Citation: WEN Chang-Chun, LEI Wen-Qi, SHEN Xing-Can, JI Shi-Chen, JIANG Bang-Ping, LIANG Hong. Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(9): 1903-1912. doi: 10.11862/CJIC.2015.253 shu

Comparative Interaction Mechanisms Between Cells and Gold Nanoparticles Modified with Different Chemical Functional Groups

1.
Key Laboratory of Medicinal Chemical Resources and Molecular Engineering, Ministry of Education, Guangxi Normal University, Guilin 541004, China

Corresponding author: SHEN Xing-Can, LIANG Hong,
Received Date: 18 June 2015
Available Online: 4 August 2015
Fund Project: 国家自然科学基金(No.21161003,21364002) (No.21161003,21364002)广西自然科学基金杰青(2013GXNSFGA019001) (2013GXNSFGA019001)教育部新世纪优秀人才支持计划(NCET-13-0743) (NCET-13-0743)药用资源化学与药物分子工程教育部重点实验室主任基金(2015-A)资助项目。 (2015-A)

Chemical functional groups of -CH₃, -COOH and -OH have been introduced to the surface of AuNPs, separately. The AuNPs, Au-OH NPs, Au-COOH NPs and Au-CH₃ NPs are spherical with dimension of (15.6±3.2) nm, displaying negative ζ potentials. The cytotoxicity of these AuNPs has been evaluated by methylthiazoletetrazolium (MTT) assay against Hela cells and MCG-803 cells in vitro, separately. MTT data reveal that the surface unmodified AuNPs exhibit low cytotoxicity at the highest concentration of 197 ng·mL^-1 for both HeLa and MCG-803 cells in vitro. The surface modified AuNPs can further decrease the inherently cytotoxicity that follows the order AuNPs > Au-CH₃ NPs > Au-COOH NPs≈Au-OH NPs. Cell cycle analysis indicates that AuNPs cause cell cycle slightly arrest at the G₂/M phase. Micro-Raman spectra of individual living HeLa cells demonstrate that the backbone and nucleic bases of DNA as well as the polar headgroup of phospholipid in cells are the probable target binding sites of AuNPs and Au-CH₃ NPs. Whereas, the interfacial interactions are significantly reduced when cells are treated with Au-COOH NPs and Au-OH NPs. Our results on the interaction mechanisms between AuNPs and cells demonstrate that AuNPs modified with surface functional groups of -COOH or -OH can improve their cytocompatibility.
- gold nanoparticles (AuNPs);surface functional groups;cycotoxicity;molecular mechanisms
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沈阳化工大学材料科学与工程学院沈阳 110142