Citation: Jing-Hong LIU, Qin GUI, Jian-Ming OUYANG. Effect of seaweed polysaccharides with different sulfate group contents on crystal growth of calcium oxalate and on the repair of damaged renal epithelial cells[J]. Chinese Journal of Inorganic Chemistry, ;2023, 39(3): 465-474. doi: 10.11862/CJIC.2023.015 shu

Effect of seaweed polysaccharides with different sulfate group contents on crystal growth of calcium oxalate and on the repair of damaged renal epithelial cells

College of Chemistry and Materials Science, Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: Jian-Ming OUYANG, toyjm@jnu.edu.cn
Received Date: 2 September 2022
Revised Date: 6 December 2022

Figures(9)

X-ray diffraction, Fourier transform infrared spectrometer, scanning electron microscope, ζ potential analyzer, and inductively coupled plasma emission spectrometer were used to compare the regulation of four degraded seaweed polysaccharides with a similar molecular weight of about 3700 Da and different contents of sulfate group (—OSO₃^-) on the inhibition of crystal growth of calcium oxalate (CaC₂O₄), and the repair ability of the polysaccharides on damaged renal epithelial cells was compared by cell experiments. These four seaweed polysaccharides were degraded Porphyra polysaccharide (PY-1), degraded Gracilaria lemaneiformis polysaccharide (GL-2), degraded Sargassum fusiforme polysaccharide (SF-3) and degraded Undaria pinnatifida polysaccharide (UP-4), and their —OSO₃^- contents were 17.9%, 13.3%, 8.2%, and 5.5%, respectively. The results showed that these four polysaccharides could inhibit the growth of calcium oxalate monohydrate (COM), induce the formation of calcium oxalate dihydrate (COD), increase the concentration of soluble Ca²⁺ ions in solution, and increase the absolute value of ζ potential on the crystal surface, thereby reducing the degree of crystal aggregation. In addition, these polysaccharides could repair HK-2 cells damaged by oxalic acid, increase cell viability and superoxide dismutase (SOD) activity, and reduce the release of malondialdehyde (MDA). The results show that the higher the content of —OSO₃^- in polysaccharides, the stronger the inhibitory effect of polysaccharides on CaC₂O₄ crystal growth and the repair effect on damaged HK-2 cells.
- seaweed polysaccharide,
- calcium oxalate,
- sulfate group content,
- crystallization inhibition
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