Citation: Zheng-Dao HONG, Zhen-Hua LI, Ming XU, Wei FENG, Fu-You LI. Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(11): 2055-2064. doi: 10.11862/CJIC.2023.166 shu

Preparation of hollow polymer fluorescent microspheres and its application in lateral flow immunoassay

Zheng-Dao HONG ¹ ,
Zhen-Hua LI ¹ ,
Ming XU ² ,
Wei FENG ¹ ,
Fu-You LI ^2,,

1.
Department of Chemistry, Fudan University, Shanghai 200438, China
2.
Institute of Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: Fu-You LI, fyli@fudan.edu.cn
Received Date: 11 July 2023
Revised Date: 19 September 2023

Figures(9)

The functionalized polymer microspheres were prepared by soap-free emulsion polymerization using potassium persulfate (KPS) as the initiator, glycidyl methacrylate (GMA) and styrene (St) as monomers. It was found that microspheres prepared by adding a trace amount of NaCl could be swelled by tetrahydrofuran (THF) to obtain monodisperse hollow microspheres with complete surfaces. The Eu(Ⅲ) complex dye was then loaded into the microspheres shell by means of organic swelling, and immunochromatographic detection of α1 microglobulin (α1-MG) and β2 microglobulin (β2-MG) was performed.
- Eu(Ⅲ) complex,
- glycidyl methacrylate,
- styrene,
- hollow microspheres,
- lateral flow immunoassay
1. [1]
  Stuart M A C, Huck W T S, Genzer J, Muller M, Ober C, Stamm M, Sukhorukov G B, Szleifer I, Tsukruk V V, Urban M, Winnik F, Zauscher S, Luzinov I, Minko S. Emerging applications of stimuli-responsive polymer materials[J]. Nat. Mater., 2010,9(2):101-113. doi: 10.1038/nmat2614
2. [2]
  Nazli C, Demirer G S, Yar Y, Acar H Y, Kizilel S. Targeted delivery of doxorubicin into tumor cells via MMP-sensitive PEG hydrogel-coated magnetic iron oxide nanoparticles (MIONPs)[J]. Colloid Surf. B Biointerfaces, 2014,122:674-683. doi: 10.1016/j.colsurfb.2014.07.049
3. [3]
  Jiang Y Y, Cui D, Fang Y, Zhen X, Upputuri P K, Pramanik M, Ding D, Pu K Y. Amphiphilic semiconducting polymer as multifunctional nanocarrier for fluorescence/photoacoustic imaging guided chemophotothermal therapy[J]. Biomaterials, 2017,145:168-177. doi: 10.1016/j.biomaterials.2017.08.037
4. [4]
  Zhen X, Xie C, Pu K Y. Temperature-correlated afterglow of a semiconducting polymer nanococktail for imaging-guided photothermal therapy[J]. Angew. Chem Int. Ed., 2018,57(15):3938-3942. doi: 10.1002/anie.201712550
5. [5]
  Turkova J, Blaha K, Malanikova M, Vancurova D, Svec F, Kalal J. Methacrylate Gels with epoxide groups as supports for immobilization of enzymes in Ph range 3-12[J]. Biochim. Biophys. Acta, 1978,524(1):162-169. doi: 10.1016/0005-2744(78)90114-6
6. [6]
  Wang W C, Zhou W Q, Wei W, Li J, Hao D X, Su Z G, Ma G H. Towards a deeper understanding of the interfacial adsorption of enzyme molecules in gigaporous polymeric microspheres[J]. Polymers, 2016,8(4)116. doi: 10.3390/polym8040116
7. [7]
  Lou D D, Fan L, Cui Y, Zhu Y F, Gu N, Zhang Y. Fluorescent nanoprobes with oriented modified antibodies to improve lateral flow immunoassay of cardiac troponin Ⅰ[J]. Anal. Chem., 2018,90(11):6502-6508. doi: 10.1021/acs.analchem.7b05410
8. [8]
  Li Z H, Liu Q Y, Li Y F, Yuan W, Li F Y Y. One-step polymerized lanthanide-based polystyrene microsphere for sensitive lateral flow immunoassay[J]. J. Rare Earths, 2021,39(1):11-18. doi: 10.1016/j.jre.2020.06.020
9. [9]
  Khan A K, Ray B C, Maiti J, Dolui S K. Preparation of core-shell latex from co-polymer of styrene-butyl acrylate-methyl methacrylate and their paint properties[J]. Pigm. Resin Technol., 2009,38(3):159-164. doi: 10.1108/03699420910957015
10. [10]
  Nuasaen S, Tangboriboonrat P. Optical properties of hollow latex particles as white pigment in paint film[J]. Prog. Org. Coat., 2015,79:83-89. doi: 10.1016/j.porgcoat.2014.11.012
11. [11]
  Zhang Y Q, Li L L, Tang F Q, Ren J. Controlled drug delivery system based on magnetic hollow spheres/polyelectrolyte multilayer core-shell structure[J]. J. Nanosci. Nanotechnol., 2006,6(9/10):3210-3214.
12. [12]
  Chang F Q, Huang X B, Wei H, Chen K Y, Shan C C, Tang X Z. Intrinsically fluorescent hollow spheres based on organic-inorganic hybrid polyphosphazene material: Synthesis and application in drug release[J]. Mater. Lett., 2014,125:128-131. doi: 10.1016/j.matlet.2014.03.137
13. [13]
  Li G Y, Guo L, Wen Q W, Zhang T. Thermoand pH-sensitive ioniccrosslinked hollow spheres from chitosan-based graft copolymer for 5-fluorouracil release[J]. Int. J. Biol. Macromol., 2013,55:69-74. doi: 10.1016/j.ijbiomac.2012.12.048
14. [14]
  Fang K J, Zhang L, Cai Y Q, Hao L Y, Liu X M. Hollow disperse dyes/copolymer composite nanospheres[J]. Dyes Pigment., 2017,136:191-196. doi: 10.1016/j.dyepig.2016.08.037
15. [15]
  Parvate S, Dixit P, Chattopadhyay S. Hierarchical polymeric hollow microspheres with size tunable single holes and their application as catalytic microreactor[J]. Colloid. Polym. Sci., 2022,300(9):1101-1109. doi: 10.1007/s00396-022-05008-7
16. [16]
  Li Y B, Li Z H, Zheng F. Polyaniline hollow microspheres synthesized via self-assembly method in a polymer acid aqueous solution[J]. Mater. Lett., 2015,148:34-36. doi: 10.1016/j.matlet.2015.02.055
17. [17]
  Zhang Z P, Marson R L, Ge Z S, Glotzer S C, Ma P X. Simultaneous nano- and microscale control of nanofibrous microspheres self-assembled from star-shaped polymers[J]. Adv. Mater., 2015,27(26):3947-3952. doi: 10.1002/adma.201501329
18. [18]
  Sun Q H, Deng Y L. In situ synthesis of temperature-sensitive hol-low microspheres via interfacial polymerization[J]. J. Am. Chem. Soc., 2005,127(23):8274-8275. doi: 10.1021/ja051487k
19. [19]
  Suzuki T, Mizowaki T, Okubo M. Versatile synthesis of high performance, crosslinked polymer microcapsules with encapsulated n-hexadecane as heat storage materials by utilizing microsuspension controlled/living radical polymerization (ms CLRP) of ethylene glycol dimethacrylate with the SaPSeP method[J]. Polymer, 2016,106:182-188. doi: 10.1016/j.polymer.2016.08.109
20. [20]
  Lou X W, Archer L A, Yang Z C. Hollow micro-/nanostructures: Synthesis and applications[J]. Adv. Mater., 2008,20(21):3987-4019. doi: 10.1002/adma.200800854
21. [21]
  Wang R M, Lv S Y, Li T, He Y F, Song P F. Fabricating polymer microspheres through CaCO₃ templates[J]. Prog. Chem., 2016,28(1):75-82.
22. [22]
  Han J, Song G P, Guo R. Synthesis of polymer hollow spheres with holes in their surfaces[J]. Chem. Mat., 2007,19(5):973-975. doi: 10.1021/cm062686l
23. [23]
  Caruso F, Caruso R A, Mohwald H. Nanoengineering of inorganic and hybrid hollow spheres by colloidal templating[J]. Science, 1998,282(5391):1111-1114. doi: 10.1126/science.282.5391.1111
24. [24]
  Liu T W, Zhang W, Song T, Yang X L, Li C X. Hollow double-layered polymer microspheres with pH and thermo-responsive properties as nitric oxide-releasing reservoirs[J]. Polym. Chem., 2015,6(17):3305-3314. doi: 10.1039/C5PY00001G
25. [25]
  Shen J, Sun L D, Yan C H. Luminescent rare earth nanomaterials for bioprobe applications[J]. Dalton Trans., 2008(42):5687-5697. doi: 10.1039/b805306e
1. [1]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
2. [2]
  Zeyi Yan , Ruitao Liu , Xinyu Qi , Yuxiang Zhang , Lulu Sun , Xiangyuan Li , Anchao Feng . Exploration of Suspension Polymerization: Preparation and Fluorescence Stability of Perovskite Polystyrene Microbeads. University Chemistry, 2025, 40(4): 72-79. doi: 10.12461/PKU.DXHX202405110
3. [3]
  Chengqian Mao , Yanghan Chen , Haotong Bai , Junru Huang , Junpeng Zhuang . Photodimerization of Styrylpyridinium Salt and Its Application in Silk Screen Printing. University Chemistry, 2024, 39(5): 354-362. doi: 10.3866/PKU.DXHX202312014
4. [4]
  Haoqi Zhang , Borui Yao , Yun Zhang , Zhiguang Song , Bo Wang . 通过格氏试剂与环氧苯乙烯的反应认识其组成与性质的复杂性. University Chemistry, 2026, 41(5): 50-61. doi: 10.12461/PKU.DXHX202511129
5. [5]
  Ruoqian Zhang , Chaoqun Mu , Yali Hou , Mingming Zhang . 四苯乙烯基多组分金属有机笼的构筑及其固态发光性能研究. University Chemistry, 2025, 40(8): 277-283. doi: 10.12461/PKU.DXHX202410027
6. [6]
  Xiaopei HE , Jing HAN , Zhong YU , Na YE , Yi WAN . Preparation and antimicrobial properties of polyvinyl alcohol composite film based on Ag(Ⅰ) complex. Chinese Journal of Inorganic Chemistry, 2026, 42(3): 531-542. doi: 10.11862/CJIC.20250271
7. [7]
  Zhicheng JU , Wenxuan FU , Baoyan WANG , Ao LUO , Jiangmin JIANG , Yueli SHI , Yongli CUI . MOF-derived nickel-cobalt bimetallic sulfide microspheres coated by carbon: Preparation and long cycling performance for sodium storage. Chinese Journal of Inorganic Chemistry, 2025, 41(4): 661-674. doi: 10.11862/CJIC.20240363
8. [8]
  Ruiying WANG , Hui WANG , Fenglan CHAI , Zhinan ZUO , Benlai WU . Three-dimensional homochiral Eu(Ⅲ) coordination polymer and its amino acid configuration recognition. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 877-884. doi: 10.11862/CJIC.20250052
9. [9]
  Sihan Wang , Chenxi Yu , Shuzhang Ran , Jiawei Chen , Shoutong Rao , Xinyi Liang , Ruiqi Dong , Guixiang Zeng , Guoqiang Wang , Jing Ma . 铁配合物合成与性质探究的数智化改进——大学化学实验中“磺基水杨酸合铁配合物的组成与稳定常数的测定”的创新设计. University Chemistry, 2026, 41(5): 36-49. doi: 10.12461/PKU.DXHX202511137
10. [10]
  Xiaojun Liu , Lang Qin , Yanlei Yu . Dynamic Manipulation of Photonic Bandgaps in Cholesteric Liquid Crystal Microdroplets for Applications. Acta Physico-Chimica Sinica, 2024, 40(5): 2305018-0. doi: 10.3866/PKU.WHXB202305018
11. [11]
  Sumiya Akter Dristy , Md Ahasan Habib , Shusen Lin , Mehedi Hasan Joni , Rutuja Mandavkar , Young-Uk Chung , Md Najibullah , Jihoon Lee . Exploring Zn doped NiBP microspheres as efficient and stable electrocatalyst for industrial-scale water splitting. Acta Physico-Chimica Sinica, 2025, 41(7): 100079-0. doi: 10.1016/j.actphy.2025.100079
12. [12]
  Yu Peng , Jiawei Chen , Yue Yin , Yongjie Cao , Mochou Liao , Congxiao Wang , Xiaoli Dong , Yongyao Xia . Tailored cathode electrolyte interphase via ethylene carbonate-free electrolytes enabling stable and wide-temperature operation of high-voltage LiCoO₂. Acta Physico-Chimica Sinica, 2025, 41(8): 100087-0. doi: 10.1016/j.actphy.2025.100087
13. [13]
  Yue Dai , Yiwen Wang , Yu Wang , Mingxuan Liang , Yan Su . 基于微流控的镧系Eu-金属有机框架制备及荧光性能. University Chemistry, 2026, 41(5): 62-69. doi: 10.12461/PKU.DXHX202510029
14. [14]
  Zijian Zhao , Yanxin Shi , Shicheng Li , Wenhong Ruan , Fang Zhu , Jijun Jiang . A New Exploration of the Preparation of Polyacrylic Acid by Free Radical Polymerization Based on the Concept of Green Chemistry. University Chemistry, 2024, 39(5): 315-324. doi: 10.3866/PKU.DXHX202311094
15. [15]
  Xiutao Xu , Chunfeng Shao , Jinfeng Zhang , Zhongliao Wang , Kai Dai . Rational Design of S-Scheme CeO₂/Bi₂MoO₆ Microsphere Heterojunction for Efficient Photocatalytic CO₂ Reduction. Acta Physico-Chimica Sinica, 2024, 40(10): 2309031-0. doi: 10.3866/PKU.WHXB202309031
16. [16]
  Tong WANG , Qinyue ZHONG , Qiong HUANG , Weimin GUO , Xinmei LIU . Mn-doped carbon quantum dots/Fe-doped ZnO flower-like microspheres heterojunction: Construction and photocatalytic performance. Chinese Journal of Inorganic Chemistry, 2025, 41(8): 1589-1600. doi: 10.11862/CJIC.20250011
17. [17]
  Chengyan Ge , Jiawei Hu , Xingyu Liu , Yuxi Song , Chao Liu , Zhigang Zou . Self-integrated black NiO clusters with ZnIn₂S₄ microspheres for photothermal-assisted hydrogen evolution by S-scheme electron transfer mechanism. Acta Physico-Chimica Sinica, 2026, 42(1): 100154-0. doi: 10.1016/j.actphy.2025.100154
18. [18]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
19. [19]
  Zhao Feifan , Xu Feiyan , Yu Jiaguo . Interfacial stabilization of alkali metal oxides on carbon spheres for high-performance CO₂ chemisorption. Acta Physico-Chimica Sinica, 2026, 42(5): 100234-. doi: 10.1016/j.actphy.2025.100234
20. [20]
  Jinghua Wang , Yanxin Yu , Yanbiao Ren , Yesheng Wang . Integration of Science and Education: Investigation of Tributyl Citrate Synthesis under the Promotion of Hydrate Molten Salts for Research and Innovation Training. University Chemistry, 2024, 39(11): 232-240. doi: 10.3866/PKU.DXHX202402057

Get Citation

PDF

XML

Metrics

PDF Downloads(15)
Abstract views(3392)
HTML views(540)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142