Citation: Jiahao Zhang, Haiyue Peng, Zi'an Chen, Guorui Li, Jing Huang. Photocaged activity-based probes for improved monitoring of protein S-sulfenylation in living cells[J]. Chinese Chemical Letters, ;2024, 35(2): 108560. doi: 10.1016/j.cclet.2023.108560 shu

Photocaged activity-based probes for improved monitoring of protein S-sulfenylation in living cells

State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan Provincial Key Laboratory of Animal Models and Molecular Medicine, School of Biomedical Sciences, Hunan University, Changsha 410082, China

liguorui@hnu.edu.cn

huangjing16@hnu.edu.cn

Received Date: 6 January 2023
Revised Date: 11 April 2023
Accepted Date: 9 May 2023
Available Online: 19 May 2023

Figures(5)

Protein S-sulfenylation (protein sulfenic acid), as one of the most significant oxidative post-translational modifications (OxiPTMs), plays a vital role in regulating protein function. A variety of activity-based probes have been developed to profile sulfenic acid in living cells. However, due to the transient presence and low content of sulfenic acid in living cell, high doses of probes are needed to achieve efficient labeling. More importantly, current probes have no temporal control over sulfenic acid labeling. To overcome these limitations, two caged cysteine sulfenic acid probes DYn-2-ONB and DYn-2-Cou with either an o-nitrobenzyl or coumarin protecting group were developed in this study. Both probes can be efficiently uncaged via irradiation to produce the active C-nucleophile probe DYn-2. Labeling assay in living cells demonstrated DYn-2-ONB exhibited better labeling capacity compared with DYn-2, providing it as a powerful tool for improved monitoring of protein S-sulfenylation in living cells.
- Cysteine,
- Sulfenic acid,
- Activity-based probe,
- Photocage,
- Light activation,
- Protein S-sulfenylation
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沈阳化工大学材料科学与工程学院沈阳 110142