Citation: RAO Chen-Ying, GUO Da, SUN Xin-Yuan, OUYANG Jian-Ming. Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells[J]. Chinese Journal of Inorganic Chemistry, ;2019, 35(3): 467-476. doi: 10.11862/CJIC.2019.039 shu

Toxicity Difference of Nano and Micron Calcium Oxalate Monohydrate on Renal Epithelial Cells

Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China

Corresponding author: OUYANG Jian-Ming, toyjm@jnu.edu.cn
Received Date: 12 October 2018
Revised Date: 28 November 2018

Figures(7)

Scanning electron microscope(SEM), laser confocal microscope(LSM), flow cytometry methods were used to comparatively study the toxicity difference of nano and micron calcium oxalate monohydrate(COM)on the African green monkey kidney epithelial(Vero)cells. The results revealed that both nano and micron COM could decrease cell viability, increase LDH release amount, up-regulate HA expression, decrease lysosomal integrity, decrease mitochondrial membrane potential, increased cell death rate and arrest cell cycle, which indicated that both nano and micron COM crystals could damage Vero cells and presented concentration dependent. But nano COM exhibited higher cytotoxicity and more adhesion amount than micron COM. This study may provide insights into the damage to renal epithelial cells induced by urinary crystals and the formation mechanism of kidney stones.
- Calcium oxalate,
- size effect,
- crystal adhesion,
- subcellular organelle injury
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