Citation: Yanhua Chen, Xian Ding, Jun Zhou, Zhaoying Wang, Yunhai Bo, Ying Hu, Qingce Zang, Jing Xu, Ruiping Zhang, Jiuming He, Fen Yang, Zeper Abliz. Plasma metabolomics combined with mass spectrometry imaging reveals crosstalk between tumor and plasma in gastric cancer genesis and metastasis[J]. Chinese Chemical Letters, ;2025, 36(1): 110351. doi: 10.1016/j.cclet.2024.110351 shu

Plasma metabolomics combined with mass spectrometry imaging reveals crosstalk between tumor and plasma in gastric cancer genesis and metastasis

Yanhua Chen ^{a, e, f, 1} ,
Xian Ding ^{b, h, 1} ,
Jun Zhou ^{c, 1} ,
Zhaoying Wang ^{a, e} ,
Yunhai Bo ^d ,
Ying Hu ^g ,
Qingce Zang ^b ,
Jing Xu ^b ,
Ruiping Zhang ^b ,
Jiuming He ^b ,
Fen Yang ^{d, *,} ,
Zeper Abliz ^{b, *,}

a.
Key Laboratory of Mass Spectrometry Imaging and Metabolomics (State Ethnic Affairs Commission), Center for Imaging and Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China
b.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China
c.
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education/Beijing), Department of Gastrointestinal Oncology, Peking University Cancer Hospital & Institute, Beijing 100142, China
d.
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Center of Drug Clinical Trial, Peking University Cancer Hospital and Institute, Beijing 100142, China
e.
Beijing Engineering Research Center of Food Environment and Public Health, Minzu University of China, Beijing 100081, China
f.
Key laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Minzu University of China, Beijing 100081, China
g.
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of BioBank, Peking University Cancer Hospital and Institute, Beijing 100142, China
h.
Pharmacy Department of Beijing Chao–Yang Hospital, Capital Medical University, Beijing 100020, China

Received Date: 22 April 2024
Revised Date: 19 July 2024
Accepted Date: 20 August 2024
Available Online: 20 August 2024

Figures(4)

Gastric Carcinoma (GC) is a highly fatal malignant tumor with a poor prognosis. Its elevated mortality rates are primarily due to its proclivity for late-stage metastasis. Exploring the metabolic interactions between tumor microenvironment and the systemic bloodstream could help to clearly understand the mechanisms and identify precise biomarkers of tumor growth, proliferation, and metastasis. In this study, an integrative approach that combines plasma metabolomics with mass spectrometry imaging of tumor tissue was developed to investigate the global metabolic landscape of GC tumorigenesis and metastasis. The results showed that the oxidized glutathione to glutathione ratio (GSSH/GSH) became increased in non-distal metastatic GC (M0), which means an accumulation of oxidative stress in tumor tissues. Furthermore, it was found that the peroxidation of polyunsaturated fatty acids, such as 9,10-EpOMe, 9-HOTrE, etc., were accelerated in both plasma and tumor tissues of distal metastatic GC (M1). These changes were further confirmed the potential effect of CYP2E1 and GGT1 in metastatic potential of GC by mass spectrometry imaging (MSI) and immunohistochemistry (IHC). Collectively, our findings reveal the integrated multidimensional metabolomics approach is a clinical useful method to unravel the blood-tumor metabolic crosstalk, illuminate reprogrammed metabolic networks, and provide reliable circulating biomarkers.
- Metabolomics,
- Spatial-resolved metabolomics,
- Gastric cancer,
- Blood-tumor metabolic crosstalk,
- Lipid peroxidation
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