Citation: Gaojian Yang, Zhiyang Li, Rabia Usman, Zhu Chen, Yuan Liu, Song Li, Hui Chen, Yan Deng, Yile Fang, Nongyue He. DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer[J]. Chinese Chemical Letters, ;2025, 36(2): 109930. doi: 10.1016/j.cclet.2024.109930 shu

DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer

Gaojian Yang ^{a, 1} ,
Zhiyang Li ^{c, 1} ,
Rabia Usman ^a ,
Zhu Chen ^{b, d, e} ,
Yuan Liu ^{d, e, *,} ,
Song Li ^{d, e} ,
Hui Chen ^{d, e} ,
Yan Deng ^{d, e, *,} ,
Yile Fang ^{a, *,} ,
Nongyue He ^{a, b, *,}

a.
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
c.
Department of Clinical Laboratory, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China
d.
Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang 421001, China
e.
Institute for Future Sciences, University of South China, Changsha 410000, China

Received Date: 20 February 2024
Revised Date: 19 April 2024
Accepted Date: 27 April 2024
Available Online: 29 April 2024

Figures(4)

The extracellular vesicles show great potential as a noninvasive biomarker for the early detection of cancer. Hence, there is an urgent requirement to create biosensors that are time-saving, simple, and easily scalable in order to accomplish rapid, sensitive, and quantitative detection of extracellular vesicles. In this study, we present a self-propelled DNA walker powered by endonuclease Nt.BbvCI, which enables the development of a "signal on" sensing platform for the rapid and highly sensitive detection of extracellular vesicles. The DNA motor employed tracks made of streptavidin magnetic beads, which consisted of substrate strands labeled with fluorescein and motor strands locked by aptamers. The aptamer recognition of the target protein on extracellular vesicles unlocked the motor strand, initiating the DNA motor process. After replacing the optimal buffer solution containing the endonuclease Nt.BbvCI, the motor strands autonomously moved along the streptavidin magnetic beads track, continuously releasing fluorescent molecules and producing detectable fluorescence signals. Under optimal conditions, the detection range was from 2×10⁴ particles/mL to 2×10⁹ particles/mL, with a detection limit of 2.9×10³ particles/mL, demonstrating excellent selectivity. This method has demonstrated good selectivity in different tumor-derived extracellular vesicles and performs well in complex biological samples. The ability to effectively analyze surface proteins of extracellular vesicles in a short period of time gives our DNA walker a tremendous potential for developing simple and cost-effective clinical diagnostic devices.
- Extracellular vesicles,
- Aptamer,
- Streptavidin magnetic beads,
- Nt.BbvCI,
- Detection
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