Citation: Xianzhu Luo, Feifei Yu, Rui Wang, Tian Su, Pan Luo, Pengfei Wen, Fabiao Yu. A near-infrared two-photon fluorescent probe for the detection of HClO in inflammatory and tumor-bearing mice[J]. Chinese Chemical Letters, ;2025, 36(7): 110531. doi: 10.1016/j.cclet.2024.110531 shu

A near-infrared two-photon fluorescent probe for the detection of HClO in inflammatory and tumor-bearing mice

Xianzhu Luo ^{a, d, 1} ,
Feifei Yu ^{a, d, 1} ,
Rui Wang ^{a, d} ,
Tian Su ^{a, d} ,
Pan Luo ^c ,
Pengfei Wen ^{b, *,} ,
Fabiao Yu ^{a, d, *,}

a.
Key Laboratory of Emergency and Trauma, Ministry of Education, Key Laboratory of Haikou Trauma, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou 571199, China
b.
Department of Joint Surgery, HongHui Hospital, Xi’an Jiaotong University, Xi’an 710054, China
c.
Department of Comprehensive Plastic Surgery, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100144, China
d.
Engineering Research Center for Hainan Bio-Smart Materials and Bio-Medical Devices, Key Laboratory of Hainan Functional Materials and Molecular Imaging, College of Emergency and Trauma, College of Pharmacy, Hainan Medical University, Haikou 571199, China

wenpengfei@pku.edu.cn

yufabiao@muhn.edu.cn

Received Date: 26 August 2024
Revised Date: 30 September 2024
Accepted Date: 8 October 2024
Available Online: 10 October 2024

Figures(5)

Hypochlorous acid (HClO) is a critical biomolecule in living organisms, playing an essential role in numerous physiological or pathological processes. Abnormal levels of HClO in the body may lead to a series of diseases, for instance, inflammation and cancer. Thus, accurate measurement of HClO levels should be more beneficial for understanding its role in diseases and gaining a deeper insight into the pathogenesis of diseases. In this work, we designed a near-infrared two-photon fluorescent probe (HDM-Cl-HClO) for detecting fluctuations in HClO levels in inflammatory and tumor-bearing mice. Notably, the probe can respond to HClO within 5 s and trigger a brilliant red fluorescence at 660 nm. It exhibits high specificity and sensitivity for HClO. The superior spectral capability of the probe has enabled the detection of HClO levels in cells and zebrafish, as well as achieved the detection of HClO in inflammatory and tumor mice. This work not only provides a novel strategy and tool for HClO imaging in living systems, but also holds great potential for the diagnosis of inflammation and cancer.
- Hypochlorous acid,
- Near-infrared,
- Two-photon,
- Fluorescent probe,
- Inflammation,
- Tumor
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