Citation: Keqiang Shi, Xiujuan Hong, Dongyan Xu, Tao Pan, Huiwen Wang, Hongru Feng, Cheng Guo, Yuanjiang Pan. Analysis of RNA modifications in peripheral white blood cells from breast cancer patients by mass spectrometry[J]. Chinese Chemical Letters, ;2025, 36(3): 110079. doi: 10.1016/j.cclet.2024.110079 shu

Analysis of RNA modifications in peripheral white blood cells from breast cancer patients by mass spectrometry

Keqiang Shi ^{a, b, c} ,
Xiujuan Hong ^{a, c} ,
Dongyan Xu ^{a, c} ,
Tao Pan ^d ,
Huiwen Wang ^e ,
Hongru Feng ^b ,
Cheng Guo ^{a, c, *,} ,
Yuanjiang Pan ^{b, *,}

a.
Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
b.
Department of Chemistry, Zhejiang University, Hangzhou 310058, China
c.
Cancer Center, Zhejiang University, Hangzhou 310058, China
d.
Department of Breast Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China
e.
Analysis Center of Agrobiology and Environmental Sciences, Zhejiang University, Hangzhou 310058, China

cheng_guo@zju.edu.cn

panyuanjiang@zju.edu.cn

Received Date: 23 January 2024
Revised Date: 15 April 2024
Accepted Date: 31 May 2024
Available Online: 1 June 2024

Figures(5)

RNA modifications play vital regulatory roles in biological systems. Dysregulated RNA modifications themselves or their regulators are associated with various diseases, including cancers and immune related diseases. However, to the best of our knowledge, RNA modifications in peripheral white blood cells (immune cells) have not been systematically investigated before. Here we utilized hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS) for the quantification of 19 chemical modifications in total RNA and 17 chemical modifications in small RNA in peripheral white blood cells from breast cancer patients and healthy controls. We found out 13 RNA modifications were up-regulated in total RNA samples of breast cancer patients. For small RNA samples, only N⁶-methyladenosine (m⁶A) was down-regulated in breast cancer patients (P < 0.0001). Receiver operating characteristic (ROC) curves analysis showed that N⁴-acetylcytidine (ac⁴C) in total RNA had an area under curve (AUC) value of 0.833, and m⁶A in small RNA had an AUC value of 0.994. Our results further illustrated that RNA modifications may play vital roles in immune cell biology of breast cancer, and may act as novel biomarkers for the diagnosis of breast cancer.
- RNA modification,
- Mass spectrometry,
- Breast cancer,
- Immune cell,
- Biomarker
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