Citation: Jingqi Xin, Shupeng Han, Meichen Zheng, Chenfeng Xu, Zhongxi Huang, Bin Wang, Changmin Yu, Feifei An, Yu Ren. A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance[J]. Chinese Chemical Letters, ;2024, 35(7): 109165. doi: 10.1016/j.cclet.2023.109165 shu

A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance

Jingqi Xin ^{a, b} ,
Shupeng Han ^b ,
Meichen Zheng ^b ,
Chenfeng Xu ^c ,
Zhongxi Huang ^c ,
Bin Wang ^d ,
Changmin Yu ^c ,
Feifei An ^{b, *,} ,
Yu Ren ^{d, *,}

a.
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Science, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China
b.
School of Public Health, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China
c.
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University, Nanjing 211816, China
d.
Department of Breast Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China

anfeifei@xjtu.edu.cn

renyyyyy@xjtufh.edu.cn

Received Date: 13 August 2023
Revised Date: 24 September 2023
Accepted Date: 3 October 2023
Available Online: 5 October 2023

Figures(4)

Tumor microenvironment (TME)-activatable probes have been proven to effectively increase signal-to-background ratios (SBRs) and improve the success rate of complete tumor resection. However, many fluorescence probes have to be loaded into a nanocarrier for tumor targeted delivery, which consequently encounters poor drug loading, heterogeneous composition and non-encapsulated drug aggregates occurred during nanoformulation fabrications. Herein, a nitroreductase (NTR)-activated "OFF-ON" near-infrared fluorescence nanoprobe, named NanoBodipy, was synthesized by the spontaneous self-assembling of NTR-responsive dye-polyethylene glycol (PEG) amphiphilic polymer in water. The NTR-responsive dye acted as the hydrophobic segment in the amphiphilic polymer, yielding a homogeneous composition and a high loading of 12.2 wt% (according to calculation) in the synthesized NanoBodipy. The synthesized NanoBodipy can efficiently accumulate in tumors via the enhanced permeability and retention (EPR) effect, enabling non-invasive tumor-targeted fluorescence imaging and guiding complete tumor resection. Once the synthesized NanoBodipy entered the tumor cells, they dissociated and were activated by overexpressed NTR. With the real-time fluorescence guide of NanoBodipy, complete tumor resection surgery was performed successfully.
- Nitroreductase,
- Tumor diagnosis,
- Intraoperative guidance,
- Self-assembly,
- Nanoprobe
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