Citation: Jingyi Zhan, Feng Shi, Juan Li, Hao Zeng, Ming Chen, Xiaoya Hu, Zhanjun Yang. DNAzyme@CuSNPs dual mimic enzyme probe-amplified chemiluminescent imaging array immunosensor for multiple chicken cytokines detection[J]. Chinese Chemical Letters, ;2023, 34(12): 108791. doi: 10.1016/j.cclet.2023.108791 shu

DNAzyme@CuSNPs dual mimic enzyme probe-amplified chemiluminescent imaging array immunosensor for multiple chicken cytokines detection

Jingyi Zhan ^{a, 1} ,
Feng Shi ^{a, 1} ,
Juan Li ^a ,
Hao Zeng ^{b, c, *,} ,
Ming Chen ^{a, *,} ,
Xiaoya Hu ^a ,
Zhanjun Yang ^{a, *,}

a.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou 225002, China
b.
National Engineering Research Center of Immunological, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy and Laboratory Medicine, Third Military Medical University, Chongqing 400037, China
c.
State Key Laboratory of Trauma, Burn and Combined Injury, Third Military Medical University, Chongqing 400037, China

zeng1109@163.com

chenming@yzu.edu.cn

zjyang@yzu.edu.cn

Received Date: 13 September 2022
Revised Date: 5 July 2023
Accepted Date: 6 July 2023
Available Online: 8 July 2023

Figures(5)

The detection of cytokines plays an important role in clinical diagnosis and immune mechanism research of chicken diseases. In this work, a novel and ultrasensitive chemiluminescent (CL) imaging array immunosensor was proposed to detect multiple chicken cytokines based on DNAzyme@CuS nanoparticles (DNAzyme@CuSNPs) dual mimic enzyme signal amplification strategy. DNAzyme@CuSNPs owns excellent peroxidase property, which was modified with second antibody (Ab₂) to prepare DNAzyme@CuSNPs detection probe, and demonstrated high catalysis CL imaging signal due to synergistic catalysis. Chicken interleukin-4 (ChIL-4) and chicken interferon-γ (ChIFN-γ) were used as model analysis samples, the DNAzyme@CuSNPs-based CL imaging immunosensor achieved simultaneous and high-throughput detection of ChIL-4 and ChIFN-γ with wide linear range of 10⁻³–10² ng/mL, and the detection limits are 0.41 pg/mL and 0.36 pg/mL, respectively. The multiplex chicken cytokines CL imaging array immunosensor shows a high sensitivity, wide linear range, excellent specificity and acceptable stability. This research opens dual mimic enzyme signal-amplified strategy to develop sensitive CL imaging immunoassay for chicken diseases detection application.
- DNAzyme@CuSNPs,
- Dual mimic enzyme,
- Signal amplification,
- Chemiluminescence,
- Array sensor,
- Chicken cytokines
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