Advances in Cell Surface Shellization
- Corresponding author: WEI Yan, weiyan@tyut.edu.cn
Citation: SU Hui, XU Hao, ZHANG Li-Chuang, XU Shuang-Meng, WEI Yan, DU Jing-Jing, HUANG Di. Advances in Cell Surface Shellization[J]. Chinese Journal of Inorganic Chemistry, ;2019, 35(10): 1713-1727. doi: 10.11862/CJIC.2019.211
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E. coli are transformed with the silicatein gene (E. coli (pQE30-plus-silicatein)); After induction with IPTG E. coli express the silicatein protein; This protein is deposited on the cell surface of the bacteria where it mediates the formation of poly(silicate) after further incubation with monomeric silicic acid
After the silk nanoshell is formed, the yeast-enhanced green fluorescent protein (yEGFP) is expressed after incubation in an inducing molecule (galactose)-containing medium; the silk protein is gradually biodegraded and returned to the bare state without coating