Citation: Huaran Zhang, Yuting Huang, Yingjie Tang, Dekun Kong, Yi Zou. Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing- and type Ⅲ- polyketide pathway[J]. Chinese Chemical Letters, ;2024, 35(7): 108968. doi: 10.1016/j.cclet.2023.108968 shu

Genome mining of multi-substituted alkylresorcinols from a hybrid highly reducing- and type Ⅲ- polyketide pathway

College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

kongdekun@swu.edu.cn

zouyi31@swu.edu.cn

Received Date: 16 July 2023
Revised Date: 19 August 2023
Accepted Date: 24 August 2023
Available Online: 3 September 2023

Figures(3)

Fungal alkylresorcinols are a class of polyketides, which are commonly synthesized by the hybridization of highly reducing polyketide synthase (hrPKS) with non-reducing polyketide synthase (nrPKS). In this study, we identified and demonstrated a new assembly model for synthesizing alkylresorcinol (scirpilin A, 1), which was accomplished by collaboration of a hrPKS (FscA) and a type Ⅲ PKS (FscB). Furthermore, three post-tailoring enzymes (FscC, FscD, and FscE) act iteratively on 1 skeleton, including successive 14e⁻ oxidation of inert carbons, di-halogenation, and di-methylation, to form highly oxidized and multi-substituted alkylresorcinols. Our work presents an unusual synthesis manner of alkylresorcinols, sheds light on the collaborative mechanism between hrPKS and type Ⅲ PKS and provides three valuable enzymatic catalysts for the tailoring of alkylresorcinol family natural products in future.
- Genome mining,
- Alkylresorcinol,
- hrPKS,
- Type Ⅲ PKS,
- Iterative catalysis
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