Citation: Xiongbo Song, Jinwen Xiao, Juan Wu, Li Sun, Long Chen. Decellularized amniotic membrane promotes the anti-inflammatory response of macrophages via PI3K/AKT/HIF-1α pathway[J]. Chinese Chemical Letters, ;2025, 36(1): 109844. doi: 10.1016/j.cclet.2024.109844 shu

Decellularized amniotic membrane promotes the anti-inflammatory response of macrophages via PI3K/AKT/HIF-1α pathway

Xiongbo Song ^{a, b} ,
Jinwen Xiao ^{a, b} ,
Juan Wu ^c ,
Li Sun ^{a, b} ,
Long Chen ^{a, b, *,}

a.
Department of Orthopedics, Guizhou Provincial People's Hospital, Guiyang 550000, China
b.
The Lab of Tissue Engineering and Translational Medicine, College of Medicine, Guizhou University, Guiyang 550000, China
c.
Wuhan Kangchuang Biotechnology, Wuhan 430074, China

longchen@gzu.edu.cn

Received Date: 3 February 2024
Revised Date: 25 March 2024
Accepted Date: 29 March 2024
Available Online: 29 March 2024

Figures(3)

Macrophages undergo dynamic transitions between M1 and M2 states, exerting profound influences on both inflammatory and regenerative processes. The biocompatible and wound-healing properties of decellularized amniotic membrane (dAM) make it a subject of exploration for its potential impact on the anti-inflammatory response of macrophages. Experimental findings unequivocally demonstrate that dAM promotes anti-inflammatory M2 polarization of macrophage, with its cytokine-rich content posited as a potential mediator. The application of RNA sequencing unveils differential gene expression, implicating the hypoxia inducible factor-1α (HIF-1α) signaling pathway in this intricate interplay. Subsequent investigation further demonstrates that dAM facilitates anti-inflammatory M2 polarization of macrophage through the upregulation of epidermal growth factor (EGF), which, in turn, activates the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) pathway and stabilizes HIF-1α. This cascade results in a noteworthy augmentation of anti-inflammatory gene expression. This study significantly contributes to advancing our comprehension of dAM's immunomodulatory role in tissue repair, thereby suggesting promising therapeutic potential.
- Macrophage,
- Decellularized amniotic membrane,
- Anti-inflammatory response,
- Hypoxia inducible factor-1α,
- Epidermal growth factor
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沈阳化工大学材料科学与工程学院沈阳 110142