Citation: Guang-Xu Duan, Queting Chen, Rui-Rui Shao, Hui-Huang Sun, Tong Yuan, Dong-Hao Zhang. Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO₂ nano-membrane for enhancing catalytic performance[J]. Chinese Chemical Letters, ;2025, 36(2): 109751. doi: 10.1016/j.cclet.2024.109751 shu

Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO₂ nano-membrane for enhancing catalytic performance

Guang-Xu Duan ^{a, 1} ,
Queting Chen ^{d, 1} ,
Rui-Rui Shao ^a ,
Hui-Huang Sun ^a ,
Tong Yuan ^a ,
Dong-Hao Zhang ^{a, b, c, *,}

a.
College of Pharmaceutical Science, Hebei University, Baoding 071002, China
b.
Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Science, Hebei University, Baoding 071002, China
c.
Institute of Life Science and Green Development, Hebei University, Baoding 071002, China
d.
Affiliated Hospital of Hebei University, Baoding 071000, China

donghaozhang1@163.com

dhzhang@hbu.edu.cn

Received Date: 11 January 2024
Revised Date: 1 March 2024
Accepted Date: 6 March 2024
Available Online: 8 March 2024

Figures(4)

The preparation of immobilized enzyme with excellent performance is one of the difficulties that restrict the application of enzyme catalysis technology. Here, Candida rugosa lipase (CRL) was firstly adsorbed on the surface of magnetic zeolitic imidazolate framework-8 (ZIF-8) nanospheres, which was further encapsulated with a mesoporous SiO₂ nano-membrane formed by tetraethyl orthosilicate (TEOS) polycondensation. Consequently, lipase could be firmly immobilized on carrier surface by physical binding rather than chemical binding, which did not damage the active conformation of enzyme. There were mesopores on the silica nano-membrane, which could improve the accessibility of enzyme and its apparent catalytic activity. Moreover, silica membrane encapsulation could also improve the stability of enzyme, suggesting an effective enzyme immobilization strategy. It showed that TEOS amount and the encapsulation time had significant effects on the thickness of silica membrane and the enzyme activity. The analysis in enzyme activity and protein secondary structure showed that lipase encapsulated in silica membrane retained the active conformation to the greatest extent. Compared with the adsorbed lipase, the encapsulated lipase increased its thermostability by 3 times and resistance to chemical denaturants by 7 times. The relative enzyme activity remained around 80% after 8 repetitions, while the adsorbed lipase only remained at 7.3%.
- Enzyme immobilization,
- ZIF-8,
- Encapsulation,
- Mesoporous silica membrane,
- Lipase activity
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沈阳化工大学材料科学与工程学院沈阳 110142

Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO2 nano-membrane for enhancing catalytic performance

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通讯作者: 陈斌, bchen63@163.com

Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO₂ nano-membrane for enhancing catalytic performance