Citation: Lingjun Sha, Bing Bo, Jiayu Li, Qi Liu, Ya Cao, Jing Zhao. Precise assessment of lung cancer-derived exosomes based on dual-labelled membrane interface[J]. Chinese Chemical Letters, ;2025, 36(4): 110109. doi: 10.1016/j.cclet.2024.110109 shu

Precise assessment of lung cancer-derived exosomes based on dual-labelled membrane interface

Lingjun Sha ^{a, 1} ,
Bing Bo ^{b, 1,*,} ,
Jiayu Li ^b ,
Qi Liu ^a ,
Ya Cao ^{a, *,} ,
Jing Zhao ^{a, *,}

a.
Center for Molecular Recognition and Biosensing, Shanghai Engineering Research Center of Organ Repair, School of Life Sciences, Shanghai University, Shanghai 200444, China
b.
Department of Medical Oncology, Shanghai Pulmonary Hospital & Thoracic Cancer Institute, Tongji University School of Medicine, Shanghai 200433, China

1986042@qq.com

conezimint@shu.edu.cn

jingzhao@t.shu.edu.cn

Received Date: 25 March 2024
Revised Date: 30 April 2024
Accepted Date: 11 June 2024
Available Online: 12 June 2024

Figures(4)

Lung cancer-derived exosomes are a kind of valuable and clinically-predictable biomarkers for lung cancer, but they have the limitations in individual differences when being applied in liquid biopsy. To improve their application value and accuracy in clinical diagnosis, a dual-labelled electrochemical method is herein reported for precise assessment of lung cancer-derived exosomes. To do so, two probes are prepared for the dual labeling of exosome membrane to run DNA assembly reactions: One is modified with cholesterol and can insert into exosome membrane through hydrophobic interaction; another one is linked with programmed death ligand-1 (PD-L1) antibody and can bind to exosome surface-expressing PD-L1 via specific immunoreaction. Quantum dots-tagged signal strands are used to collect respective DNA products, and produce stripping signals corresponding to the amounts of total exosome and surface-expressing PD-L1, respectively. A wide linear relationship is established for the quantitative determination of lung cancer-derived exosomes in the range from 10³ to 10¹⁰ particles/mL, whereas the ratiometric value of the two stripping signals is proven to have a better diagnostic use in screening and staging of lung cancer when being applied to clinical samples. Therefore, our method might provide a new insight into precise diagnosis of lung cancer, and offer sufficient information to reflect the biomarker level and guide the personalized treatment level even at an early stage in clinic.
- Lung cancer-derived exosome,
- Programmed death ligand-1,
- DNA assembly reaction,
- Ratiometric signal,
- Electrochemical analysis
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沈阳化工大学材料科学与工程学院沈阳 110142