Citation: Yan Liu, Yang Wang, Jiayi Zhu, Xuxian Su, Xudong Lin, Liang Xu, Xiwen Xing. Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells[J]. Chinese Chemical Letters, ;2024, 35(9): 109427. doi: 10.1016/j.cclet.2023.109427 shu

Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells

Yan Liu ^{a, 1} ,
Yang Wang ^{b, 1} ,
Jiayi Zhu ^{c, 1} ,
Xuxian Su ^b ,
Xudong Lin ^a ,
Liang Xu ^{b, *,} ,
Xiwen Xing ^{d, *,}

a.
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China
b.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou 510275, China
c.
Department of Cardiology, the first people's hospital of Foshan, Foshan 528000, China
d.
Department of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

xuliang33@mail.sysu.edu.cn

xingxiwen@jnu.edu.cn

Received Date: 21 September 2023
Revised Date: 10 December 2023
Accepted Date: 12 December 2023
Available Online: 22 December 2023

Figures(3)

The clustered regularly interspersed short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is an RNA-guided platform for highly efficient and specific genome targeting in diverse organisms, which has been exploited for various applications in gene manipulation. Compared with the constantly active CRISPR/Cas9 function, conditional control of its activity can improve the performance of the system with reduced side effects and high spatiotemporal precision. The pH-responsive triplex RNA was successful used in CRISPR-derived RNA/trans-activating crRNA (crRNA/tracrRNA) of CRISPR/Cas9, thus affecting RNA/dead Cas9 (dCas9) complex to target DNA in vitro and in vivo. This design of triplex RNA opens a new window towards the broad involvement of eukaryotic cells for conditional control of CRISPR/Cas9 function.
- RNA triplex,
- pH-responsive,
- CRISPR/Cas9,
- Gene manipulation,
- Eukaryotic cells
1. [1]
  L. Cong, F.A. Ran, D. Cox, et al., Science 339 (2013) 819–823. doi: 10.1126/science.1231143
2. [2]
  L.A. Gilbert, M.A. Horlbeck, B. Adamson, et al., Cell 159 (2014) 647–661. doi: 10.1016/j.cell.2014.09.029
3. [3]
  O.O. Abudayyeh, J.S. Gootenberg, B. Franklin, et al., Science 365 (2019) 382–386. doi: 10.1126/science.aax7063
4. [4]
  H. Wang, M. Nakamura, T.R. Abbott, et al., Science 365 (2019) 1301–1305. doi: 10.1126/science.aax7852
5. [5]
  H. Shivram, B.F. Cress, G.J. Knott, J.A. Doudna, Nat. Chem. Biol. 17 (2021) 10–19. doi: 10.1038/s41589-020-00700-7
6. [6]
  H. Deng, H. Xu, Y. Wang, et al., Nucleic Acids Res. 51 (2023) 4064–4077. doi: 10.1093/nar/gkad178
7. [7]
  R.S. Zou, Y. Liu, B. Wu, T. Ha, Mol. Cell 81 (2021) 1553–1565. doi: 10.1016/j.molcel.2021.02.007
8. [8]
  W. Zhou, W. Brown, A. Bardhan, et al., Angew. Chem. Int. Ed. 59 (2020) 8998–9003. doi: 10.1002/anie.201914575
9. [9]
  Y. Wang, C.Q. Wu, Q.L. Zhang, et al., CCS Chem. 6 (2024) 1338–1351. doi: 10.31635/ccschem.023.202303379
10. [10]
  B. Maji, C.L. Moore, B. Zetsche, et al., Nat. Chem. Biol. 13 (2017) 9–11. doi: 10.1038/nchembio.2224
11. [11]
  W. Brown, W. Zhou, A. Deiters, ChemBioChem 22 (2021) 63–72. doi: 10.1002/cbic.202000423
12. [12]
  L.F. Hu, Y.X. Li, J.Z. Wang, Y.T. Zhao, Y. Wang, Wires RNA 14 (2023) e1731. doi: 10.1002/wrna.1731
13. [13]
  S.R. Wang, L.Y. Wu, H.Y. Huang, et al., Nat. Commun. 11 (2020) 91. doi: 10.1038/s41467-019-13765-3
14. [14]
  Y. Wang, Y. Liu, F. Xie, J. Lin, L. Xu, Chem. Sci. 11 (2020) 11478–11484. doi: 10.1039/D0SC04343E
15. [15]
  D. Zhang, L. Liu, S. Jin, et al., J. Am. Chem. Soc. 144 (2022) 4487–4495. doi: 10.1021/jacs.1c12166
16. [16]
  J.R. Casey, S. Grinstein, J. Orlowski, Nat. Rev. Mol. Cell Biol. 11 (2010) 50–61. doi: 10.1038/nrm2820
17. [17]
  I. Koyuncu, E. Temiz, M. Durgun, et al., Int. J. Biol. Macromol. 201 (2022) 37–46. doi: 10.1016/j.ijbiomac.2021.12.190
18. [18]
  P. Swietach, Cancer Metast. Rev. 38 (2019) 5–15. doi: 10.1007/s10555-018-09778-x
19. [19]
  A. Idili, A. Vallee-Belisle, F. Ricci, J. Am. Chem. Soc. 136 (2014) 5836–5839. doi: 10.1021/ja500619w
20. [20]
  Y. Dong, Z. Yang, D. Liu, Accounts Chem. Res. 47 (2014) 1853–1860. doi: 10.1021/ar500073a
21. [21]
  I.A.P. Thompson, L. Zheng, M. Eisenstein, H.T. Soh, Nat. Commun. 11 (2020) 2946. doi: 10.1038/s41467-020-16808-2
22. [22]
  P. Sharma, M. Chawla, S. Sharma, A. Mitra, RNA 16 (2010) 942–957. doi: 10.1261/rna.1919010
23. [23]
  G. Devi, Y. Zhou, Z. Zhong, D.F.K. Toh, G. Chen, WIREs RNA 6 (2015) 111–128. doi: 10.1002/wrna.1261
24. [24]
  J.S. Paige, K.Y. Wu, S.R. Jaffrey, Science 333 (2011) 642–646. doi: 10.1126/science.1207339
25. [25]
  Y. Liu, Y. Wang, J. Lin, L. Xu, Chem. Commun. 57 (2021) 5418–5421. doi: 10.1039/D1CC01260F
26. [26]
  H. Nishimasu, F.A. Ran, P.D. Hsu, et al., Cell 156 (2014) 935–949. doi: 10.1016/j.cell.2014.02.001
27. [27]
  J. Lin, Y. Liu, P. Lai, et al., Nucleic Acids Res. 48 (2020) 11773–11784. doi: 10.1093/nar/gkaa842
28. [28]
  N. Song, S. Li, Z. Lv, et al., Chin. Chem. Lett. 34 (2023) 108134. doi: 10.1016/j.cclet.2023.108134
1. [1]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
2. [2]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
3. [3]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
4. [4]
  Ling Yang , Min Ren , Jie Wang , Liming He , Shanshan Wu , Shuai Yang , Wei Zhao , Hao Cheng , Xiaoming Zhou , Maling Gou . A non-viral gene therapy for melanoma by staphylococcal enterotoxin A. Chinese Chemical Letters, 2024, 35(5): 108822-. doi: 10.1016/j.cclet.2023.108822
5. [5]
  Jun Xiong , Ke-Ke Chen , Neng-Bin Xie , Wei Chen , Wen-Xuan Shao , Tong-Tong Ji , Si-Yu Yu , Yu-Qi Feng , Bi-Feng Yuan . Demethylase-assisted site-specific detection of N¹-methyladenosine in RNA. Chinese Chemical Letters, 2024, 35(5): 108953-. doi: 10.1016/j.cclet.2023.108953
6. [6]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
7. [7]
  Zhili Li , Qijun Wo , Dongdong Huang , Dezhong Zhou , Lei Guo , Yeqing Mao . Improving gene transfection efficiency of highly branched poly(β-amino ester)s through the in-situ conversion of inactive terminal groups. Chinese Chemical Letters, 2024, 35(8): 109737-. doi: 10.1016/j.cclet.2024.109737
8. [8]
  Wenyi Mei , Lijuan Xie , Xiaodong Zhang , Cunjian Shi , Fengzhi Wang , Qiqi Fu , Zhenjiang Zhao , Honglin Li , Yufang Xu , Zhuo Chen . Design, synthesis and biological evaluation of fluorescent derivatives of ursolic acid in living cells. Chinese Chemical Letters, 2024, 35(5): 108825-. doi: 10.1016/j.cclet.2023.108825
9. [9]
  Tianhao Li , Wenguang Tu , Zhigang Zou . In situ photocatalytically enhanced thermogalvanic cells for electricity and hydrogen production. Chinese Journal of Structural Chemistry, 2024, 43(1): 100195-100195. doi: 10.1016/j.cjsc.2023.100195
10. [10]
  Chen Lu , Zefeng Yu , Jing Cao . Advancement in porphyrin/phthalocyanine compounds-based perovskite solar cells. Chinese Journal of Structural Chemistry, 2024, 43(3): 100240-100240. doi: 10.1016/j.cjsc.2024.100240
11. [11]
  Menglu Guo , Ying-Qi Song , Junfei Cheng , Guoqiang Dong , Xun Sun , Chunquan Sheng . Hydrophobic tagging-induced degradation of NAMPT in leukemia cells. Chinese Chemical Letters, 2024, 35(9): 109392-. doi: 10.1016/j.cclet.2023.109392
12. [12]
  Chi Li , Peng Gao . Is dipole the only thing that matters for inverted perovskite solar cells?. Chinese Journal of Structural Chemistry, 2024, 43(6): 100324-100324. doi: 10.1016/j.cjsc.2024.100324
13. [13]
  Chuan-Zhi Ni , Ruo-Ming Li , Fang-Qi Zhang , Qu-Ao-Wei Li , Yuan-Yuan Zhu , Jie Zeng , Shuang-Xi Gu . A chiral fluorescent probe for molecular recognition of basic amino acids in solutions and cells. Chinese Chemical Letters, 2024, 35(10): 109862-. doi: 10.1016/j.cclet.2024.109862
14. [14]
  Shuang Li , Jiayu Sun , Guocheng Liu , Shuo Zhang , Zhong Zhang , Xiuli Wang . A new Keggin-type polyoxometallate-based bifunctional catalyst for trace detection and pH-universal photodegradation of phenol. Chinese Chemical Letters, 2024, 35(8): 109148-. doi: 10.1016/j.cclet.2023.109148
15. [15]
  Yuqing Wang , Zhemin Li , Qingjun Lu , Qizhao Li , Jiaxin Luo , Chengjie Li , Yongshu Xie . Solar cells based on doubly concerted companion dyes with the efficiencies modulated by inserting an ethynyl group at different positions. Chinese Chemical Letters, 2024, 35(5): 109093-. doi: 10.1016/j.cclet.2023.109093
16. [16]
  Yaxin Sun , Huiyu Li , Shiquan Guo , Congju Li . Metal-based cathode catalysts for electrocatalytic ORR in microbial fuel cells: A review. Chinese Chemical Letters, 2024, 35(5): 109418-. doi: 10.1016/j.cclet.2023.109418
17. [17]
  Feng Wu , Xuemin Kong , Yixuan Liu , Shuli Wang , Zhong Chen , Xu Hou . Microfluidic-based isolation of circulating tumor cells with high-efficiency and high-purity. Chinese Chemical Letters, 2024, 35(8): 109754-. doi: 10.1016/j.cclet.2024.109754
18. [18]
  Xing Tian , Di Wu , Wanheng Wei , Guifu Dai , Zhanxian Li , Benhua Wang , Mingming Yu . A lipid droplets-targetable fluorescent probe for polarity detection in cells of iron death, inflammation and fatty liver tissue. Chinese Chemical Letters, 2024, 35(6): 108912-. doi: 10.1016/j.cclet.2023.108912
19. [19]
  Xin Lu , Haoran Sun , Xiaomeng Li , Chunrui Li , Jinfeng Wang , Dandan Zhou . C14-HSL limits the mycelial morphology of pathogen Trichosporon cells but enhances their aggregation: Mechanisms and implications. Chinese Chemical Letters, 2024, 35(6): 108936-. doi: 10.1016/j.cclet.2023.108936
20. [20]
  Kangrong Yan , Ziqiu Shen , Yanchun Huang , Benfang Niu , Hongzheng Chen , Chang-Zhi Li . Curing the vulnerable heterointerface via organic-inorganic hybrid hole transporting bilayers for efficient inverted perovskite solar cells. Chinese Chemical Letters, 2024, 35(6): 109516-. doi: 10.1016/j.cclet.2024.109516

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(164)
HTML views(7)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142