Citation: Yan Liu, Yang Wang, Jiayi Zhu, Xuxian Su, Xudong Lin, Liang Xu, Xiwen Xing. Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells[J]. Chinese Chemical Letters, ;2024, 35(9): 109427. doi: 10.1016/j.cclet.2023.109427 shu

Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells

Yan Liu ^{a, 1} ,
Yang Wang ^{b, 1} ,
Jiayi Zhu ^{c, 1} ,
Xuxian Su ^b ,
Xudong Lin ^a ,
Liang Xu ^{b, *,} ,
Xiwen Xing ^{d, *,}

a.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China
b.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China
c.
Department of Cardiology, the first people's hospital of Foshan, Foshan 528000, China
d.
Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

xuliang33@mail.sysu.edu.cn

xingxiwen@jnu.edu.cn

Received Date: 21 September 2023
Revised Date: 10 December 2023
Accepted Date: 12 December 2023
Available Online: 22 December 2023

Figures(3)

The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms, which has been exploited for various applications in gene manipulation. Compared with the constantly active CRISPR/Cas9 function, conditional control of its activity can improve the performance of the system with reduced side effects and high spatiotemporal precision. The pH-responsive triplex RNA was successful used in CRISPR-derived RNA/trans-activating crRNA (crRNA/tracrRNA) of CRISPR/Cas9, thus affecting RNA/dead Cas9 (dCas9) complex to target DNA in vitro and in vivo. This design of triplex RNA opens a new window towards the broad involvement of eukaryotic cells for conditional control of CRISPR/Cas9 function.
- RNA triplex,
- pH-responsive,
- CRISPR/Cas9,
- Gene manipulation,
- Eukaryotic cells
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