Citation: Zhukang Guo, Baijiang Jin, Yile Fang, Lian Jin, Song Li, Yan Deng, Zhu Chen, Hui Chen, Yuanying Zhang, Rabia Usman, Nongyue He. Automated screening of primary cell-based aptamers for targeting and therapy of pancreatic cancer[J]. Chinese Chemical Letters, ;2024, 35(2): 108528. doi: 10.1016/j.cclet.2023.108528 shu

Automated screening of primary cell-based aptamers for targeting and therapy of pancreatic cancer

Zhukang Guo ^a ,
Baijiang Jin ^a ,
Yile Fang ^a ,
Lian Jin ^b ,
Song Li ^b ,
Yan Deng ^b ,
Zhu Chen ^b ,
Hui Chen ^b ,
Yuanying Zhang ^c ,
Rabia Usman ^a ,
Nongyue He ^{a, b, *,}

a.
State Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
c.
Department of Molecular Biology, Jiangsu Cancer Hospital, Nanjing 210009, China

nyhe1958@163.com

Received Date: 11 February 2023
Revised Date: 26 April 2023
Accepted Date: 27 April 2023
Available Online: 30 April 2023

Figures(4)

Although it has been developed for many years, nucleic acid aptamer screening technology still fails to be widely used, a considerable part of it is due to the variability of tumor cell morphology, which leads to the use of immortalized cell lines in the laboratory to screen nucleic acid aptamers for recognition ability of tumor cells in the diseased body. To address this, primary cells that can be stably passaged were isolated and extracted from spontaneous tumors of genetically engineered pancreatic ductal adenocarcinoma model mice in this study. Next, an automated screening instrument for nucleic acid aptamers developed autonomously by our group was used to perform efficient aptamer screening using a limited number of cells, and the obtained nucleic acid aptamers were affinity verified at the cellular level. Finally, to answer the question of the cell growth environment difference on the recognition ability of nucleic acid aptamers, we verified its targeting ability to tumors in vivo on a nude mice xenograft tumor model, and further used a common antitumor drug doxorubicin combined with nucleic acid aptamers to verify the drug loading ability of this aptamer combined with the targeting therapeutic ability.
- Aptamer,
- Cell-SELEX,
- Automatic instrument,
- Combination therapy,
- Pancreatic cancer
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