Citation: Tiantian Li, Ruochen Jin, Bin Wu, Dongming Lan, Yunjian Ma, Yonghua Wang. A novel insight of enhancing the hydrogen peroxide tolerance of unspecific peroxygenase from Daldinia caldariorum based on structure[J]. Chinese Chemical Letters, ;2024, 35(4): 108701. doi: 10.1016/j.cclet.2023.108701 shu

A novel insight of enhancing the hydrogen peroxide tolerance of unspecific peroxygenase from Daldinia caldariorum based on structure

Tiantian Li ^a ,
Ruochen Jin ^a ,
Bin Wu ^b ,
Dongming Lan ^a ,
Yunjian Ma ^{a, *,} ,
Yonghua Wang ^{a, c, *,}

a.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China
b.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
c.
Guangdong Youmei Institute of Intelligent Bio-manufacturing Co., Ltd., Foshan 528200, China

myj605740779@scut.edu.cn

yonghw@scut.edu.cn

Received Date: 3 May 2023
Revised Date: 12 June 2023
Accepted Date: 15 June 2023
Available Online: 22 June 2023

Figures(5)

Unspecific peroxygenases (UPOs, EC 1.11.2.1) is a kind of thioheme enzyme capable of catalyzing various oxidations of inert C–H bonds using H₂O₂ as an oxygen donor without cofactors. However, the enhancement of the H₂O₂ tolerance of UPOs is always challenging. In this study, the A161C mutant of rDcaUPO, which originates from Daldinia caldariorum, was found to be highly H₂O₂-resistant. Compared with the wild type, the mutant rDcaUPO-A161C showed a 10-h prolonged half-life and a 64% improved enzyme activity when incubated in 10 mmol/L H₂O₂. The crystal structure analysis at 1.47 Å showed that rDcaUPO-A161C exhibited 10 α-helixes (cyan) and a series of ordered rings, forming a single asymmetric spherical structure. The two conserved domains near heme formed an active site with the catalytic PCP and EHD regions (Glu86, His87, Asp88 residues). The H₂O₂ tolerance of rDcaUPO-A161C was preliminarily explored by comparing its structure with the wild type. Notably, rDcaUPO-A161C showed significantly higher catalytic efficiency than the wild type for the production of hydroxyl fatty acids. This study is anticipated to provide an insight into the structure-function relationship and expand potential applications of UPOs.
- Unspecific peroxygenase,
- Molecular modification,
- Hydrogen peroxide stability,
- Crystal structure,
- Hydroxyl fatty acids
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