Citation: Weiwei Liu, Yu Liu, Zhaoyan Tian, Zhaohan Wang, Hui Liu, Songqin Liu, Yafeng Wu. Online detecting living cells released TNF-α and studying intercellular communication using SuperDNA self-assembled conical nanochannel[J]. Chinese Chemical Letters, ;2025, 36(5): 110561. doi: 10.1016/j.cclet.2024.110561 shu

Online detecting living cells released TNF-α and studying intercellular communication using SuperDNA self-assembled conical nanochannel

Weiwei Liu ^a ,
Yu Liu ^a ,
Zhaoyan Tian ^b ,
Zhaohan Wang ^a ,
Hui Liu ^a ,
Songqin Liu ^a ,
Yafeng Wu ^{a, *,}

a.
Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
b.
State Key Laboratory for Macromolecule Drugs and Large-scale Manufacturing, School of Pharmaceutical Sciences, Liaocheng University, Liaocheng 252059, China

wuyafeng@seu.edu.cn

Received Date: 23 June 2024
Revised Date: 23 September 2024
Accepted Date: 17 October 2024
Available Online: 18 October 2024

Figures(6)

Nanochannel technology based on ionic current rectification has emerged as a powerful tool for the detection of biomolecules owing to unique advantages. Nevertheless, existing nanochannel sensors mainly focus on the detection of targets in solution or inside the cells, moreover, they only have a single function, greatly limiting their application. Herein, we fabricated SuperDNA self-assembled conical nanochannel, which was clamped in the middle of self-made device for two functions: Online detecting living cells released TNF-α and studying intercellular communication. Polyethylene terephthalate (PET) membrane incubated tumor associated macrophages and tumor cells was rolled up and inserted into the left and right chamber of the device, respectively. Through monitoring the ion current change in the nanochannel, tumor associated macrophages released TNF-α could be in situ and noninvasive detected with a detection limit of 0.23 pg/mL. Furthermore, the secreted TNF-α induced epithelial-mesenchymal transformation of tumor cells in the right chamber was also studied. The presented strategy displayed outstanding performance and multi-function, providing a promising platform for in situ non-destructive detection of cell secretions and related intercellular communication analysis.
- Nanochannel,
- Ion transport,
- In situ detection,
- Cell secretions,
- Intercellular communication
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