Citation: Mingyue Xie, Juan Chen, Yufei Wang, Bojun Liu, Rong-Bin Song, Hong-Min Meng, Zhaohui Li. Ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots[J]. Chinese Chemical Letters, ;2024, 35(2): 108575. doi: 10.1016/j.cclet.2023.108575 shu

Ratiometric fluorescence probe for accurate detection of Concanavalin A by coupling fluorescent microsphere with boric acid functionalized carbon dots

Mingyue Xie ^a ,
Juan Chen ^b ,
Yufei Wang ^a ,
Bojun Liu ^a ,
Rong-Bin Song ^{a, c, *,} ,
Hong-Min Meng ^{a, **,} ,
Zhaohui Li ^{a, **,}

a.
College of Chemistry, Institute of Analytical Chemistry for Life Science, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and their Bioanalytical Applications, Zhengzhou University, Zhengzhou 450001, China
b.
Zhengzhou Key Laboratory of Forensic Science and Technology, Railway Police College, Zhengzhou 450053, China
c.
School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China

^**

rbsong@zzu.edu.cn

hmmeng2017@zzu.edu.cn

zhaohui.li@zzu.edu.cn

Received Date: 19 November 2022
Revised Date: 18 April 2023
Accepted Date: 14 May 2023
Available Online: 19 May 2023

Figures(7)

Accurate and sensitive strategies for Concanavalin A (Con A) sensing are conducive to the better cognition of various important biological and physiological processes. Here, by designing dextran-functionalized fluorescent microspheres (DxFMs) and boric acid-modified carbon dots (BCDs) as recognition unit and built-in signal reference respectively, a ratiometric fluorescent detection platform was proposed for Con A detection with high reliability. In this protocol, the BCDs/DxFMs precipitation was formed due to the covalent interactions between cis-diol of DxFMs and boronic acid groups of BCDs, thus only fluorescence of BCDs could be detected in the supernatant. When Con A was presented, it could bind to DxFMs through its carbohydrate recognition ability and suppress the subsequent assembly between DxFMs and BCDs, leading to the simultaneous capture of DxFMs and BCDs fluorescence in the supernatant. Since the BCDs content was superfluous, their fluorescence intensities were basically constant in all cases. Based on the unchanged BCDs fluorescence signal and target-dependent DxFMs fluorescence signal in supernatant, the ratiometric detection of Con A was realized. Under optimized conditions, this ratiometric fluorescent platform displayed a linear detection range from 0.125 µg/mL to 12.5 µg/mL with a detection limit of 0.089 µg/mL. Moreover, satisfied analytical outcomes for Con A detection in serum samples were obtained, manifesting huge application potential of this ratiometric fluorescent platform in clinical diagnosis.
- Concanavalin A,
- Fluorescent microspheres,
- Carbon dots,
- Ratiometric fluorescent assay,
- Reliability
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