Citation: Fangping Yang, Jin Shi, Yuansong Wei, Qing Gao, Jingrui Shen, Lichen Yin, Haoyu Tang. Mixed-charge glycopolypeptides as antibacterial coatings with long-term activity[J]. Chinese Chemical Letters, ;2025, 36(2): 109746. doi: 10.1016/j.cclet.2024.109746 shu

Mixed-charge glycopolypeptides as antibacterial coatings with long-term activity

Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215123, China

hytang@suda.edu.cn

Received Date: 10 January 2024
Revised Date: 29 February 2024
Accepted Date: 5 March 2024
Available Online: 8 March 2024

Figures(6)

Planktonic bacteria adhere and subsequently form biofilms on implantable medical devices can cause severe infections that have become the major types of hospital-acquired infections. Traditional coatings for the implants are frequently lack of long-term antifouling and bactericidal activities. It is still a big challenge to simultaneously improve the antifouling and bactericidal activities of the coatings. Herein, we report that mixed-charge glycopolypeptide coatings are of long-term antibacterial activities to efficiently inhibit the biofilm growth. The glycosylation of mixed-charge polypeptides has led to a significant improvement of both antifouling and bactericidal activities. The cooperative effect of the saccharide residues and mixed-charge residues improved the resistance of the polypeptide coatings against protein adsorption. The saccharide and L-glutamic acid (E) residues collectively enhanced the bacterial membrane-disruption of cationic L-lysine (K) residues, leading to potent bactericidal activity. Meanwhile, the glycopolypeptide coatings showed superior biocompatibility, long-term antibiofilm and anti-infection properties in two types of mouse subcutaneous infection models and one type of mouse urinary tract infection model. This work provides a new strategy to achieve antibacterial coatings with long-term activities for preventing implantable medical device associated infections.
- Antibacterial coating,
- Glycopolypeptide,
- Bactericidal activity,
- Fouling,
- Biofilm
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