Citation: Qiuye Wang, Yabing Sun, Liangxue Lai, Haijing Cui, Yonglong Ye, Ming Yang, Weihao Zhu, Bo Yuan, Quanliang Mao, Wenzhi Ren, Aiguo Wu. MMP-9-responsive probe for fluorescence-magnetic resonance dual-mode imaging of hepatocellular carcinoma models with different metastatic capacities[J]. Chinese Chemical Letters, ;2025, 36(4): 110212. doi: 10.1016/j.cclet.2024.110212 shu

MMP-9-responsive probe for fluorescence-magnetic resonance dual-mode imaging of hepatocellular carcinoma models with different metastatic capacities

Qiuye Wang ^{a, 1} ,
Yabing Sun ^{a, b, 1} ,
Liangxue Lai ^{a, b} ,
Haijing Cui ^{a, b} ,
Yonglong Ye ^a ,
Ming Yang ^a ,
Weihao Zhu ^{a, b} ,
Bo Yuan ^{a, c} ,
Quanliang Mao ^d ,
Wenzhi Ren ^{a, b, c, *,} ,
Aiguo Wu ^{a, c, *,}

a.
Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Zhejiang International Cooperation Base of Biomedical Materials Technology and Application, Chinese Academy of Sciences (CAS) Key Laboratory of Magnetic Materials and Devices, Ningbo Cixi Institute of Biomedical Engineering, Zhejiang Engineering Research Center for Biomedical Materials, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, China
b.
Cixi Biomedical Research Institute, Wenzhou Medical University, Ningbo 315300, China
c.
Advanced Energy Science and Technology Guangdong Laboratory, Huizhou 516000, China
d.
Department of Radiology, First Affiliated Hospital of Ningbo University, Ningbo 315010, China

renwzh@nimte.ac.cn

aiguo@nimte.ac.cn

Received Date: 13 April 2024
Revised Date: 25 June 2024
Accepted Date: 3 July 2024
Available Online: 3 July 2024

Figures(5)

Visual assessment of tumor metastatic capacity is crucial for predicting hepatocellular carcinoma (HCC) prognosis and guiding clinical therapeutic approaches. In this study, we developed an enzyme-responsive probe based on the peptide GK10, which is selectively cleaved by matrix metalloproteinase-9 (MMP-9), a critical marker for metastasis in HCC. The GK10 peptide was conjugated with near-infrared fluorescent molecule IR783, fluorescent quencher black hole quencher 3 (BHQ3), and magnetic resonance (MR) contrast agent DOTA-Gd, forming the IR783-GK10-BHQ3-Gd probe. Upon MMP-9 cleavage of GK10, BHQ3 is released from the probe, thereby amplifying the previously quenched IR783 fluorescence signal. In vitro experiments demonstrate the probe’s impressive detection limit for MMP-9, as low as 1.84 ng/mL. Moreover, in vivo imaging results reveal that the probe can differentiate liver cancers with varying metastatic capacities. The fluorescence and MR imaging signal intensity of high metastatic HCC are approximately 1.2 times greater than that of low metastatic HCC. Thus, this engineered probe holds promise as a valuable tool for evaluating HCC metastatic capacity through fluorescence-MR dual-mode imaging.
- Hepatocellular carcinoma,
- Matrix metalloproteinase-9,
- Fluorescence imaging,
- Magneticresonance imaging,
- Förster resonance energy transfer
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