Citation: MAO Lan-Lan, ZHANG Li-Ming, DENG Yan, LÜ Zhuo-Xuan, HE Nong-Yue. Accurate Detection of DNA Based on the NaYF4: Yb, Er Upconversion Nanoparticles[J]. Chinese Journal of Inorganic Chemistry, ;2016, 32(12): 2095-2101. doi: 10.11862/CJIC.2016.271
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A new method for improving the accuracy of DNA detection has been developed, which was based on the base stacking principle and the fluorescence of NaYF4:Yb, Er UCNPs. Firstly, the NaYF4:Yb, Er UCNPs were synthesized by thermal decomposition method and functionalized with denatured bovine serum albumin. Then, the denatured bovine serum albumin-functionalized OA/NaYF4:Yb, Er UCNPs were conjugated with amino group-modified DNA probes to form upconversion fluorescence labeled probes and detect DNA. The results showed that the method of using the fluorescence of NaYF4:Yb, Er UCNPs as a reference standard to quantitatively detect target DNA concentration had higher accuracy than that of only using a single FAM fluorescence intensity, and the operation and equipment errors was effectively avoid during the experiment. Moreover, this method can reach the detection limit as low as 5 nmol·L-1 without amplification, displays a good linear relationship in wide concentration range and a high specificity, and also can effectively differenciate single-base mismatch sequences.
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