medical University, Xuzhou, Jiangsu 221004, China
3.
Department of Radiology, the Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221000, China

Corresponding author: Cuiping HAN, hancp@xzhmu.edu.cn
Received Date: 25 March 2024
Revised Date: 24 June 2024

Figures(6)

Fluorescent magnetic gadolinium-doped carbon dots (Gd-CDs) were prepared using a high-temperature carbonization method. The prepared Gd-CDs emitted bright green fluorescence with a fluorescence quantum yield of 32.6% and a T1 relaxation efficiency of up to 9.02 L·mmol^-1·s^-1. By conjugating with the ovarian cancer-specific antibody Anti-HE4, the resulting Anti-HE4/Gd-CDs nanoprobes could perform specific fluorescent imaging and T1-weighted imaging of SKOV-3 ovarian cancer cells. The fluorescence intensity was proportional to the concentration of SKOV-3 cells, with a detection limit of 658 cell·mL^-1. This method was applied to the detection of SKOV-3 cells in blood samples.
- gadolinium-doped carbon dots,
- targeting probe,
- fluorescence imaging,
- magnetic resonance imaging,
- ovarian cancer cell
1. [1]
  Xia C F, Dong X S, Li H, Cao M M, Sun D Q, He S Y, Yang F, Yan X X, Zhang S L, Li N, Chen W Q. Cancer statistics in China and United States, 2022: Profiles, trends, and determinants[J]. Chin. Med. J., 2022,135(5):584-590. doi: 10.1097/CM9.0000000000002108
2. [2]
  TANG Z Y, DENG M G, YU C H, LIU Q. Current status and trend analysis of ovarian cancer disease burden in China[J]. Journal of International Obstetrics and Gynecology, 2022,49(2):222-227.
3. [3]
  Torre L A, Trabert B, DeSantis C E, Miller K D, Samimi G, Runowicz C D, Gaudet M M, Jemal A, Siegel R L. Ovarian cancer statistics, 2018[J]. CA-Cancer J. Clin., 2018,68(4):284-296. doi: 10.3322/caac.21456
4. [4]
  SHI S Y, LI Y A, WANG K Y, FANG Y H. Research progress in molecular imaging of ovarian cancer[J]. Journal of Practical Radiology, 2022,38(7):1193-1195.
5. [5]
  Weissleder R. Molecular imaging in cancer[J]. Science, 2006,312(5777):1168-1171. doi: 10.1126/science.1125949
6. [6]
  Zhuang D P, Zhang H F, Hu G W, Guo B. Recent development of contrast agents for magnetic resonance and multimodal imaging of glioblastoma[J]. J. Nanobiotechnol., 2022,20(1)284. doi: 10.1186/s12951-022-01479-6
7. [7]
  Wang C S, Wang Z H, Zhao T, Li Y, Huang G, Sumer B D, Gao J M. Optical molecular imaging for tumor detection and image-guided surgery[J]. Biomaterials, 2018,157:62-75. doi: 10.1016/j.biomaterials.2017.12.002
8. [8]
  Han C P, Zhang A W, Kong Y, Yu N N, Xie T, Dou B R, Li K, Wang Y, Li J J, Xu K. Multifunctional iron oxide-carbon hybrid nanoparticles for targeted fluorescent/MR dual-modal imaging and detection of breast cancer cells[J]. Anal. Chim. Acta, 2019,1067:115-128. doi: 10.1016/j.aca.2019.03.054
9. [9]
  LI S P, FENG X Y, LIANG C M, HE H J, MENG Q G, ZHAN C Y, LI J, GU B, SHE Z J, QIU L H, YANG B, CHEN L P. Synthesis, characterization and evaluation of BSA-(Gd-DTPA)_n as a MRI contrast agent precursor[J]. Chinese Computed Medical Imaging, 2010,16(1):46-51.
10. [10]
  Li H, Meade T J. Molecular magnetic resonance imaging with Gd(Ⅲ)-based contrast agents: Challenges and key advances[J]. J. Am. Chem. Soc., 2019,141(43):17025-17041. doi: 10.1021/jacs.9b09149
11. [11]
  Yano K, Matsumoto T, Okamoto Y, Kurokawa N, Hasebe T, Hotta A. Fabrication of Gd-DOTA-functionalized carboxylated nanodiamonds for selective MR imaging (MRI) of the lymphatic system[J]. Nanotechnology, 2021,32(23)235102. doi: 10.1088/1361-6528/abeb9c
12. [12]
  Chen X Y, Teng S Y, Li J M, Qiao X Z, Zhao W D, Xue Z J, Shi X D, Wang Y G, Yang W S, Wang T. Gadolinium(Ⅲ)-chelated deformable mesoporous organosilica nanoparticles as magnetic resonance imaging contrast agent[J]. Adv. Mater., 2023e2211578.
13. [13]
  Hu J Y, Sun Y Q, Aryee A A, Qu L B, Zhang K, Li Z H. Mechanisms for carbon dots-based chemosensing, biosensing, and bioimaging: A review[J]. Anal. Chim. Acta, 2022,1209338885. doi: 10.1016/j.aca.2021.338885
14. [14]
  Zheng S H, Yu N N, Han C P, Xie T, Dou B R, Kong Y, Zuo F M, Shi M L, Xu K. Preparation of gadolinium doped carbon dots for enhanced MR imaging and cell fluorescence labeling[J]. Biochem. Biophys. Res. Commun., 2019,511(2):207-213. doi: 10.1016/j.bbrc.2019.01.098
15. [15]
  Yu N N, Huang T H, Duan T F, Bao Y, Gao R C, Wang X Z, Xu K, Han C P. Accurate detection and delineation boundary of renal cell carcinoma based on dual-targeted magnetic-fluorescent carbon dots[J]. Chem. Eng. J., 2022,440135801. doi: 10.1016/j.cej.2022.135801
16. [16]
  Kwon W, Rhee S W. Facile synthesis of graphitic carbon quantum dots with size tunability and uniformity using reverse micelles[J]. Chem. Commun., 2012,48(43):5256-5258. doi: 10.1039/c2cc31687k
17. [17]
  Chen H M, Qiu Y W, Ding D D, Lin H R, Sun W J, Wang G D, Huang W C, Zhang W Z, Lee D, Liu G, Xie J, Chen X Y. Gadolinium-encapsulated graphene carbon nanotheranostics for imaging-guided photodynamic therapy[J]. Adv. Mater., 2018e1802748.
18. [18]
  Cui R L, Li J, Huang H, Zhang M Y, Guo X H, Chang Y, Li M, Dong J Q, Sun B Y, Xing G M. Novel carbon nanohybrids as highly efficient magnetic resonance imaging contrast agents[J]. Nano Res., 2015,8(4):1259-1268. doi: 10.1007/s12274-014-0613-x
19. [19]
  Zhang J, Fatouros P P, Shu C, Reid J, Owens L S, Cai T, Gibson H W, Long G L, Corwin F D, Chen Z J, Dorn H C. High relaxivity trimetallic nitride (Gd₃N) metallofullerene MRI contrast agents with optimized functionality[J]. Bioconjugate Chem., 2010,21(4):610-615. doi: 10.1021/bc900375n
20. [20]
  GAO Y L. Tumor marker for clinical application in ovarian cancer[J]. Journal of International Obstetrics and Gynecology, 2010,37(5):299-301.
21. [21]
  YANG J, DONG X C, ZHANG M J, WANG A H, HAI J. Value of human epididymal protein 4 to the diagnosis and prognosis assessment of ovarian cancer[J]. Journal of Chinese Practical Diagnosis and Therapy, 2021,35(8):819-821.
22. [22]
  Huang J B, Chen J Y, Huang Q Q. Diagnostic value of HE4 in ovarian cancer: A meta-analysis[J]. Eur. J. Obstet. Gynecol. Reprod. Biol., 2018,231:35-42.
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