Citation: Jing Chen, Peisi Xie, Pengfei Wu, Yu He, Zian Lin, Zongwei Cai. MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids[J]. Chinese Chemical Letters, ;2024, 35(4): 108895. doi: 10.1016/j.cclet.2023.108895 shu

MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids

Jing Chen ^{a, 1} ,
Peisi Xie ^{a, 1} ,
Pengfei Wu ^{b, c} ,
Yu He ^a ,
Zian Lin ^a ,
Zongwei Cai ^{a, b, *,}

a.
Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou 350116, China
b.
Department of Chemistry, State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong, China
c.
College of Forestry, Nanjing Forestry University, Nanjing 210018, China

zwcai@hkbu.edu.hk

Received Date: 29 March 2023
Revised Date: 23 July 2023
Accepted Date: 4 August 2023
Available Online: 5 August 2023

Figures(4)

Cancer cell spheroids (CCS) are a valuable three-dimensional cell model in cancer studies because they could replicate numerous characteristics of solid tumors. Increasing researches have used matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) to investigate the spatial distribution of endogenous compounds (e.g., lipids) in CCS. However, only limited lipid species can be detected owing to a low ion yield by using MALDI. Besides, it is still challenging to fully characterize the structural diversity of lipids due to the existence of isomeric/isobaric species. Here, we carried out the initial application of MALDI coupled with laser-postionization (MALDI-2) and trapped ion mobility spectrometry (TIMS) imaging in HCT116 colon CCS to address these challenges. We demonstrated that MALDI-2 is capable of detecting more number and classes of lipids in HCT116 colon CCS with higher signal intensities than MALDI. TIMS could successfully separate numerous isobaric/isomeric species of lipids in CCS. Interestingly, we found that some isomeric/isobaric species have totally different spatial distributions in colon CCS. Further MS/MS imaging analysis was employed to determine the compositions of fatty acid chains for isomeric species by examining disparities in signal intensities and spatial distributions of product ions. This work stresses the robust ability of TIMS and MALDI-2 imaging in analyzing endogenous lipids in CCS, which could potentially become powerful tools for future cancer studies.
1. [1]
  S. Breslin, L. O'Driscoll, Drug Discov. Today 18 (2013) 240–249. doi: 10.1016/j.drudis.2012.10.003
2. [2]
  M. Xie, C. Huang, Y. Liang, et al., Chin. Chem. Lett. 33 (2022) 293–297. doi: 10.1016/j.cclet.2021.06.049
3. [3]
  Y. Wang, A.B. Hummon, J. Biol. Chem. 297 (2021) 101139. doi: 10.1016/j.jbc.2021.101139
4. [4]
  J. Zhang, Q. Du, X. Song, et al., Theranostics 10 (2020) 2621–2630. doi: 10.7150/thno.41763
5. [5]
  T. Sonoda, H. Kobayashi, T. Kaku, T. Hirakawa, H. Nakano, Cancer Lett. 196 (2003) 229–237. doi: 10.1016/S0304-3835(03)00216-7
6. [6]
  P. Xie, J. Zhang, P. Wu, et al., Chin. Chem. Lett. 34 (2023) 107349. doi: 10.1016/j.cclet.2022.03.072
7. [7]
  P. Xie, H. Zhang, P. Wu, Y. Chen, Z. Cai, Anal. Chem. 94 (2022) 13667–13675. doi: 10.1021/acs.analchem.2c00768
8. [8]
  C. Zhao, P. Xie, T. Yang, et al., Chin. Chem. Lett. 29 (2018) 1281–1283. doi: 10.1016/j.cclet.2018.01.034
9. [9]
  G.J. LaBonia, K.R. Ludwig, C.B. Mousseau, A.B. Hummon, Anal. Chem. 90 (2018) 1423–1430. doi: 10.1021/acs.analchem.7b04969
10. [10]
  W.H. Müller, A. Verdin, E. De Pauw, C. Malherbe, G. Eppe, Mass Spectrom. Rev. 41 (2022) 373–420. doi: 10.1002/mas.21670
11. [11]
  T. Shirota, M. Tsuge, Y. Hikosaka, K. Soejima, K.J. Hoshina, Phys. Chem. A 121 (2017) 31–39. doi: 10.1021/acs.jpca.6b09591
12. [12]
  S.R. Ellis, J. Soltwisch, M.R.L. Paine, K. Dreisewerd, R.M.A. Heeren, Chem. Commun. 53 (2017) 7246–7249. doi: 10.1039/C7CC02325A
13. [13]
  J. Liu, Q. Zang, X. Li, et al., Chin. Chem. Lett. 34 (2023) 108322. doi: 10.1016/j.cclet.2023.108322
14. [14]
  C. Harkin, K.W. Smith, F.L. Cruickshank, et al., Mass Spectrom. Rev. 41 (2022) 662–694. doi: 10.1002/mas.21680
15. [15]
  J. Li, L. Qin, D. Jiang, et al., Sci. Sin. Chim. 52 (2022) 1041–1073. doi: 10.1360/SSC-2022-0009
16. [16]
  B. Heijs, A. Potthoff, J. Soltwisch, K. Dreisewerd, Anal. Chem. 92 (2020) 13904–13911. doi: 10.1021/acs.analchem.0c02732
17. [17]
  R. Zecchi, P. Franceschi, L. Tigli, et al., Anal. Bioanal. Chem. 413 (2021) 4363–4371. doi: 10.1007/s00216-021-03393-6
18. [18]
  J. Soltwisch, B. Heijs, A. Koch, et al., Anal. Chem. 92 (2020) 8697–8703. doi: 10.1021/acs.analchem.0c01747
19. [19]
  L. Hanley, R. Wickramasinghe, Y.P. Yung, Annu. Rev. Anal. Chem. 12 (2019) 225–245. doi: 10.1146/annurev-anchem-061318-115447
20. [20]
  C. Bookmeyer, U. Röhling, K. Dreisewerd, J. Soltwisch, Angew. Chem. Int. Ed. 134 (2022) e202202165. doi: 10.1002/ange.202202165
21. [21]
  J. Soltwisch, H. Kettling, S. Vens-Cappell, et al., Science 348 (2015) 211–215. doi: 10.1126/science.aaa1051
22. [22]
  J.C. McMillen, J.A. Fincher, D.R. Klein, J.M. Spraggins, R.M. Caprioli, J. Mass Spectrom. 55 (2020) e4663. doi: 10.1002/jms.4663
23. [23]
  M. Liang, D. Liu, Y. Nie, Y. Liu, X. Qiao, Chin. Chem. Lett. 33 (2022) 3123–3126. doi: 10.1016/j.cclet.2021.10.008
24. [24]
  L. Xiang, L. Zhu, Y. Huang, Z. Cai, Small Methods 4 (2020) 2000160. doi: 10.1002/smtd.202000160
25. [25]
  L. Zhang, B. Zhu, Y. Zeng, et al., Cancer Lett. 470 (2020) 75–83. doi: 10.1016/j.canlet.2019.08.014
26. [26]
  C. Sun, C. Ma, L. Li, et al., Chin. Chem. Lett. 33 (2022) 2073–2076. doi: 10.1016/j.cclet.2021.08.034
27. [27]
  S. Tang, H. Cheng, X. Yan, Angew. Chem. Int. Ed. 59 (2020) 209–214. doi: 10.1002/anie.201911070
28. [28]
  P. Xie, C. Zhao, W. Huang, et al., Sci. Total Environ. 657 (2019) 1389–1397. doi: 10.1016/j.scitotenv.2018.12.007
29. [29]
  S. Azab, R. Ly, P. Britz-McKibbin, Anal. Chem. 91 (2018) 2329–2336. doi: 10.1021/acs.analchem.8b05054
30. [30]
  K.E. Burnum-Johnson, X. Zheng, J.N. Dodds, et al., Trends Anal. Chem. 116 (2019) 292–299. doi: 10.1016/j.trac.2019.04.022
31. [31]
  G. Paglia, A.J. Smith, G. Astarita, Mass Spectrom. Rev. 41 (2022) 722–765. doi: 10.1002/mas.21686
32. [32]
  X. Garcia, M.M. Sabate, J. Aubets, J.M. Jansat, S. Sentellas, Separations 8 (2021) 33. doi: 10.3390/separations8030033
33. [33]
  M.E. Ridgeway, M. Lubeck, J. Jordens, M. Mann, M.A. Park, Int. J. Mass Spectrom. 425 (2018) 22–35. doi: 10.1016/j.ijms.2018.01.006
34. [34]
  K. Skeene, K. Khatri, Z. Soloviev, C. Lapthorn, Biochim. Biophys. Acta Proteins Proteom. 1869 (2021) 140697. doi: 10.1016/j.bbapap.2021.140697
35. [35]
  P.O. Helmer, I.D. Nordhorn, A. Korf, et al., Anal. Chem. 93 (2021) 2135–2143. doi: 10.1021/acs.analchem.0c03942
36. [36]
  M.S. Boskamp, J. Soltwisch, Anal. Chem. 92 (2020) 5222–5230. doi: 10.1021/acs.analchem.9b05761
37. [37]
  X. Mao, J. He, T. Li, et al., Sci. Rep. 6 (2016) 21043. doi: 10.1038/srep21043
38. [38]
  M. Zhang, J. He, T. Li, et al., Fron. Oncol. 9 (2019) 804. doi: 10.3389/fonc.2019.00804
39. [39]
  S.I. Lee, Y.Y. Choi, S.G. Kang, et al., Polymers 14 (2022) 3752. doi: 10.3390/polym14183752
40. [40]
  R. Barrera-Rodríguez, J.M. Fuentes, Cancer Cell Int. 15 (2015) 1–12. doi: 10.1186/s12935-015-0156-6
41. [41]
  Q. Zang, C. Sun, X. Chu, et al., Anal. Chim. Acta 1155 (2021) 338342. doi: 10.1016/j.aca.2021.338342
1. [1]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
2. [2]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
3. [3]
  Hualei Xu , Manman Han , Haiqiang Liu , Liang Qin , Lulu Chen , Hao Hu , Ran Wu , Chenyu Yang , Hua Guo , Jinrong Li , Jinxiang Fu , Qichen Hao , Yijun Zhou , Jinchao Feng , Xiaodong Wang . 4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI. Chinese Chemical Letters, 2024, 35(6): 109095-. doi: 10.1016/j.cclet.2023.109095
4. [4]
  Boran Cheng , Lei Cao , Chen Li , Fang-Yi Huo , Qian-Fang Meng , Ganglin Tong , Xuan Wu , Lin-Lin Bu , Lang Rao , Shubin Wang . Fluorine-doped carbon quantum dots with deep-red emission for hypochlorite determination and cancer cell imaging. Chinese Chemical Letters, 2024, 35(6): 108969-. doi: 10.1016/j.cclet.2023.108969
5. [5]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
6. [6]
  Dan Ouyang , Huan Huang , Yanting He , Jiajing Chen , Jiali Lin , Zhuling Chen , Zongwei Cai , Zian Lin . Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides. Chinese Chemical Letters, 2024, 35(5): 108885-. doi: 10.1016/j.cclet.2023.108885
7. [7]
  Yang Liu , Yan Liu , Kaiyin Yang , Zhiruo Zhang , Wenbo Zhang , Bingyou Yang , Hua Li , Lixia Chen . A selective HK2 degrader suppresses SW480 cancer cell growth by degrading HK2. Chinese Chemical Letters, 2024, 35(8): 109264-. doi: 10.1016/j.cclet.2023.109264
8. [8]
  Zhixue Liu , Haiqi Chen , Lijuan Guo , Xinyao Sun , Zhi-Yuan Zhang , Junyi Chen , Ming Dong , Chunju Li . Luminescent terphen[3]arene sulfate-activated FRET assemblies for cell imaging. Chinese Chemical Letters, 2024, 35(9): 109666-. doi: 10.1016/j.cclet.2024.109666
9. [9]
  Tian Cao , Xuyin Ding , Qiwen Peng , Min Zhang , Guoyue Shi . Intelligent laser-induced graphene sensor for multiplex probing catechol isomers. Chinese Chemical Letters, 2024, 35(7): 109238-. doi: 10.1016/j.cclet.2023.109238
10. [10]
  Zhi Wang , Lingpeng Yan , Yelin Hao , Jingxia Zheng , Yongzhen Yang , Xuguang Liu . Highly efficient and photothermally stable CDs@ZIF-8 for laser illumination. Chinese Chemical Letters, 2024, 35(10): 109430-. doi: 10.1016/j.cclet.2023.109430
11. [11]
  Ling-Ling Wu , Xiangchuan Meng , Qingyang Zhang , Xiaowan Han , Feiya Yang , Qinghua Wang , Hai-Yu Hu , Nianzeng Xing . Heavy-atom engineered hypoxia-responsive probes for precisive photoacoustic imaging and cancer therapy. Chinese Chemical Letters, 2024, 35(4): 108663-. doi: 10.1016/j.cclet.2023.108663
12. [12]
  Jianqiu Li , Yi Zhang , Songen Liu , Jie Niu , Rong Zhang , Yong Chen , Yu Liu . Cucurbit[8]uril-based non-covalent heterodimer realized NIR cell imaging through topological transformation from nanowire to nanorod. Chinese Chemical Letters, 2024, 35(10): 109645-. doi: 10.1016/j.cclet.2024.109645
13. [13]
  Xuejian Xing , Pan Zhu , E Pang , Shaojing Zhao , Yu Tang , Zheyu Hu , Quchang Ouyang , Minhuan Lan . D-A-D-structured boron-dipyrromethene with aggregation-induced enhanced phototherapeutic efficiency for near-infrared fluorescent and photoacoustic imaging-guided synergistic photodynamic and photothermal cancer therapy. Chinese Chemical Letters, 2024, 35(10): 109452-. doi: 10.1016/j.cclet.2023.109452
14. [14]
  Wantong Zhang , Zixing Xu , Guofei Dai , Zhijian Li , Chunhui Deng . Removal of Microcystin-LR in lake water sample by hydrophilic mesoporous silica composites under high-throughput MALDI-TOF MS detection platform. Chinese Chemical Letters, 2024, 35(5): 109135-. doi: 10.1016/j.cclet.2023.109135
15. [15]
  Ying Hou , Zhen Liu , Xiaoyan Liu , Zhiwei Sun , Zenan Wang , Hong Liu , Weijia Zhou . Laser constructed vacancy-rich TiO_2-x/Ti microfiber via enhanced interfacial charge transfer for operando extraction-SERS sensing. Chinese Chemical Letters, 2024, 35(9): 109634-. doi: 10.1016/j.cclet.2024.109634
16. [16]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
17. [17]
  Kexin Yuan , Yulei Liu , Haoran Feng , Yi Liu , Jun Cheng , Beiyang Luo , Qinglian Wu , Xinyu Zhang , Ying Wang , Xian Bao , Wanqian Guo , Jun Ma . Unlocking the potential of thin-film composite reverse osmosis membrane performance: Insights from mass transfer modeling. Chinese Chemical Letters, 2024, 35(5): 109022-. doi: 10.1016/j.cclet.2023.109022
18. [18]
  Haoyang Wang , Ronghao Zhang , Yanlun Ren , Li Zhang . A convenient method for measuring gas-liquid volumetric mass transfer coefficient in micro reactors. Chinese Chemical Letters, 2024, 35(4): 108833-. doi: 10.1016/j.cclet.2023.108833
19. [19]
  Junjie Wang , Yan Wang , Zhengdong Li , Changqiang Xie , Musammir Khan , Xingzhou Peng , Fabiao Yu . Triphenylamine-AIEgens photoactive materials for cancer theranostics. Chinese Chemical Letters, 2024, 35(6): 108934-. doi: 10.1016/j.cclet.2023.108934
20. [20]
  Xingwen Cheng , Haoran Ren , Jiangshan Luo . Boosting the self-trapped exciton emission in vacancy-ordered double perovskites via supramolecular assembly. Chinese Journal of Structural Chemistry, 2024, 43(6): 100306-100306. doi: 10.1016/j.cjsc.2024.100306

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(99)
HTML views(0)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142