Citation: Shaobin He, Xiaoyun Guo, Qionghua Zheng, Huanran Shen, Yuan Xu, Fenglin Lin, Jincheng Chen, Haohua Deng, Yiming Zeng, Wei Chen. Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay[J]. Chinese Chemical Letters, ;2025, 36(4): 110096. doi: 10.1016/j.cclet.2024.110096 shu

Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay

Shaobin He ^{a, b, e, 1} ,
Xiaoyun Guo ^{a, 1} ,
Qionghua Zheng ^a ,
Huanran Shen ^a ,
Yuan Xu ^c ,
Fenglin Lin ^d ,
Jincheng Chen ^b ,
Haohua Deng ^a ,
Yiming Zeng ^{c, e, *,} ,
Wei Chen ^{a, *,}

a.
Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou 350004, China
b.
Laboratory of Clinical Pharmacy, Department of Pharmacy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China
c.
Department of Pulmonary and Critical Care Medicine, The Second Affiliated Hospital of Fujian Medical University, Center of Respiratory Medicine of Fujian Province, Quanzhou 362000, China
d.
Department of Pharmacy, Quanzhou Women’s and Children’s Hospital, Quanzhou 362000, China
e.
Fujian Key Laboratory of Lung Stem Cells, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, China

^**

zeng_yi_ming@126.com

chenandhu@163.com

Received Date: 5 February 2024
Revised Date: 28 April 2024
Accepted Date: 5 June 2024
Available Online: 5 June 2024

Figures(5)

Researchers have shown significant interest in modulating the peroxidase-like activity of nanozymes. Among these, bimetallic nanozymes have shown superior peroxidase-like activity over monometallic counterparts, offering enhanced performance and cost-efficiency in nanozyme designs. Herein, bimetallic nanozymes comprising nickel (Ni) and osmium (Os) incorporated into hyaluronate (HA) have been developed, resulting in HA-Ni_n/Os nanoclusters. Subsequently, comprehensive characterizations have been conducted. Further investigation has revealed that HA-Ni_n/Os efficiently catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation with hydrogen peroxide (H₂O₂), confirming its peroxidase-like behavior and role as a nanozyme. Impressively, HA-Ni₂/Os (Ni/Os = 2:1) displays heightened substrate affinity, accelerated reaction rates, enhanced hydroxyl radical production in acidic conditions, and exhibits activity unit of 1224 U/mg, representing more than two-fold increase compared to non-Ni-supported Os nanozyme. Theoretical calculations indicate that Ni support enhances the peroxidase-like process of Os nanozyme by improving H₂O₂ adsorption and TMB oxidation. Crucially, the support of Ni does not significantly alter the other enzyme-like activities of Os nanozymes, thereby enabling Ni to selectively enhance their peroxidase-like activity. In terms of application, the peroxidase-like ability of HA-Ni₂/Os, facilitated by HA's carboxyl groups enabling crosslinking, proves effective in a squamous carcinoma antigen immunoassay. Moreover, HA-Ni₂/Os exhibit reliable stability, promising as a peroxidase substitute. This work underscores the advantages of incorporating Ni into Os, specifically enhancing peroxidase-like activity, highlighting the potential of Os bimetallic nanozymes for peroxidase-based applications.
- Nanozyme,
- Osmium,
- Nickel,
- Bimetallic,
- Peroxidase-like activity,
- Immunoassay
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