Citation: Yuwan Lu, Xiaodan Zhang, Yuming Huang. Dual-site Se/NC specific peroxidase-like nanozyme for highly sensitive methimazole detection[J]. Chinese Chemical Letters, ;2025, 36(4): 110129. doi: 10.1016/j.cclet.2024.110129 shu

Dual-site Se/NC specific peroxidase-like nanozyme for highly sensitive methimazole detection

Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China

ymhuang@swu.edu.cn

Received Date: 27 February 2024
Revised Date: 6 June 2024
Accepted Date: 14 June 2024
Available Online: 15 June 2024

Figures(6)

Tuning the nanozyme′s activity and specificity is very crucial for developing highly sensitive sensors for various applications. Herein, selenium-doped porous N-doped carbon skeletons (Se/NC) nanozymes with highly specific peroxidase-like activity were synthesized by a MOF-pyrolysis-doping protocol. Se doping adjusted the electronic structure of NC by introducing more vacancies, defective carbon and graphitic N, and endowed the resultant Se/NC enhanced charge transfer and substrate affinity. The Se/NC exhibited specific peroxidase-mimicking activity and could catalyze 3,3′,5,5′-tetramethylbenzidine oxidation by H₂O₂. Density functional theory (DFT) calculations and experimental trials indicated that both Se=O and C–Se–C species were the main active sites of Se/NC. The C–Se–C bond is the main catalytic active site endowing Se/NC with the property of nanozyme, while the Se=O bond effectively enhances its affinity to H₂O₂ and accelerate H₂O₂ dissociation. The Se/NC showed an approximately 185-fold increase in peroxidase-like activity compared to NC. Based on the inhibition of the peroxidase-like activity of Se/NC by methimazole, a colorimetric sensor was developed to achieve its sensitive detection with 2 nmol/L of limit of detection. It was successfully used for detecting methimazole in real samples. Current Se doping strategy simplifies the fabrication process of high performance specific nanozyme and promises great potential for environmental analysis.
- Se/NC peroxidase-like nanozyme,
- Dual active sites,
- Colorimetric assay,
- Methimazole,
- Environmental analysis
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