Citation: Ting Huang, Yan Chen, Peng-fei Sun, Qu-li Fan, Wei Huang. Conjugated-polymer Nanoparticle for NIR-II Fluorescence Imaging Guiding NIR-II Photothermal Therapy[J]. Acta Polymerica Sinica, ;2020, 51(4): 346-354. doi: 10.11777/j.issn1000-3304.2019.19192 shu

Conjugated-polymer Nanoparticle for NIR-II Fluorescence Imaging Guiding NIR-II Photothermal Therapy

Key Laboratory for Organic Electronics & Information Displays, Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023

Corresponding author: Peng-fei Sun, iampfsun@njupt.edu.cn Qu-li Fan, iamqlfan@njupt.edu.cn
Received Date: 14 November 2019
Revised Date: 25 December 2019
Available Online: 22 January 2020

Figures(7)

To improve the quality of fluorescence imaging and effectiveness of photothermal therapy, We designed a novel conjugated-polymer (BDT-TTQ) with a narrow band gap for NIR-II fluorescence and NIR-II photothermal effect. To realize solubility of BDT-TTQ in water, we enveloped the hydrophobic polymer BDT-TTQ into amphiphilic copolymer (PEG-b-PPG-b-PEG, F-127) shells for NIR-II water-soluble nanoparticles (BDT-TTQ NPs) through the nanoprecipitation method. With strong absorption in the NIR-II region of 1000 − 1200 nm, the BDT-TTQ NPs can realize fluorescence image during 1200 − 1400 nm excited by 1064 nm laser. The prepared BDT-TTQ NPs were characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The average hydrodynamic radius of BDT-TTQ NPs was around 62 nm and spherical morphology was observed from TEM. Besides, the BDT-TTQ NPs showed the similar hydrodynamic radius in physiological environment such as phosphate buffer saline (PBS), Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) and exhibited the excellent biological stability, indicating the potential for further in vivo application. To study the NIR-II fluorescence characters, we firstly detected the maximum imaging depth of BDT-TTQ NPs in vitro and the penetration depth can achieve 6 mm at 1064 nm laser. High-resolution NIR-II fluorescence imaging of living blood vessels in mice was also achieved by BDT-TTO NPs under 1064 nm laser irradiation. In addition, the real-time NIR-II images of brain and abdomen were obtained with an ultrahigh signal-to-background ratio. Photothermal experiments suggested the BDT-TTQ NPs exhibited excellent photothermal conversion and outstanding photothermla stability under 1064 nm excitation, which revealed the potential of BDT-TTQ NPs for photothermal therapy in vivo. MTT assay and confocal laser scanning microscopy (CLSM) were used to analyse photothermal treatment toward human cervical carcinoma (HeLa) cells in vitro and the results indicated the nanoparticles performed an effective photothermal inhibition at the cellular level under laser irradiation. Morever, BDT-TTQ NPs developed in this study can realize NIR-II fluorescence imaging-guided photothermal therapy in vivo at 1064 nm laser.
- Conjugated-polymer,
- 1064 nm Excitation,
- NIR-II fluorescence imaging,
- NIR-II photothermal
1. [1]
  Zhang Jianxu(张剑旭), Sun Tingting(孙婷婷), Xie Zhigang(谢志刚). Acta Polymerica Sinica(高分子学报), 2019, 50(6): 633 − 641
2. [2]
  Yan Gang(严刚), Nie Guangting(聂光庭), Kong Xiangquan(孔祥权), Kuang Miao(邝淼), Rong Jianghua(容建华). Acta Polymerica Sinica(高分子学报), 2016, (2): 256 − 263
3. [3]
  Ge X G, Fu Q R, Bai L, Chen B, Wang R J, Gao S, Song J B. New J Chem, 2019, 43: 8835 − 8851 doi: 10.1039/C9NJ01402K
4. [4]
  Zhou J J, Jiang Y Y, Hou S A, Upputuri P K, Wu D, Li J C, Wang P. ACS Nano, 2018, 12: 2643 − 2651 doi: 10.1021/acsnano.7b08725
5. [5]
  Deng X R, Liang S, Cai X C, Huang S S, Cheng Z Y, Shi Y S, Pang M L, Ma P A, Lin J. Nano Lett, 2019, 19: 6772 − 6780 doi: 10.1021/acs.nanolett.9b01716
6. [6]
  Lin H, Gao S S, Dai C, Chen Y, Shi J L. J Am Chem Soc, 2017, 139: 16235 − 16247 doi: 10.1021/jacs.7b07818
7. [7]
  Yu X J, Yang K, Chen X Y, Li W W. Biomaterials, 2017, 143: 120 − 129 doi: 10.1016/j.biomaterials.2017.07.037
8. [8]
  Zhou L B, Jing Y, Liu Y B, Liu Z H, Gao D Y, Chen H B, Song W Y, Wang T, Fang X F, Qin W P, Yuan Z, Dai S, Qiao Z A, Wu C F. Theranostics, 2018, 8: 663 − 675 doi: 10.7150/thno.21927
9. [9]
  Chen Q S, Chen J Q, He M, Bai Y Y, Yan H X, Zeng N, Liu F Y, Wen S, Song L, Sheng Z H, Liu C B, Fang C H. Biomater Sci, 2019, 7: 3165 − 3177 doi: 10.1039/C9BM00528E
10. [10]
  Sun T T, Han J F, Liu S, Wang X, Wang Z Y, Xie Z G. ACS Nano, 2019, 13: 7345 − 7354 doi: 10.1021/acsnano.9b03910
11. [11]
  Zhang W S, Huang T, Li J W, Sun P F, Wang Y F, Shi W, Han W, Wang W J, Fan Q L, Huang W. ACS Appl Mater Interfaces, 2019, 11: 16311 − 16319 doi: 10.1021/acsami.9b02597
12. [12]
  Lu X M, Yuan P C, Zhang W S, Wu Q, Wang X X, Zhao M, Sun P F, Huang W, Fan Q L. Polym Chem, 2018, 9: 3118 − 3126 doi: 10.1039/C8PY00215K
13. [13]
  Li Xiaozhen(李晓珍), Tian Sichao(田思超), Sun Pengfei(孙鹏飞), Fan Quli(范曲立), Huang Wei(黄维). Acta Polymerica Sinica(高分子学报), 2017, (6): 952 − 958 doi: 10.11777/j.issn1000-3304.2017.16311
14. [14]
  Sun Pengfei(孙鹏飞), Deng Weixing(邓卫星), Fan Quli(范曲立), Huang Wei(黄维). Acta Polymerica Sinica(高分子学报), 2016, (3): 375 − 381 doi: 10.11777/j.issn1000-3304.2016.15255
15. [15]
  Li C Y, Zhang Y J, Chen G C, Hu F, Zhao K, Wang Q B. Adv Mater, 2017, 29: 1605754 doi: 10.1002/adma.201605754
16. [16]
  Lu Xiaomei(卢晓梅), Zhao Meng(赵萌), Huang Wei(黄维). Acta Polymerica Sinica(高分子学报), 2018, (11): 1451 − 1458 doi: 10.11777/j.issn1000-3304.2018.18068
17. [17]
  Yang Q L, Ma Z R, Wang H S, Zhou B, Zhu S J, Zhong Y T, Wang J Y, Wan H, Antaris A, Ma R, Zhang X, Yang J Y, Zhang X D, Sun H T, Liu W Q, Liang Y Y, Dai H J. Adv Mater, 2017, 29: 1605497 doi: 10.1002/adma.201605497
18. [18]
  Zhang X D, Wang H S, Antaris A L, Li L L, Diao S, Ma R, Nguyen A, Hong G S, Ma Z R, Wang J, Zhu S J, Castellano J M, Wyss-Coray T, Liang Y Y, Luo J, Dai H J. Adv Mater, 2016, 28: 6872 − 6879 doi: 10.1002/adma.201600706
19. [19]
  Li C Y, Zhang Y J, Wang M, Zhang Y, Chen G C, Li L, Wu D M, Wang Q B. Biomaterials, 2014, 35: 393 − 400 doi: 10.1016/j.biomaterials.2013.10.010
20. [20]
  Hao X X, Li C Y, Zhang Y J, Wang H Z, Chen G C, Wang M, Wang Q B. Adv Mater, 2018, 30: 1804437 doi: 10.1002/adma.201804437
21. [21]
  Dong B H, Li C Y, Chen G C, Zhang Y J, Zhang Y, Deng M J, Wang Q B. Chem Mater, 2013, 25: 2503 − 2509 doi: 10.1021/cm400812v
22. [22]
  Guo B, Sheng Z H, Hu D H, Liu C B, Zheng H R, Liu B. Adv Mater, 2018, 30: 1802591 doi: 10.1002/adma.201802591
23. [23]
  Sun T T, Dou J H, Liu S, Wang X, Zheng X H, Wang Y P, Jian Pei, Xie Z G. ACS Appl Mater Interfaces, 2018, 10: 7919 − 7926 doi: 10.1021/acsami.8b01458
24. [24]
  Zhang W S, Sun X L, Huang T, Pan X X, Sun P F, Li J W, Zha H, Lu X M, Fan Q L, Huang W. Chem Commun, 2019, 55: 9487 doi: 10.1039/C9CC04196F
25. [25]
  Qi J, Sun C W, Li D Y, Zhang H Q, Yu W B, Zebibula A, Lam J W Y, Xi W, Zhu L, Cai F H, Wei P F, Zhu C L, Kwok R T K, Streich L L, Prevedel R, Qian J, Tang B Z. ACS Nano, 2018, 12: 7936 − 7945 doi: 10.1021/acsnano.8b02452
26. [26]
  Li B H, Lu L F, Zhao M Y, Lei Z H, Zhang F. Angew Chem Int Ed, 2018, 57: 7483 − 7487 doi: 10.1002/anie.201801226
1. [1]
  Qi Wang , Yicong Gao , Feng Lu , Quli Fan . Preparation and Performance Characterization of the Second Near-Infrared Phototheranostic Probe: A New Design and Teaching Practice of Polymer Chemistry Comprehensive Experiment. University Chemistry, 2024, 39(11): 342-349. doi: 10.12461/PKU.DXHX202404141
2. [2]
  Mian Wei , Chang Cheng , Bowen He , Bei Cheng , Kezhen Qi , Chuanbiao Bie . Inorganic-organic CdS/YBTPy S-scheme photocatalyst for efficient hydrogen production and its mechanism. Acta Physico-Chimica Sinica, 2025, 41(12): 100158-0. doi: 10.1016/j.actphy.2025.100158
3. [3]
  Ke Qiu , Fengmei Wang , Mochou Liao , Kerun Zhu , Jiawei Chen , Wei Zhang , Yongyao Xia , Xiaoli Dong , Fei Wang . A Fumed SiO₂-based Composite Hydrogel Polymer Electrolyte for Near-Neutral Zinc-Air Batteries. Acta Physico-Chimica Sinica, 2024, 40(3): 2304036-0. doi: 10.3866/PKU.WHXB202304036
4. [4]
  Xue Wu , Yupeng Liu , Bingzhe Wang , Lingyun Li , Zhenjian Li , Qingcheng Wang , Quansheng Cheng , Guichuan Xing , Songnan Qu . Rationally assembling different surface functionalized carbon dots for enhanced near-infrared tumor photothermal therapy. Acta Physico-Chimica Sinica, 2025, 41(9): 100109-0. doi: 10.1016/j.actphy.2025.100109
5. [5]
  Jiahui CHEN , Tingting ZHENG , Xiuyun ZHANG , Wei LÜ . Research progress of near-infrared absorption inorganic nanomaterials in photothermal and photodynamic therapy of tumors. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2396-2414. doi: 10.11862/CJIC.20240106
6. [6]
  Ruonan Li , Shijie Liang , Yunhua Xu , Cuifen Zhang , Zheng Tang , Baiqiao Liu , Weiwei Li . Chlorine-Substituted Double-Cable Conjugated Polymers with Near-Infrared Absorption for Low Energy Loss Single-Component Organic Solar Cells. Acta Physico-Chimica Sinica, 2024, 40(8): 2307037-0. doi: 10.3866/PKU.WHXB202307037
7. [7]
  Han ZHANG , Jianfeng SUN , Jinsheng LIANG . Hydrothermal synthesis and luminescent properties of broadband near-infrared Na₃CrF₆ phosphor. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 349-356. doi: 10.11862/CJIC.20240098
8. [8]
  Zhongxin YU , Wei SONG , Yang LIU , Yuxue DING , Fanhao MENG , Shuju WANG , Lixin YOU . Fluorescence sensing on chlortetracycline of a Zn-coordination polymer based on mixed ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2415-2421. doi: 10.11862/CJIC.20240304
9. [9]
  .
  南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积
  . CCS Chemistry, 2025, 7(0): -.
10. [10]
  Xiao SANG , Qi LIU , Jianping LANG . Synthesis, structure, and fluorescence properties of Zn(Ⅱ) coordination polymers containing tetra-alkenylpyridine ligands. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2124-2132. doi: 10.11862/CJIC.20240158
11. [11]
  Minghui Wu , Markus Mühlinghaus , Xuechao Li , Chaojie Xu , Qiang Chen , Haiming Zhang , Klaus Müllen , Lifeng Chi . On-Surface Synthesis of Chevron-Shaped Conjugated Ladder Polymers Consisting of Benzo[a]azulene Units. Acta Physico-Chimica Sinica, 2024, 40(8): 2307024-0. doi: 10.3866/PKU.WHXB202307024
12. [12]
  Fanpeng Meng , Fei Zhao , Jingkai Lin , Jinsheng Zhao , Huayang Zhang , Shaobin Wang . Optimizing interfacial electric fields in carbon nitride nanosheet/spherical conjugated polymer S-scheme heterojunction for hydrogen evolution. Acta Physico-Chimica Sinica, 2025, 41(8): 100095-0. doi: 10.1016/j.actphy.2025.100095
13. [13]
  You Wu , Chang Cheng , Kezhen Qi , Bei Cheng , Jianjun Zhang , Jiaguo Yu , Liuyang Zhang . Efficient Photocatalytic Production of H₂O₂ over ZnO/D-A Conjugated Polymer S-scheme Heterojunction and Charge Transfer Dynamics Investigation. Acta Physico-Chimica Sinica, 2024, 40(11): 2406027-0. doi: 10.3866/PKU.WHXB202406027
14. [14]
  Yuxia Luo , Xiaoyu Xie , Fangfang Chen . 药物递送魔法师——分子印迹聚合物. University Chemistry, 2025, 40(8): 202-210. doi: 10.12461/PKU.DXHX202409129
15. [15]
  Bao Jia , Yunzhe Ke , Shiyue Sun , Dongxue Yu , Ying Liu , Shuaishuai Ding . Innovative Experimental Teaching for the Preparation and Modification of Conductive Organic Polymer Thin Films in Undergraduate Courses. University Chemistry, 2024, 39(10): 271-282. doi: 10.12461/PKU.DXHX202404121
16. [16]
  Xuefei Leng , Yanshai Wang , Hai Wang , Shengyang Tao . The In-Depth integration of “Industry-University-Research” in the Exploration and Practice of “Comprehensive Training in Polymer Engineering”. University Chemistry, 2025, 40(4): 66-71. doi: 10.12461/PKU.DXHX202405105
17. [17]
  Ruiying WANG , Hui WANG , Fenglan CHAI , Zhinan ZUO , Benlai WU . Three-dimensional homochiral Eu(Ⅲ) coordination polymer and its amino acid configuration recognition. Chinese Journal of Inorganic Chemistry, 2025, 41(5): 877-884. doi: 10.11862/CJIC.20250052
18. [18]
  Chunyuan Kang , Xiaoyu Li , Fan Yang , Bai Yang . Ionic-bond crosslinked carbonized polymer dots for tunable and enhanced room temperature phosphorescence. Acta Physico-Chimica Sinica, 2026, 42(1): 100156-0. doi: 10.1016/j.actphy.2025.100156
19. [19]
  Ran HUO , Zhaohui ZHANG , Xi SU , Long CHEN . Research progress on multivariate two dimensional conjugated metal organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2063-2074. doi: 10.11862/CJIC.20240195
20. [20]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084

Get Citation

PDF

XML

Metrics

PDF Downloads(144)
Abstract views(7563)
HTML views(1340)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Conjugated-polymer Nanoparticle for NIR-II Fluorescence Imaging Guiding NIR-II Photothermal Therapy

南开大学师唯/华北电力大学（保定）刘景维：二维配位聚合物中有序的亲锂冠醚位点用于无枝晶锂沉积

Metrics

通讯作者: 陈斌, bchen63@163.com