Citation: Hao Cai, Xiaoyan Wu, Lei Jiang, Feng Yu, Yuxiang Yang, Yan Li, Xian Zhang, Jian Liu, Zijian Li, Hong Bi. Lysosome-targeted carbon dots with a light-controlled nitric oxide releasing property for enhanced photodynamic therapy[J]. Chinese Chemical Letters, ;2024, 35(4): 108946. doi: 10.1016/j.cclet.2023.108946 shu

Lysosome-targeted carbon dots with a light-controlled nitric oxide releasing property for enhanced photodynamic therapy

Hao Cai ^{a, 1} ,
Xiaoyan Wu ^{a, 1} ,
Lei Jiang ^b ,
Feng Yu ^b ,
Yuxiang Yang ^b ,
Yan Li ^a ,
Xian Zhang ^c ,
Jian Liu ^c ,
Zijian Li ^{a, *,} ,
Hong Bi ^{a, *,}

a.
School of Materials Science and Engineering, Anhui University, Hefei 230601, China
b.
School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China
c.
School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China

22018@ahu.edu.cn

bihong@ahu.edu.cn

Received Date: 22 May 2023
Revised Date: 17 August 2023
Accepted Date: 18 August 2023
Available Online: 19 August 2023

Figures(6)

The reactive oxygen species (ROS) generation efficiency is always limited by the extreme tumor microenvironment (TME), leading to unsatisfactory antitumor effects in photodynamic therapy (PDT). As a promising gas therapy molecule, nitric oxide (NO) is independent of oxygen and could even synergize ROS to enhance the therapeutic effect. However, the short half-life, instability, and uncontrollable release of exogenous NO limited the application of tumor synergistic therapy. Herein, we reported a novel kind of red-emissive carbon dots (CDs) that was capable of lysosome-targeted and light-controlled NO delivery. The CDs were synthesized by using metformin and methylene blue (MB) via a hydrothermal method. The obtained metformin-MB CDs (MMCDs) exhibited a higher ¹O₂ quantum yield and NO generation efficiency under light emitting diode (LED) light irradiation. Noteworthily, the ¹O₂ could further in situ oxidize NO into peroxynitrite anions (ONOO⁻), which own the higher cytotoxicity against cancer cells. Cell experiments indicate that MMCDs could destruct lysosome membrane integrity and kill almost 80% of HepG2 cells under light irradiation while very low cytotoxicity in the dark. Moreover, MMCDs significantly decreased tumor volume and weight after phototherapy in hepatoma HepG2-bearing mice. Our study provides a new strategy for light-controlled NO generation as well as precise lysosome-targeting for enhancement of PDT efficiency.
- Carbon dots,
- Nitric oxide,
- Lysosome-targeting,
- Photodynamic therapy,
- Cancer
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沈阳化工大学材料科学与工程学院沈阳 110142