Citation: Jianju Li, Xinwei Chen, Yang Yu, Hao Ma, Xinhui Xia, Zixuan Zhao, Junqiu Jiang, Qingliang Zhao, Yingzi Lin, Liangliang Wei. Insights into bioavailable heavy metal impact driven by sludge application on soil nitrification: Toxicity thresholds and influential factors[J]. Chinese Chemical Letters, ;2025, 36(7): 110410. doi: 10.1016/j.cclet.2024.110410 shu

Insights into bioavailable heavy metal impact driven by sludge application on soil nitrification: Toxicity thresholds and influential factors

Jianju Li ^a ,
Xinwei Chen ^a ,
Yang Yu ^{a, c} ,
Hao Ma ^a ,
Xinhui Xia ^a ,
Zixuan Zhao ^a ,
Junqiu Jiang ^a ,
Qingliang Zhao ^a ,
Yingzi Lin ^b ,
Liangliang Wei ^{a, b,,}

a.
State Key Laboratory of Urban Water Resources and Environment (SKLUWRE), School of Environment, Harbin Institute of Technology, Harbin 150090, China
b.
Key Laboratory of Songliao Aquatic Environment, Ministry of Education, School of Municipal and Environmental Engineering, Jilin Jianzhu University, Changchun 130118, China
c.
China Construction Eighth Engineering Division Co., Ltd., Shanghai 200112, China

Corresponding author: Liangliang Wei, weill333@163.com
Received Date: 23 April 2024
Revised Date: 6 August 2024
Accepted Date: 4 September 2024
Available Online: 4 September 2024

Figures(6)

Strict regulations on heavy metal (HM) limits impede the sludge land utilization for carbon emission reduction. This study aimed to evaluate the impact of bioavailable HMs (Cd, Cu, and Zn) on soil nitrification and determine toxicity thresholds via two cycles of sludge land application tests over 185 days. HMs inhibited gene abundance in their labile fractions, with the most affected being nitrite-oxidizing bacteria (NOB)-nxrB, followed by ammonia-oxidizing bacteria (AOB)-amoA, NOB-nxrA, and ammonia oxidizing archaea (AOA)-amoA. Toxicity thresholds for incremental labile fractions of HMs (in mg/kg) were determined as 0.35 for Cd, 21.73 for Cu, and 84.04 for Zn. Additionally, AOB, as the core nitrifiers, significantly correlated (P < 0.05) with ammonia nitrogen, soil organic matter, total phosphorus, and total potassium, playing a pivotal role in maintaining intricate interactions within HMs-spiked sludge-treated soil systems. The acute toxicity effects of HMs on potential ammonia oxidation (PAO), measured by inhibition rates, were 77.04%, 73.63%, and 67.06% for Cd, Cu, and Zn, with labile fractions contributing 33.79%, 40.19%, and 28.37%, respectively. Long-term sludge land application revealed chronic toxicity of HMs to PAO through the reshaping of ammonia-oxidizing microorganisms, particularly Cu and Zn. These findings provide insights into HM toxicity thresholds and their impact on nitrification, supporting sustainable sludge land management.
- Labile heavy metals,
- Sludge land management,
- Nitrifiers,
- Ammonia oxidation capacity,
- Dynamic effects
1. [1]
  X. Li, S. Yuan, C. Cai, et al., Environ. Pollut. 341 (2024) 122907.
2. [2]
  A. Pathak, M.G. Dastidar, T.R. Sreekrishnan, J. Environ. Manag. 90 (2009) 2343–2353.
3. [3]
  K. Qin, L. Wei, J. Li, et al., Chin. Chem. Lett. 31 (2020) 2603–2613.
4. [4]
  J. Zhang, Z. Liu, B. Tian, et al., J. Hazard. Mater. 441 (2023) 129891.
5. [5]
  V. Kapoor, X. Li, M. Elk, et al., Environ. Sci. Technol. 49 (2015) 13454–13462. doi: 10.1021/acs.est.5b02748
6. [6]
  L. Wei, F. Zhu, Q. Li, et al., Environ. Int. 144 (2020) 106093.
7. [7]
  H. Yesil, R. Molaey, B. Calli, A.E. Tugtas, Water Res. 201 (2021) 117303.
8. [8]
  Y. Xu, Y. Feng, Pol. J. Environ. Stud. 25 (2016) 405–412. doi: 10.15244/pjoes/60861
9. [9]
  J. Jia, J. Bai, R. Xiao, et al., Sci. Total Environ. 807 (2022) 151725.
10. [10]
  L. Li, Y. Ling, H. Wang, et al., Chin. Chem. Lett. 31 (2020) 28–38. doi: 10.5703/educationculture.36.1.0028
11. [11]
  M. Kuypers, H.K. Marchant, B. Kartal, Nat. Rev. Microbiol. 16 (2018) 263–276. doi: 10.1038/nrmicro.2018.9
12. [12]
  B. Xi, H. Yu, Y. Li, et al., J. Hazard. Mater. 403 (2021) 123853.
13. [13]
  S. Tang, Y. Rao, S. Huang, et al., J. Environ. Manag. 326 (2023) 116641.
14. [14]
  S. Cela, M.E. Sumner, Commun. Soil Sci. Plant Anal. 33 (2002) 19–30.
15. [15]
  L. Lu, C. Chen, T. Ke, et al., Sci. Total Environ. 830 (2022) 154732.
16. [16]
  H. He, H. Liu, T. Shen, et al., Geoderma. 321 (2018) 141–150.
17. [17]
  Y. Wang, X. Zeng, Y. Zhang, et al. J. Environ. Sci. 127 (2023) 15–29.
18. [18]
  J. Li, Y. Huang, Y. Hu, et al., J. Environ. Sci. 44 (2016) 131–140. doi: 10.26599/jgse.2016.9280016
19. [19]
  C. Yanez, J. Verdejo, H. Moya, et al., Chemosphere. 300 (2022) 134517.
20. [20]
  X. Zhang, X. Zhang, L. Li, et al., Environ. Res. 204 (2022) 111941.
21. [21]
  R. Dhanker, S. Chaudhary, S. Goyal, V.K. Garg, Environ. Technol. Innov. 23 (2021) 101642.
22. [22]
  M.Z. Hashmi, Naveedullah, H. Shen, et al., Environ. Int. 64 (2014) 28–39.
23. [23]
  S. Ruyters, J. Mertens, D. Springael, E. Smolders, Soil Biol. Biochem. 42 (2010) 766–772.
24. [24]
  J. Song, Q. Shen, L. Wang, et al., Environ. Pollut. 243 (2018) 510–518. doi: 10.3390/f9090510
25. [25]
  H. Zhao, J. Lin, X. Wang, et al., Environ. Sci. Technol. 55 (2021) 14305–14315. doi: 10.1021/acs.est.1c04409
26. [26]
  A.E. Taylor, L.H. Zeglin, T.A. Wanzek, D.D. Myrold, P.J. Bottomley, ISME J. 6 (2012) 2024–2032. doi: 10.1038/ismej.2012.51
27. [27]
  Y. Geng, C. Zhang, Y. Zhang, et al., Sci. Pollut. Res. 28 (2021) 29146–29156. doi: 10.1007/s11356-021-12762-8
28. [28]
  S. Qin, H. Liu, Z. Nie, et al., Pedosphere. 30 (2020) 168–180.
29. [29]
  J. Li, H. Ma, H. Yu, et al., J. Hazard. Mater. 466 (2024) 133552.
30. [30]
  A. Tessier, P.G.C. Campbell, M. Bisson, Anal. Chem. 51 (1979) 844–851. doi: 10.1021/ac50043a017
31. [31]
  J. Li, H. Yang, K. Qin, et al., J. Hazard. Mater. 423 (2022) 126994.
32. [32]
  Z. Cheng, J. Shi, Y. He, L. Wu, J. Xu, J. Hazard. Mater. 426 (2022) 128095.
33. [33]
  S. Cipullo, B. Snapir, G. Prpich, P. Campo, F. Coulon, Chemosphere 215 (2019) 388–395.
34. [34]
  T. Zeng, Y. Liang, Q. Dai, et al., Chin. Chem. Lett. 33 (2022) 5184–5188.
35. [35]
  B. Wang, B. Huang, Y.B. Qi, W.Y. Hu, W.X. Sun, Chin. Chem. Lett. 23 (2012) 1287–1290.
36. [36]
  G. Liu, Z. Yu, X. Liu, et al., J. Chem. 2020 (2020) 1–10.
37. [37]
  J. Yang, Z. Guo, L. Jiang, et al., Ecotoxicol. Environ. Saf. 239 (2022) 113617.
38. [38]
  G. Xueyuan, L.J. Evans, S.J. Barabash, Geochim. Cosmochim. Acta 74 (2010) 5718–5728.
39. [39]
  H.B. Bradl, J. Colloid Interface Sci. 277 (2004) 1–18.
40. [40]
  L. Mouni, L. Belkhiri, A. Bouzaza, J. Bollinger, Environ. Earth Sci. 75 (2016) 1–8.
41. [41]
  H. Liu, Waste Manag. 56 (2016) 575–583.
42. [42]
  M.M. Ali, A. Khanom, K. Nahar, et al., Environ. Contam. Toxicol. 106 (2021) 707–713. doi: 10.1007/s00128-021-03112-y
43. [43]
  D. Fan, S. Wang, Y. Guo, et al., Sci. Total Environ. 757 (2021) 143771.
44. [44]
  P. Gong, S.D. Siciliano, S. Srivastava, C.W. Greer, G.I. Sunahara, Hum. Ecol. Risk Assess. 8 (2002) 1067–1081. doi: 10.1080/1080-700291905828
45. [45]
  Q. Wu, K. Huang, H. Sun, et al., J. Hazard. Mater. 343 (2018) 166–175.
46. [46]
  T.S. Radniecki, R.L. Ely, Biotechnol. Bioeng. 99 (2008) 1085–1095. doi: 10.1002/bit.21672
47. [47]
  J.E. Coleman, Biochem. Biophys. Res. Commun. 60 (1974) 641.
48. [48]
  T.S. Radniecki, L. Semprini, M.E. Dolan, Biotechnol. Bioeng. 104 (2009) 1004–1011. doi: 10.1002/bit.22454
49. [49]
  X. Zhao, J. Huang, J. Lu, Y. Sun, Ecotoxicol. Environ. Saf. 170 (2019) 218–226.
50. [50]
  W. Longhua, C. Miaomiao, L. Zhu, et al., J. Soils Sediments 12 (2012) 531–541.
51. [51]
  C.D.N.A. Tsadilas, T. Matsi, N. Barbayiannis, D. Dimoyiannis, Commun. Soil Sci. Plant Anal. 26 (1995) 2603–2619. doi: 10.1080/00103629509369471
52. [52]
  A. Charlton, R. Sakrabani, S. Tyrrel, C.M. Rivas, S.P. McGrath, et al., Environ. Pollut. 219 (2016) 1021–1035.
53. [53]
  Y. Xing, Y.X. Si, C. Hong, Y. Li, Arch. Environ. Contam. Toxicol. 69 (2015) 20–31. doi: 10.1007/s00244-015-0144-9
54. [54]
  B. Shen, X. Wang, Y. Zhang, et al., Sci. Total Environ. 711 (2020) 135229.
55. [55]
  J. Li, Y. Zheng, Y. Liu, et al., Microb. Ecol. 67 (2014) 931–941. doi: 10.1007/s00248-014-0391-8
56. [56]
  S. Cela, M.E. Sumner, Water Air Soil Pollut. 141 (2002) 91–104.
57. [57]
  J. Mertens, K. Broos, S.A. Wakelin, et al., ISME J. 3 (2009) 916–923. doi: 10.1038/ismej.2009.39
58. [58]
  W. Qin, S.P. Wei, Y. Zheng, et al., Nat. Microbiol. 9 (2024) 524–536. doi: 10.1038/s41564-023-01593-7
59. [59]
  A. Ginawi, L. Wang, H. Wang, B. Yu, Y. Yunjun, PeerJ 8 (2020) e8256. doi: 10.7717/peerj.8256
1. [1]
  Jiahui Li , Qiao Shi , Ying Xue , Mingde Zheng , Long Liu , Tuoyu Geng , Daoqing Gong , Minmeng Zhao . The effects of in ovo feeding of selenized glucose on liver selenium concentration and antioxidant capacity in neonatal broilers. Chinese Chemical Letters, 2024, 35(6): 109239-. doi: 10.1016/j.cclet.2023.109239
2. [2]
  Yanling Yang , Zhenfa Ding , Huimin Wang , Jianhui Li , Yanping Zheng , Hongquan Guo , Li Zhang , Bing Yang , Qingqing Gu , Haifeng Xiong , Yifei Sun . Dynamic tracking of exsolved PdPt alloy/perovskite catalyst for efficient lean methane oxidation. Chinese Chemical Letters, 2024, 35(4): 108585-. doi: 10.1016/j.cclet.2023.108585
3. [3]
  Shili Wang , Mamitiana Roger Razanajatovo , Xuedong Du , Shunli Wan , Xin He , Qiuming Peng , Qingrui Zhang . Recent advances on decomplexation mechanisms of heavy metal complexes in persulfate-based advanced oxidation processes. Chinese Chemical Letters, 2024, 35(6): 109140-. doi: 10.1016/j.cclet.2023.109140
4. [4]
  Xiaoqiang Wang , Fangyuan Zhou , Yue Liu , Zhongbiao Wu . CePO₄ supported Cr catalyst with superior sulfur tolerance for selective catalytic oxidation of ammonia. Chinese Chemical Letters, 2025, 36(7): 110420-. doi: 10.1016/j.cclet.2024.110420
5. [5]
  Huixin Chen , Chen Zhao , Hongjun Yue , Guiming Zhong , Xiang Han , Liang Yin , Ding Chen . Unraveling the reaction mechanism of high reversible capacity CuP₂/C anode with native oxidation PO_x component for sodium-ion batteries. Chinese Chemical Letters, 2025, 36(1): 109650-. doi: 10.1016/j.cclet.2024.109650
6. [6]
  Rui Wang , Yang Liang , Julius Rebek Jr. , Yang Yu . Stabilization and detection of labile reaction intermediates in supramolecular containers. Chinese Chemical Letters, 2024, 35(6): 109228-. doi: 10.1016/j.cclet.2023.109228
7. [7]
  Zhiwen Li , Jingjing Zhang , Gao Li . Dynamic assembly of chiral golden knots. Chinese Journal of Structural Chemistry, 2024, 43(7): 100300-100300. doi: 10.1016/j.cjsc.2024.100300
8. [8]
  Hang Wang , Qi Wang , Chuan-De Wu . Continuous synthesis of ammonia. Chinese Journal of Structural Chemistry, 2025, 44(3): 100437-100437. doi: 10.1016/j.cjsc.2024.100437
9. [9]
  Yaxian Liang , Qingyi Li , Liwei Hu , Ruohan Zhai , Fan Liu , Lin Tan , Xiaofei Wang , Huixu Xie . Environmentally friendly polylysine gauze dressing for an innovative antimicrobial approach to infected wound management. Chinese Chemical Letters, 2024, 35(10): 109459-. doi: 10.1016/j.cclet.2023.109459
10. [10]
  Jiayi Lu , Yizhang Li , Hao Jiang , Zhiwen Zhu , Fengru Zheng , Qiang Sun . Preparing sub-monolayer metals with continuous coverage spread for high-throughput growth of metal-organic frameworks. Chinese Chemical Letters, 2025, 36(3): 110394-. doi: 10.1016/j.cclet.2024.110394
11. [11]
  Pei Cao , Yilan Wang , Lejian Yu , Miao Wang , Liming Zhao , Xu Hou . Dynamic asymmetric mechanical responsive carbon nanotube fiber for ionic logic gate. Chinese Chemical Letters, 2024, 35(6): 109421-. doi: 10.1016/j.cclet.2023.109421
12. [12]
  Guoliang Liu , Zhiqiang Liu , Anmin Zheng . Modulation of zeolite surface realizes dynamic copper species redispersion. Chinese Journal of Structural Chemistry, 2024, 43(6): 100308-100308. doi: 10.1016/j.cjsc.2024.100308
13. [13]
  Hang Meng , Bicheng Zhu , Ruolun Sun , Zixuan Liu , Shaowen Cao , Kan Zhang , Jiaguo Yu , Jingsan Xu . Dynamic photoluminescence switching of carbon nitride thin films for anticounterfeiting and encryption. Chinese Journal of Structural Chemistry, 2024, 43(10): 100410-100410. doi: 10.1016/j.cjsc.2024.100410
14. [14]
  Yujuan Zhou , Kecheng Jie . Conformationally adaptive metal–organic cages for dynamic guest encapsulation. Chinese Chemical Letters, 2025, 36(6): 111007-. doi: 10.1016/j.cclet.2025.111007
15. [15]
  Kongchuan Wu , Dandan Lu , Jianbin Lin , Ting-Bin Wen , Wei Hao , Kai Tan , Hui-Jun Zhang . Elucidating ligand effects in rhodium(Ⅲ)-catalyzed arene–alkene coupling reactions. Chinese Chemical Letters, 2024, 35(5): 108906-. doi: 10.1016/j.cclet.2023.108906
16. [16]
  Yue WANG , Zhizhi GU , Jingyi DONG , Jie ZHU , Cunguang LIU , Guohan LI , Meichen LU , Jian HAN , Shengnan CAO , Wei WANG . Effects of kelp-derived carbon dots on embryonic development of zebrafish. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1209-1217. doi: 10.11862/CJIC.20230423
17. [17]
  Qiang Fu , Shouhong Sun , Kangzhi Lu , Ning Li , Zhanhua Dong . Boron-doped carbon dots: Doping strategies, performance effects, and applications. Chinese Chemical Letters, 2024, 35(7): 109136-. doi: 10.1016/j.cclet.2023.109136
18. [18]
  Luyao Lu , Chen Zhu , Fei Li , Pu Wang , Xi Kang , Yong Pei , Manzhou Zhu . Ligand effects on geometric structures and catalytic activities of atomically precise copper nanoclusters. Chinese Journal of Structural Chemistry, 2024, 43(10): 100411-100411. doi: 10.1016/j.cjsc.2024.100411
19. [19]
  Rui HUANG , Shengjie LIU , Qingyuan WU , Nanfeng ZHENG . Enhanced selectivity of catalytic hydrogenation of halogenated nitroaromatics by interfacial effects. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 201-212. doi: 10.11862/CJIC.20240356
20. [20]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(4)
HTML views(1)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142