Citation: Yanfei Liu, Yaqin Hu, Yifu Tan, Qiwen Chen, Zhenbao Liu. Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition[J]. Chinese Chemical Letters, ;2025, 36(1): 110289. doi: 10.1016/j.cclet.2024.110289 shu

Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition

Yanfei Liu ^{b, 1} ,
Yaqin Hu ^{b, 1} ,
Yifu Tan ^a ,
Qiwen Chen ^b ,
Zhenbao Liu ^{a, c, *,}

a.
Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China
b.
Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
c.
Molecular Imaging Research Center of Central South University, Changsha 410008, China

zhenbaoliu@csu.edu.cn

Received Date: 13 June 2024
Revised Date: 18 July 2024
Accepted Date: 22 July 2024
Available Online: 23 July 2024

Figures(4)

Precise tumor targeting and therapy is a major trend in cancer treatment. Herein, we designed a tumor acidic microenvironment activatable drug loaded DNA nanostructure, in which, we made a clever use of the sequences of AS1411 and i-motif, which can partially hybridize, and designed a simple while robust DNA d-strand nanostructure, in which, i-motif sequence was designed to regulate the binding ability of the AS1411 aptamer to target tumor. In the normal physiological environment, i-motif inhibits the targeting ability of AS1411. In the acidic tumor microenvironment, i-motif forms a quadruplex conformation and dissociates from AS1411, restoring the targeting ability of AS1411. Only when acidic condition and tumor cell receptor are present, this nanostructure can be internalized by the tumor cells. Moreover, the structure change of this nanostructure can realize the release of loaded drug. This drug loaded A-I-Duplex DNA structure showed cancer cell and spheroid targeting and inhibition ability, which is promising in the clinical cancer therapy.
- Aptamer,
- i-motif,
- DNA nanostructure,
- Logic gate,
- Drug delivery system
1. [1]
  C. Tuerk, L. Gold, Science 249 (1990) 505–510. doi: 10.1126/science.2200121
2. [2]
  A. Pusuluri, V. Krishnan, V. Lensch, et al., Angew. Chem. Int. Ed. 58 (2019) 1437–1441. doi: 10.1002/anie.201812650
3. [3]
  H. Xiong, J. Yan, S. Cai, et al., Mol. Pharm. 17 (2020) 2882–2890. doi: 10.1021/acs.molpharmaceut.0c00335
4. [4]
  X. Sun, B. Liu, X. Chen, et al., J. Mater. Sci. Mater. Med. 30 (2019) 76. doi: 10.1007/s10856-019-6278-y
5. [5]
  Q. Ma, W. Qian, W. Tao, Y. Zhou, B. Xue, Drug Des. Devel. Ther. 13 (2019) 4021–4033. doi: 10.2147/dddt.s210949
6. [6]
  W.H. Chen, O. Karmi, B. Willner, R. Nechushtai, I. Willner, Sensors 19 (2019) 5260. doi: 10.3390/s19235260
7. [7]
  S. Cai, J. Yan, H. Xiong, et al., Int. J. Pharm. 590 (2020) 119948. doi: 10.1016/j.ijpharm.2020.119948
8. [8]
  N. Zhao, J. Coyne, L. Abune, et al., ACS Appl. Mater. Interfaces 12 (2020) 24599–24610. doi: 10.1021/acsami.0c05681
9. [9]
  J. Zou, M. Shi, X. Liu, et al., Anal. Chem. 91 (2019) 2425–2430. doi: 10.1021/acs.analchem.8b05204
10. [10]
  Y. Zhan, W. Ma, Y. Zhang, et al., ACS Appl. Mater. Interfaces 11 (2019) 15354–15369. doi: 10.1021/acsami.9b03449
11. [11]
  L. Li, J. Wang, H. Jiang, et al., Chin. Chem. Lett. 34 (2023) 107506. doi: 10.1016/j.cclet.2022.05.020
12. [12]
  Y. Zhang, Y. Liu, Y. Yang, et al., Chin. Chem. Lett. 34 (2023) 108102. doi: 10.1016/j.cclet.2022.108102
13. [13]
  L. Zhao, F. Ahmed, H. Xiong, Chin. Chem. Lett. 33 (2022) 4243–4247. doi: 10.1016/j.cclet.2022.02.048
14. [14]
  J. Jouha, F. Li, H. Xiong, Spectrochim. Acta A: Mol. Biomol. Spectrosc. 295 (2023) 122615. doi: 10.1016/j.saa.2023.122615
15. [15]
  A. Hulikova, P. Swietach, FASEB J. 28 (2014) 2762–2774. doi: 10.1096/fj.13-241752
16. [16]
  D. Hanahan, R.A. Weinberg, Cell 144 (2011) 646–674. doi: 10.1016/j.cell.2011.02.013
17. [17]
  D. Sen, W. Gilbert, Nature 334 (1988) 364–366. doi: 10.1038/334364a0
18. [18]
  P.J. Bates, D.A. Laber, D.M. Miller, S.D. Thomas, J.O. Trent, Exp. Mol. Pathol. 86 (2009) 151–164. doi: 10.1016/j.yexmp.2009.01.004
19. [19]
  X. Chen, D.M. Kube, M.J. Cooper, P.B. Davis, Mol. Ther. 16 (2008) 333–342. doi: 10.1038/sj.mt.6300365
20. [20]
  S. Soundararajan, W. Chen, E.K. Spicer, N. Courtenay-Luck, D.J. Fernandes, Cancer Res. 68 (2008) 2358–2365. doi: 10.1158/0008-5472.CAN-07-5723
21. [21]
  I. Mellman, R. Fuchs, A. Helenius, Annu. Rev. Biochem. 55 (1986) 663–700. doi: 10.1146/annurev.bi.55.070186.003311
22. [22]
  C. Nilsson, K. Kagedal, U. Johansson, K. Ollinger, Methods Cell Sci. 25 (2003) 185–194. doi: 10.1007/s11022-004-8228-3
23. [23]
  I.V. Nesterova, E.E. Nesterov, J. Am. Chem. Soc. 136 (2014) 8843–8846. doi: 10.1021/ja501859w
24. [24]
  J.N. Zadeh, C.D. Steenberg, J.S. Bois, et al., J. Comput. Chem. 32 (2011) 170–173. doi: 10.1002/jcc.21596
25. [25]
  J. Kim, C. Jo, W.G. Lim, et al., Adv. Mater. 30 (2018) 1707557. doi: 10.1002/adma.201707557
26. [26]
  S.H. Kim, K.R. Kim, D.R. Ahn, et al., ACS Nano 11 (2017) 9352–9359. doi: 10.1021/acsnano.7b04766
27. [27]
  K. Leung, K. Chakraborty, A. Saminathan, Y. Krishnan, Nat. Nanotechnol. 14 (2018) 176–183. doi: 10.1038/s41565-018-0318-5
28. [28]
  Y. Hu, Y. Wang, J. Yan, et al., Adv. Sci. 7 (2020) 2000557. doi: 10.1002/advs.202000557
29. [29]
  K. Haruta, N. Otaki, M. Nagamine, et al., Nucleic Acid Ther. 27 (2017) 36–44. doi: 10.1089/nat.2016.0627
30. [30]
  D. Thirunavukarasu, T. Chen, Z. Liu, N. Hongdilokkul, F.E. Romesberg, J. Am. Chem. Soc. 139 (2017) 2892–2895. doi: 10.1021/jacs.6b13132
31. [31]
  N.D. Abeydeera, M. Egli, N. Cox, et al., Nucleic Acids Res. 44 (2016) 8052–8064. doi: 10.1093/nar/gkw725
32. [32]
  C. Riccardi, C. Fabrega, S. Grijalvo, et al., J. Mater. Chem. B 6 (2018) 5368–5384. doi: 10.1039/c8tb01563e
33. [33]
  Z. Wang, L. Song, Q. Liu, et al., Angew. Chem. Int. Ed. 60 (2020) 2594–2598. doi: 10.1002/anie.201902359
34. [34]
  S. Zou, Y. Lei, W. Ma, et al., Analyst 145 (2020) 2562–2569. doi: 10.1039/d0an00101e
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