Citation: JIAO Yuan-Hong, ZHANG Qian, JIANG Yu-Feng, ZHANG Wan-Ju. A Benzothiazole-Derived Fluorescent Probe for Detecting Hg(Ⅱ) in Live Cells[J]. Chinese Journal of Inorganic Chemistry, ;2015, (2): 361-368. doi: 10.11862/CJIC.2015.019 shu

A Benzothiazole-Derived Fluorescent Probe for Detecting Hg(Ⅱ) in Live Cells

1.
1. College of Chemistry and Chemical Engineering, Hubei Polytechnic University, Huangshi, Hubei 435003, China;
2.
2. Hubei Key Laboratory for Processing and Application of Catalytic Materials, Huanggang Normal University, Huanggang, Hubei 438000, China;
3.
3. Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Hubei Polytechnic University, Huangshi, Hubei 435003, China

Corresponding author: ZHANG Wan-Ju,
Received Date: 29 August 2014
Available Online: 25 October 2014
Fund Project: 湖北省教育厅B类项目(No.B20114404) (No.B20114404)矿区环境污染控制与修复湖北省重点实验室开放基金项目(No.2012103) (No.2012103)湖北省教育厅青年人才资助项目(No.Q20132901) (No.Q20132901)

Abenzothiazole-derived fluorescent probe (YH1) has been synthesized and structurally characterized, and its response to different metal ions has been investigated by spectrometry. The results showed that YH1 exhibited good sensitivity and selectivity to Hg²⁺. Its interaction with Hg²⁺ caused the color of its solution changed from blue to colorless under UVirradiation. There was a good linear relationship between the fluorescence intensity of YH1 and the concentration of Hg²⁺ in the range of 1.4 μmol·L^-1 to 8.8 μmol·L^-1, and its detection limit for Hg²⁺ was 0.56 μmol·L^-1. Moreover, the cell membrane penetration of YH1 had low cytotoxicity, and it can be applied to image intracellular Hg²⁺ in living HeLa cells. CCDC: 978108.
- crystal structure;Hg²⁺;fluorescent probe;benzothiazole;fluorescent imaging
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