Citation: TANG Qian, SHI Shan-Shan, CAO Hong-Yu, GUO Xiang-Jin, ZHANG Tao, ZHENG Xue-Fang. Reaction Mechanism of Cytochrome C with NO[J]. Chinese Journal of Inorganic Chemistry, ;2015, 31(8): 1511-1519. doi: 10.11862/CJIC.2015.216 shu

Reaction Mechanism of Cytochrome C with NO

TANG Qian ^1,2 ,
SHI Shan-Shan ^1,2 ,
CAO Hong-Yu ^1,2 ,
GUO Xiang-Jin ² ,
ZHANG Tao ¹ ,
ZHENG Xue-Fang ^1,2,,

1.
1. College of Life Science and Biotechnology, Dalian University, Dalian, Liaoning 116622, China;
2.
2. Liaoning Key Laboratory of Bio-organic Chemistry, Dalian University, Dalian, Liaoning 116622, China

Corresponding author: ZHENG Xue-Fang,
Received Date: 5 September 2014
Available Online: 7 May 2015
Fund Project: 国家自然科学基金 (No.21271036, 20871024) (No.21271036, 20871024)辽宁省教育厅科学技术研究 (No.L2013470, L2013471)资助项目。 (No.L2013470, L2013471)

The research of cytochrome C (Cyt C) reacting with NO donor drugs(proliNONOate) has been focused on electrochemistry and medical treatment, while the study about reacting with NO gas is ignored. The previous study usually concentrated on Q band changes, but the Soret band was hardly mentioned. In this paper, we studied the reaction of two states of Cyt C with NO gas and the dissociation process by Soret band and Q band spectra, using ultraviolet-visible absorption spectra, electron paramagnetic resonance (EPR) spectra, ultraviolet-visible time course absorption spectra and synchronous fluorescence. The spectroscopic data showed that, ferric cytochrome C(Fe(Ⅲ)-Cyt C) and ferrous cytochrome C(Fe(Ⅱ)-Cyt C) could react with NO to convert to cytochrome C coordination compound(Cyt C-NO) with different mechanisms. Fe(Ⅱ)-Cyt C reacting with NO generated Fe(Ⅲ)-Cyt C firstly, then it combined with NO, while Fe(Ⅲ)-Cyt C can combine with NO directly. However, Cyt C-NO is not a stable coordinated macromolecule. With a small amount of NO bubbled into the sample, Cyt C-NO dissociated rapidly with rate constant value of (0.005 07±0.001) s^-1, which is one-tenth of Cyt C-NO with NO donor drugs; while under excessive NO gas condition, the dissociation process was prevented. According to the experimental data, the coordination mechanism of the reaction between Cyt C and NO is that gas molecule gets access into the cavity of Heme, making the separation of Fe-S and the formation of Fe-N. Cyt C can react with NO gas directly, the Cyt C-NO is more stable than that reacted with NO donor drugs, and the Soret band have an obvious change. These results will be significant for us to remit the oxidative stress in cells and detect the respiratory enzymes change which could monitor the cell apoptosis and some diseases by NO.
- cytochrome c;nitric oxide;spectrometry;coordination reaction;dissociation
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