Citation: Ran Wu, Dongxu Jiang, Hao Hu, Chenyu Yang, Liang Qin, Lulu Chen, Zehui Hu, Hualei Xu, Jinrong Li, Haiqiang Liu, Hua Guo, Jinxiang Fu, Qichen Hao, Yijun Zhou, Jinchao Feng, Qiang Wang, Xiaodong Wang. 4-Aminoazobenzene: A novel negative ion matrix for enhanced MALDI tissue imaging of metabolites[J]. Chinese Chemical Letters, ;2024, 35(11): 109624. doi: 10.1016/j.cclet.2024.109624 shu

4-Aminoazobenzene: A novel negative ion matrix for enhanced MALDI tissue imaging of metabolites

Ran Wu ^{a, b, 1} ,
Dongxu Jiang ^{a, b, 1} ,
Hao Hu ^{a, b, 1} ,
Chenyu Yang ^{a, b, 1} ,
Liang Qin ^{a, b} ,
Lulu Chen ^{a, c} ,
Zehui Hu ^{d, e} ,
Hualei Xu ^{a, b} ,
Jinrong Li ^{a, b} ,
Haiqiang Liu ^{a, b} ,
Hua Guo ^{a, b} ,
Jinxiang Fu ^{a, b} ,
Qichen Hao ^{a, b} ,
Yijun Zhou ^b ,
Jinchao Feng ^b ,
Qiang Wang ^{d, e, *,} ,
Xiaodong Wang ^{a, b, *,}

a.
Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Beijing 100081, China
b.
College of Life and Environmental Sciences, Centre for Imaging & Systems Biology, Minzu University of China, Beijing 100081, China
c.
College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
d.
Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China
e.
Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou 310016, China

wangqiang1983@zju.edu.cn

Xiaodong@muc.edu.cn

Received Date: 15 September 2023
Revised Date: 12 January 2024
Accepted Date: 4 February 2024
Available Online: 13 February 2024

Figures(4)

Endogenous metabolites play key functions in many important physiological and biochemical processes. The comprehensive in situ detection and direct imaging of metabolites in bio-tissues by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is very important for understanding complex and diverse biological processes and has become an essential aspect of spatial omics. In this work, 4-aminoazobenzene (AAB) was successfully screened and optimized as a new negative ion (−)MALDI matrix to enhance the in situ detection and imaging of metabolites in tissues using MALDI-MSI. Obviously, AAB exhibited superior properties in terms of ultraviolet absorption, background ion interference, matrix morphology, and metabolite ionization efficiency. AAB was used for in situ detection and imaging of metabolites in rat brain and germinating Chinese yew seed tissue sections, where 264 and 339 metabolite ion signals were successfully detected and imaged using (-)MALDI-MS, respectively. In addition, high-resolution imaging of mouse eyeball section using MALDI-timsTOF MSI with spatial resolution of up to 10 µm was successfully carried out, showing that AAB is an efficient (-)MALDI matrix for capturing high-resolution images of metabolites in biological tissue sections.
- MALDI-MSI,
- 4-Aminoazobenzene,
- Metabolites,
- In situ detection,
- Tissue imaging
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