Citation: Jia-Li Xie, Tian-Jin Xie, Yu-Jie Luo, Kai Mao, Cheng-Zhi Huang, Yuan-Fang Li, Shu-Jun Zhen. Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection[J]. Chinese Chemical Letters, ;2024, 35(6): 109137. doi: 10.1016/j.cclet.2023.109137 shu

Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection

a.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China
b.
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Chongqing Science and Technology Bureau, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

zsj@swu.edu.cn

Received Date: 11 May 2023
Revised Date: 4 September 2023
Accepted Date: 21 September 2023
Available Online: 25 September 2023

Figures(6)

Fluorescence Anisotropy (FA) is an effective biochemical detection method based on molecular rotations. Graphene oxide (GO) has been extensively used as an FA amplifier. However, the enhancement of FA by GO alone is limited and the strong scattering of GO will easily make the measurement of FA inaccurate. In order to address these problems, an octopus-like DNA nanostructure (ODN) was designed and coupled with GO to enhance the FA together in this work. By mimicking the multi-clawed structure of the octopus, the ODN can be adsorbed on GO tightly, which not only could improve the sensitivity because of the double FA enhancement abilities of GO and ODN, but also could improve the specificity due to the decrease of the nonspecific interaction in complex samples. Furthermore, ODN could maintain a certain distance between the fluorophore and GO to reduce the fluorescence quenching efficiency of GO, which could improve the accuracy. This method has been applied for the detection of hepatitis B virus DNA (HBV-DNA) in a range of 1–50nmol/L and the limit of detection (LOD) was 330pmol/L. In addition, the proposed method has been successfully utilized to detect HBV-DNA in human serum, indicating that this method has a great practical application prospect.
- Fluorescence anisotropy,
- Graphene oxide (GO),
- Octopus-like DNA nanostructure,
- HBV-DNA
1. [1]
  D. Zhang, M. Lu, H. Wang, J. Am. Chem. Soc. 133 (2011) 9188–9191. doi: 10.1021/ja202141y
2. [2]
  X. Xiao, S. Zhen, RSC Adv. 12 (2022) 6364–6376. doi: 10.1039/d2ra00058j
3. [3]
  K. Chullipalliyalil, K. Elkassas, M.A.P. McAuliffe, S. Vucen, A. Crean, Anal. Chem. 95 (2023) 2774–2782. doi: 10.1021/acs.analchem.2c03912
4. [4]
  D. Zhang, H. Wang, Anal. Chem. 91 (2019) 14538–14544. doi: 10.1021/acs.analchem.9b03556
5. [5]
  W. Huang, C. Guo, J. Zhai, X. Xie, Anal. Chem. 94 (2022) 9793–9800. doi: 10.1021/acs.analchem.2c01532
6. [6]
  Q. Huang, B. Chen, J. Shen, et al., J. Am. Chem. Soc. 143 (2021) 10735–10742. doi: 10.1021/jacs.1c04942
7. [7]
  J. Chen, J. Liu, X. Chen, H. Qiu, Chin. Chem. Lett. 30 (2019) 1575–1580. doi: 10.1016/j.cclet.2019.06.005
8. [8]
  S. Jain, A. Heiser, A.R. Venter, Analyst 136 (2011) 1298–1301. doi: 10.1039/c0an00728e
9. [9]
  T.L. Mann, U.J. Krull, Analyst 128 (2003) 313–317. doi: 10.1039/b300873h
10. [10]
  Y.L. Fan, Z.Y. Liu, Y.M. Zeng, et al., Talanta 223 (2021) 121721. doi: 10.1016/j.talanta.2020.121721
11. [11]
  B. Yang, X.B. Zhang, L.P. Kang, et al., Anal. Chem. 85 (2013) 11518–11523. doi: 10.1021/ac402781g
12. [12]
  C. Zeng, J. Gao, Y. Lou, L. Cui, Talanta 218 (2020) 121179. doi: 10.1016/j.talanta.2020.121179
13. [13]
  M. Zou, Y. Chen, X. Xu, et al., Biosens. Bioelectron. 32 (2012) 148–154. doi: 10.1016/j.bios.2011.11.052
14. [14]
  A. Pepanian, P. Sommerfeld, R. Kasprzyk, et al., Anal. Chem. 94 (2022) 14410–14418. doi: 10.1021/acs.analchem.2c03176
15. [15]
  B.C. Yin, P. Zuo, H. Huo, X. Zhong, B.C. Ye, Anal. Biochem. 401 (2010) 47–52. doi: 10.1016/j.ab.2010.02.014
16. [16]
  Y. Yu, Y. Liu, S.J. Zhen, C.Z. Huang, Chem. Commun. 49 (2013) 1942–1944. doi: 10.1039/c3cc38129c
17. [17]
  B.C. Ye, B.C. Yin, Angew. Chem. Int. Ed. 47 (2008) 8386–8389. doi: 10.1002/anie.200803069
18. [18]
  J. Zhang, J. Tian, Y. He, Y. Zhao, S. Zhao, Chem. Commun. 50 (2014) 2049–2051. doi: 10.1039/C3CC49424A
19. [19]
  X. Xiao, J. Tao, H.Z. Zhang, C.Z. Huang, S.J. Zhen, Biosens. Bioelectron. 85 (2016) 822–827. doi: 10.1016/j.bios.2016.05.091
20. [20]
  Y.X. Liu, X. Xiao, C.H. Li, et al., Talanta 211 (2020) 120730. doi: 10.1016/j.talanta.2020.120730
21. [21]
  Q. Zhao, Q. Lv, H. Wang, Anal. Chem. 86 (2014) 1238–1245. doi: 10.1021/ac4035532
22. [22]
  Y. Li, H. Yu, Q. Zhao, RSC Adv. 12 (2022) 7464–7468. doi: 10.1039/d2ra00843b
23. [23]
  C. Hoffmann, M. Jourdain, A. Grandjean, A. Titz, G. Jung, Anal. Chem. 94 (2022) 6112–6119. doi: 10.1021/acs.analchem.1c04654
24. [24]
  X. Xiao, Y.F. Li, C.Z. Huang, S.J. Zhen, Chem. Commun. 51 (2015) 16080–16083. doi: 10.1039/C5CC05902J
25. [25]
  S.J. Zhen, X. Xiao, C.H. Li, C.Z. Huang, Anal. Chem. 89 (2017) 8766–8771. doi: 10.1021/acs.analchem.7b00955
26. [26]
  B. Billet, B. Chovelon, E. Fiore, et al., Angew. Chem. Int. Ed. 60 (2021) 12346–12350. doi: 10.1002/anie.202102254
27. [27]
  L. Gao, L. Liu, Y. Tian, et al., Anal. Chem. 93 (2021) 7045–7053. doi: 10.1021/acs.analchem.1c00363
28. [28]
  E. Sijbesma, B.A. Somsen, G.P. Miley, et al., ACS Chem. Biol. 15 (2020) 3143–3148. doi: 10.1021/acschembio.0c00646
29. [29]
  Q.H. Wan, X.C. Le, Anal. Chem. 72 (2000) 5583–5589. doi: 10.1021/ac000318+
30. [30]
  Y. Li, L. Sun, Q. Zhao, Talanta 174 (2017) 7–13. doi: 10.1016/j.talanta.2017.05.077
31. [31]
  Y. Li, Q. Zhao, Anal. Chem. 91 (2019) 7379–7384. doi: 10.1021/acs.analchem.9b01253
32. [32]
  Z. Zhu, T. Schmidt, M. Mahrous, et al., Anal. Chim. Acta 707 (2011) 191–196. doi: 10.1016/j.aca.2011.09.022
33. [33]
  S. Perrier, P. Bouilloud, G. De Oliveira Coelho, M. Henry, E. Peyrin, Biosens. Bioelectron. 82 (2016) 155–161. doi: 10.1016/j.bios.2016.04.010
34. [34]
  Y. Huang, S. Zhao, Z.F. Chen, Y.C. Liu, H. Liang, Chem. Commun. 47 (2011) 4763–4765. doi: 10.1039/c1cc10325c
35. [35]
  N. Mohanty, V. Berry, Nano Lett. 8 (2008) 4469–4476. doi: 10.1021/nl802412n
36. [36]
  F. Kim, L.J. Cote, J. Huang, Adv. Mater. 22 (2010) 1954–1958. doi: 10.1002/adma.200903932
37. [37]
  Y. Hu, F. Li, D. Han, et al., Anal. Chim. Acta. 753 (2012) 82–89. doi: 10.1016/j.aca.2012.09.038
38. [38]
  Y. Piao, F. Liu, T.S. Seo, Chem. Commun. 47 (2011) 12149–12151. doi: 10.1039/c1cc15043j
39. [39]
  T. Miyahata, Y. Kitamura, A. Futamura, et al., Chem. Commun. 49 (2013) 10139–10141. doi: 10.1039/c3cc45531a
40. [40]
  L. Gao, C. Lian, Y. Zhou, et al., Biosens. Bioelectron. 60 (2014) 22–29. doi: 10.1016/j.bios.2014.03.039
41. [41]
  Q. Tang, Q. Zhang, Y. Jiang, et al., ACS Appl. Mater. Inter. 6 (2014) 13470–13477. doi: 10.1021/am502311b
42. [42]
  A.E. Prigodich, O.S. Lee, W.L. Daniel, et al., J. Am. Chem. Soc. 132 (2010) 10638–10641. doi: 10.1021/ja104859j
43. [43]
  Y. Chen, D. Tyagi, M. Lyu, et al., Anal. Chem. 91 (2019) 4017–4022. doi: 10.1021/acs.analchem.8b05432
44. [44]
  G. Kang, C.A.H. Allard, W.A. Valencia-Montoya, et al., Nature 616 (2023) 378–383. doi: 10.1038/s41586-023-05808-z
45. [45]
  J.H. Choi, M. Shin, L. Yang, et al., ACS Nano 15 (2021) 13475–13485. doi: 10.1021/acsnano.1c03975
46. [46]
  Y. Liang, W.L. Ang, R.R.X. Lim, A. Bonanni, Chem. Commun. 58 (2022) 2662–2665. doi: 10.1039/d1cc06657a
47. [47]
  K. Ma, W. Xie, W. Liu, et al., Adv. Funct. Mater. 31 (2021) 2102645. doi: 10.1002/adfm.202102645
48. [48]
  J. Vilcapoma, A. Patel, A.R. Chandrasekara, K. Halvorsen, Chem. Commun. 59 (2023) 5083–5085. doi: 10.1039/d3cc01123b
49. [49]
  Y.Z. Guo, J.L. Liu, Y.F. Chen, et al., Anal. Chem. 94 (2022) 7601–7608. doi: 10.1021/acs.analchem.2c00763
50. [50]
  S. Pfister, J. Rabl, T. Wiegand, et al., Nat. Commun. 14 (2023) 1574. doi: 10.1038/s41467-023-37068-w
51. [51]
  J. Su, W. Liu, S. Chen, et al., ACS Sens. 5 (2020) 3979–3987. doi: 10.1021/acssensors.0c01745
1. [1]
  Chang Liu , Tao Wu , Lijiao Deng , Xuzi Li , Xin Fu , Shuzhen Liao , Wenjie Ma , Guoqiang Zou , Hai Yang . Programmed DNA walkers for biosensors. Chinese Chemical Letters, 2024, 35(9): 109307-. doi: 10.1016/j.cclet.2023.109307
2. [2]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
3. [3]
  Xiaohong Wen , Mei Yang , Lie Li , Mingmin Huang , Wei Cui , Suping Li , Haiyan Chen , Chen Li , Qiuping Guo . Enzymatically controlled DNA tetrahedron nanoprobes for specific imaging of ATP in tumor. Chinese Chemical Letters, 2024, 35(8): 109291-. doi: 10.1016/j.cclet.2023.109291
4. [4]
  Jingwen Zhao , Jianpu Tang , Zhen Cui , Limin Liu , Dayong Yang , Chi Yao . A DNA micro-complex containing polyaptamer for exosome separation and wound healing. Chinese Chemical Letters, 2024, 35(9): 109303-. doi: 10.1016/j.cclet.2023.109303
5. [5]
  Ying Chen , Li Li , Junyao Zhang , Tongrui Sun , Xuan Zhang , Shiqi Zhang , Jia Huang , Yidong Zou . Tailored ionically conductive graphene oxide-encased metal ions for ultrasensitive cadaverine sensor. Chinese Chemical Letters, 2024, 35(8): 109102-. doi: 10.1016/j.cclet.2023.109102
6. [6]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
7. [7]
  Zhe-Han Yang , Jie Yin , Lei Xin , Yuanfang Li , Yijie Huang , Ruo Yuan , Ying Zhuo . Research advancement of DNA-based intelligent hydrogels: Manufacture, characteristics, application of disease diagnosis and treatment. Chinese Chemical Letters, 2024, 35(10): 109558-. doi: 10.1016/j.cclet.2024.109558
8. [8]
  Tao Long , Peng Chen , Bin Feng , Caili Yang , Kairong Wang , Yulei Wang , Can Chen , Yaping Wang , Ruotong Li , Meng Wu , Minhuan Lan , Wei Kong Pang , Jian-Fang Wu , Yuan-Li Ding . Reinforced concrete-like Na_3.5V_1.5Mn_0.5(PO₄)₃@graphene hybrids with hierarchical porosity as durable and high-rate sodium-ion battery cathode. Chinese Chemical Letters, 2024, 35(4): 109267-. doi: 10.1016/j.cclet.2023.109267
9. [9]
  Deshuai Zhen , Chunlin Liu , Qiuhui Deng , Shaoqi Zhang , Ningman Yuan , Le Li , Yu Liu . A review of covalent organic frameworks for metal ion fluorescence sensing. Chinese Chemical Letters, 2024, 35(8): 109249-. doi: 10.1016/j.cclet.2023.109249
10. [10]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
11. [11]
  Ying Xu , Chengying Shen , Hailong Yuan , Wei Wu . Mapping multiple phases in curcumin binary solid dispersions by fluorescence contrasting. Chinese Chemical Letters, 2024, 35(9): 109324-. doi: 10.1016/j.cclet.2023.109324
12. [12]
  Yuxin Li , Chengbin Liu , Qiuju Li , Shun Mao . Fluorescence analysis of antibiotics and antibiotic-resistance genes in the environment: A mini review. Chinese Chemical Letters, 2024, 35(10): 109541-. doi: 10.1016/j.cclet.2024.109541
13. [13]
  Haodong Wang , Xiaoxu Lai , Chi Chen , Pei Shi , Houzhao Wan , Hao Wang , Xingguang Chen , Dan Sun . Novel 2D bifunctional layered rare-earth hydroxides@GO catalyst as a functional interlayer for improved liquid-solid conversion of polysulfides in lithium-sulfur batteries. Chinese Chemical Letters, 2024, 35(5): 108473-. doi: 10.1016/j.cclet.2023.108473
14. [14]
  Tian Cao , Xuyin Ding , Qiwen Peng , Min Zhang , Guoyue Shi . Intelligent laser-induced graphene sensor for multiplex probing catechol isomers. Chinese Chemical Letters, 2024, 35(7): 109238-. doi: 10.1016/j.cclet.2023.109238
15. [15]
  Rui Liu , Jinbo Pang , Weijia Zhou . Monolayer water shepherding supertight MXene/graphene composite films. Chinese Journal of Structural Chemistry, 2024, 43(10): 100329-100329. doi: 10.1016/j.cjsc.2024.100329
16. [16]
  Shuwen SUN , Gaofeng WANG . Two cadmium coordination polymers constructed by varying Ⅴ-shaped co-ligands: Syntheses, structures, and fluorescence properties. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 613-620. doi: 10.11862/CJIC.20230368
17. [17]
  Ruikui YAN , Xiaoli CHEN , Miao CAI , Jing REN , Huali CUI , Hua YANG , Jijiang WANG . Design, synthesis, and fluorescence sensing performance of highly sensitive and multi-response lanthanide metal-organic frameworks. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 834-848. doi: 10.11862/CJIC.20230301
18. [18]
  Zhiqiang Liu , Qiang Gao , Wei Shen , Meifeng Xu , Yunxin Li , Weilin Hou , Hai-Wei Shi , Yaozuo Yuan , Erwin Adams , Hian Kee Lee , Sheng Tang . Removal and fluorescence detection of antibiotics from wastewater by layered double oxides/metal-organic frameworks with different topological configurations. Chinese Chemical Letters, 2024, 35(8): 109338-. doi: 10.1016/j.cclet.2023.109338
19. [19]
  Zhichao Zhou , Fuqian Chen , Xiaotong Xia , Dong Ye , Rong Zhou , Lei Li , Tao Deng , Zhenhua Ding , Fang Liu . Developing a fluorescence substrate for HRP-based diagnostic assays with superiorities over the commercial ADHP. Chinese Chemical Letters, 2024, 35(6): 108970-. doi: 10.1016/j.cclet.2023.108970
20. [20]
  Zixi Zou , Jingyuan Wang , Yian Sun , Qian Wang , Da-Hui Qu . Controlling molecular assembly on time scale: Time-dependent multicolor fluorescence for information encryption. Chinese Chemical Letters, 2024, 35(7): 108972-. doi: 10.1016/j.cclet.2023.108972

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(139)
HTML views(4)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142