Citation: Ying-Ying Chen, Zhu Gui, Di Hu, Meng-Yuan Chen, Jin-Gang He, Si-Yu Yu, Yu-Qi Feng, Jie Wang, Bi-Feng Yuan. Adolescent alcohol exposure changes RNA modifications in adult brain by mass spectrometry-based comprehensive profiling analysis[J]. Chinese Chemical Letters, ;2024, 35(1): 108522. doi: 10.1016/j.cclet.2023.108522 shu

Adolescent alcohol exposure changes RNA modifications in adult brain by mass spectrometry-based comprehensive profiling analysis

Ying-Ying Chen ^{a, b, c, 1} ,
Zhu Gui ^{d, 1} ,
Di Hu ^{e, 1} ,
Meng-Yuan Chen ^a ,
Jin-Gang He ^d ,
Si-Yu Yu ^b ,
Yu-Qi Feng ^a ,
Jie Wang ^{d, f, g, *,} ,
Bi-Feng Yuan ^{a, b, c, *,}

a.
College of Chemistry and Molecular Sciences, Research Center of Public Health, Renmin Hospital of Wuhan University, Wuhan University, Wuhan 430072, China
b.
Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, School of Public Health, Wuhan University, Wuhan 430071, China
c.
Cancer Precision Diagnosis and Treatment and Translational Medicine Hubei Engineering Research Center, Zhongnan Hospital of Wuhan University, Wuhan Research Center for Infectious Diseases and Cancer, Chinese Academy of Medical Sciences, Wuhan 430071, China
d.
Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences-Wuhan National Laboratory for Optoelectronics, Wuhan 430071, China
e.
Phase I Clinical Trial Laboratory, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
f.
Institute of Neuroscience and Brain Diseases, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang 441000, China
g.
University of Chinese Academy of Sciences, Beijing 101408, China

jie.wang@wipm.ac.cn

bfyuan@whu.edu.cn

Received Date: 3 February 2023
Revised Date: 5 April 2023
Accepted Date: 26 April 2023
Available Online: 29 April 2023

Figures(5)

Alcohol consumption is one of the leading causes of death worldwide. Adolescence is a critical period of structural and functional maturation of the brain. Adolescent alcohol use can alter epigenetic modifications. However, little is known on the long-term effects of alcohol consumption during adolescence on RNA epigenetic modifications in brain. Herein, we systematically explored the long-term effects of alcohol exposure during adolescence on small RNA modifications in adult rat brain tissues by comprehensive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis. We totally detected 26 modifications in small RNA of brain tissues. Notably, we observed most of these modifications were decreased in brain tissues. These results suggest that alcohol exposure during adolescence may impose a long-lasting impact on RNA modifications in brain tissues. This is the first report that alcohol use during adolescence can alter RNA modifications in adult brain. Collectively, this study suggests a long-term adverse effects of alcohol consumption on brain from RNA epigenetics angle by comprehensive mass spectrometry analysis.
- Mass spectrometry,
- RNA modification,
- Adolescence,
- Alcohol exposure,
- Brain
1. [1]
  M. Ezzati, E. Riboli, N. Engl. J. Med. 369 (2013) 954–964. doi: 10.1056/NEJMra1203528
2. [2]
  M. Di Nicola, M. Pepe, I. Panaccione, et al., CNS Spectr. 27 (2022) 73–81. doi: 10.1017/s109285292000173x
3. [3]
  M. Roerecke, A. Vafaei, O.S.M. Hasan, et al., Am. J. Gastroenterol. 114 (2019) 1574–1586. doi: 10.14309/ajg.0000000000000340
4. [4]
  S.C. Larsson, S. Burgess, A.M. Mason, et al., Circ. Genom. Precis. Med. 13 (2020) e002814. doi: 10.1161/CIRCGEN.119.002814
5. [5]
  P. Sarich, K. Canfell, S. Egger, et al., Br. J. Cancer 124 (2021) 513–523. doi: 10.1038/s41416-020-01101-2
6. [6]
  L.P. Spear, Nat. Rev. Neurosci. 19 (2018) 197–214. doi: 10.1038/nrn.2018.10
7. [7]
  R. Daviet, G. Aydogan, K. Jagannathan, et al., Nat. Commun. 13 (2022) 1175. doi: 10.1038/s41467-022-28735-5
8. [8]
  Y. Mashhoon, C. Czerkawski, D.J. Crowley, et al., Alcohol. Clin. Exp. Res. 38 (2014) 1955–1964. doi: 10.1111/acer.12475
9. [9]
  M. Luciana, P.F. Collins, R.L. Muetzel, et al., Am. J. Drug Alcohol Abuse 39 (2013) 345–355. doi: 10.3109/00952990.2013.837057
10. [10]
  T.T. Nguyen-Louie, A. Tracas, L.M. Squeglia, et al., Alcohol Clin. Exp. Res. 40 (2016) 1895–1904. doi: 10.1111/acer.13160
11. [11]
  S. Zhao, C.D. Allis, G.G. Wang, Nat. Rev. Cancer 21 (2021) 413–430. doi: 10.1038/s41568-021-00357-x
12. [12]
  S. Moshitch-Moshkovitz, D. Dominissini, G. Rechavi, Cell 185 (2022) 764–776. doi: 10.1016/j.cell.2022.02.007
13. [13]
  Q. Wang, J.H. Ding, J. Xiong, et al., Chin. Chem. Lett. 32 (2021) 3426–3430. doi: 10.1016/j.cclet.2021.05.020
14. [14]
  M. Wang, N.B. Xie, K.K. Chen, et al., Anal. Chem. 95 (2023) 1556–1565.
15. [15]
  J. Xiong, P. Wang, W.X. Shao, et al., Chem. Sci. 13 (2022) 9960–9972. doi: 10.1039/d2sc02446b
16. [16]
  N.B. Xie, M. Wang, T.T. Ji, et al., Chem. Sci. 13 (2022) 7046–7056. doi: 10.1039/d2sc01052f
17. [17]
  Q.F. Zhang, H.M. Xiao, J.T. Zhan, et al., Chin. Chem. Lett. 33 (2022) 4746–4749. doi: 10.1016/j.cclet.2022.01.004
18. [18]
  Y. Feng, N.B. Xie, W.B. Tao, et al., CCS. Chem. 2 (2021) 994–1008. doi: 10.31635/ccschem.020.202000286
19. [19]
  A.J. Sakharkar, E.J. Kyzar, D.P. Gavin, et al., Neuropharmacology 157 (2019) 107679. doi: 10.1016/j.neuropharm.2019.107679
20. [20]
  S. Delaunay, M. Frye, Nat. Cell Biol. 21 (2019) 552–559. doi: 10.1038/s41556-019-0319-0
21. [21]
  P. Boccaletto, F. Stefaniak, A. Ray, et al., Nucleic Acids Res. 50 (2022) D231–D235. doi: 10.1093/nar/gkab1083
22. [22]
  X.M. Tang, T.T. Ye, X.J. You, et al., Chin. Chem. Lett. 34 (2023) 107531. doi: 10.1016/j.cclet.2022.05.045
23. [23]
  Y.J. Feng, X.J. You, J.H. Ding, et al., Anal. Chem. 94 (2022) 4747–4755. doi: 10.1021/acs.analchem.1c05292
24. [24]
  Y. Hu, X. Hong, Z. Yuan, et al., Chin. Chem. Lett. 34 (2023) 108023. doi: 10.1016/j.cclet.2022.108023
25. [25]
  J. Li, W.Y. Zhu, W.Q. Yang, et al., Sci. China Life Sci. 64 (2021) 1423–1436. doi: 10.1007/s11427-020-1906-4
26. [26]
  M.Y. Chen, C.B. Qi, X.M. Tang, et al., Chin. Chem. Lett. 33 (2022) 3772–3776. doi: 10.1016/j.cclet.2021.12.008
27. [27]
  M.D. Lan, J. Xiong, X.J. You, et al., Chem. Eur. J. 24 (2018) 9949–9956. doi: 10.1002/chem.201801640
28. [28]
  D. Wiener, S. Schwartz, Nat. Rev. Genet. 22 (2021) 119–131. doi: 10.1038/s41576-020-00295-8
29. [29]
  I. Barbieri, T. Kouzarides, Nat. Rev. Cancer 20 (2020) 303–322. doi: 10.1038/s41568-020-0253-2
30. [30]
  T. Suzuki, Nat Rev Mol. Cell. Biol. 22 (2021) 375–392. doi: 10.1038/s41580-021-00342-0
31. [31]
  M.Y. Chen, Z. Gui, K.K. Chen, et al., Chin. Chem. Lett. 33 (2022) 2086–2090. doi: 10.1016/j.cclet.2021.08.094
32. [32]
  M.Y. Cheng, X.J. You, J.H. Ding, et al., Chem. Sci. 12 (2021) 8149–8156. doi: 10.1039/d1sc01972d
33. [33]
  X.J. You, S. Zhang, J.J. Chen, et al., Nucleic Acids Res. 50 (2022) 9858–9872. doi: 10.1093/nar/gkac770
34. [34]
  D. Su, C.T. Chan, C. Gu, et al., Nat. Protoc. 9 (2014) 828–841. doi: 10.1038/nprot.2014.047
35. [35]
  J.W. Yarham, T.N. Lamichhane, A. Pyle, et al., PLoS Genet. 10 (2014) e1004424. doi: 10.1371/journal.pgen.1004424
36. [36]
  S. Murakami, S.R. Jaffrey, Mol. Cell 82 (2022) 2236–2251. doi: 10.1016/j.molcel.2022.05.029
37. [37]
  A.M. Shafik, F. Zhang, Z. Guo, et al., Genome Biol. 22 (2021) 17. doi: 10.1186/s13059-020-02249-z
38. [38]
  J. Widagdo, Q.Y. Zhao, M.J. Kempen, et al., J. Neurosci. 36 (2016) 6771–6777. doi: 10.1523/JNEUROSCI.4053-15.2016
39. [39]
  X.J. You, L. Li, T.T. Ji, et al., Chin. Chem. Lett. 34 (2023) 107181. doi: 10.1016/j.cclet.2022.01.074
40. [40]
  O. Gabay, Y. Shoshan, E. Kopel, et al., Nat. Commun. 13 (2022) 1184. doi: 10.1038/s41467-022-28841-4
41. [41]
  J.H. Ding, M.Y. Chen, N.B. Xie, et al., Biosens. Bioelectron. 219 (2023) 114821. doi: 10.1016/j.bios.2022.114821
42. [42]
  J.J. Chen, X.J. You, L. Li, et al., Anal. Chem. 94 (2022) 8740–8747. doi: 10.1021/acs.analchem.2c01226
43. [43]
  S.S. Tran, H.I. Jun, J.H. Bahn, et al., Nat. Neurosci. 22 (2019) 25–36. doi: 10.1038/s41593-018-0287-x
1. [1]
  Tian Feng , Yun-Ling Gao , Di Hu , Ke-Yu Yuan , Shu-Yi Gu , Yao-Hua Gu , Si-Yu Yu , Jun Xiong , Yu-Qi Feng , Jie Wang , Bi-Feng Yuan . Chronic sleep deprivation induces alterations in DNA and RNA modifications by liquid chromatography-mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(8): 109259-. doi: 10.1016/j.cclet.2023.109259
2. [2]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
3. [3]
  Jun Xiong , Ke-Ke Chen , Neng-Bin Xie , Wei Chen , Wen-Xuan Shao , Tong-Tong Ji , Si-Yu Yu , Yu-Qi Feng , Bi-Feng Yuan . Demethylase-assisted site-specific detection of N¹-methyladenosine in RNA. Chinese Chemical Letters, 2024, 35(5): 108953-. doi: 10.1016/j.cclet.2023.108953
4. [4]
  Qiongqiong Wan , Yanan Xiao , Guifang Feng , Xin Dong , Wenjing Nie , Ming Gao , Qingtao Meng , Suming Chen . Visible-light-activated aziridination reaction enables simultaneous resolving of C=C bond location and the sn-position isomers in lipids. Chinese Chemical Letters, 2024, 35(4): 108775-. doi: 10.1016/j.cclet.2023.108775
5. [5]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
6. [6]
  Zongyi Huang , Cheng Guo , Quanxing Zheng , Hongliang Lu , Pengfei Ma , Zhengzhong Fang , Pengfei Sun , Xiaodong Yi , Zhou Chen . Efficient photocatalytic biomass-alcohol conversion with simultaneous hydrogen evolution over ultrathin 2D NiS/Ni-CdS photocatalyst. Chinese Chemical Letters, 2024, 35(7): 109580-. doi: 10.1016/j.cclet.2024.109580
7. [7]
  Jing Chen , Peisi Xie , Pengfei Wu , Yu He , Zian Lin , Zongwei Cai . MALDI coupled with laser-postionization and trapped ion mobility spectrometry contribute to the enhanced detection of lipids in cancer cell spheroids. Chinese Chemical Letters, 2024, 35(4): 108895-. doi: 10.1016/j.cclet.2023.108895
8. [8]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
9. [9]
  Kexin Yuan , Yulei Liu , Haoran Feng , Yi Liu , Jun Cheng , Beiyang Luo , Qinglian Wu , Xinyu Zhang , Ying Wang , Xian Bao , Wanqian Guo , Jun Ma . Unlocking the potential of thin-film composite reverse osmosis membrane performance: Insights from mass transfer modeling. Chinese Chemical Letters, 2024, 35(5): 109022-. doi: 10.1016/j.cclet.2023.109022
10. [10]
  Haoyang Wang , Ronghao Zhang , Yanlun Ren , Li Zhang . A convenient method for measuring gas-liquid volumetric mass transfer coefficient in micro reactors. Chinese Chemical Letters, 2024, 35(4): 108833-. doi: 10.1016/j.cclet.2023.108833
11. [11]
  Wenbi Wu , Yinchu Dong , Haofan Liu , Xuebing Jiang , Li Li , Yi Zhang , Maling Gou . Modification of plasma protein for bioprinting via photopolymerization. Chinese Chemical Letters, 2024, 35(8): 109260-. doi: 10.1016/j.cclet.2023.109260
12. [12]
  Lijun Mao , Shuo Li , Xin Zhang , Zhan-Ting Li , Da Ma . Cucurbit[n]uril-based nanostructure construction and modification. Chinese Chemical Letters, 2024, 35(8): 109363-. doi: 10.1016/j.cclet.2023.109363
13. [13]
  Fangling Cui , Zongjie Hu , Jiayu Huang , Xiaoju Li , Ruihu Wang . MXene-based materials for separator modification of lithium-sulfur batteries. Chinese Journal of Structural Chemistry, 2024, 43(7): 100337-100337. doi: 10.1016/j.cjsc.2024.100337
14. [14]
  Chenghao Ge , Peng Wang , Pei Yuan , Tai Wu , Rongjun Zhao , Rong Huang , Lin Xie , Yong Hua . Tuning hot carrier transfer dynamics by perovskite surface modification. Chinese Chemical Letters, 2024, 35(10): 109352-. doi: 10.1016/j.cclet.2023.109352
15. [15]
  Yue Wang , Caixia Xu , Xingtao Tian , Siyu Wang , Yan Zhao . Challenges and Modification Strategies of High-Voltage Cathode Materials for Li-ion Batteries. Chinese Journal of Structural Chemistry, 2023, 42(10): 100167-100167. doi: 10.1016/j.cjsc.2023.100167
16. [16]
  Shu-Ran Xu , Fang-Xing Xiao . Metal halide perovskites quantum dots: Synthesis, and modification strategies for solar CO2 conversion. Chinese Journal of Structural Chemistry, 2023, 42(12): 100173-100173. doi: 10.1016/j.cjsc.2023.100173
17. [17]
  Fengyu Zhang , Yali Liang , Zhangran Ye , Lei Deng , Yunna Guo , Ping Qiu , Peng Jia , Qiaobao Zhang , Liqiang Zhang . Enhanced electrochemical performance of nanoscale single crystal NMC811 modification by coating LiNbO₃. Chinese Chemical Letters, 2024, 35(5): 108655-. doi: 10.1016/j.cclet.2023.108655
18. [18]
  Huan Yao , Jian Qin , Yan-Fang Wang , Song-Meng Wang , Liu-Huan Yi , Shi-Yao Li , Fangfang Du , Liu-Pan Yang , Li-Li Wang . Ultra-highly selective recognition of nucleosides over nucleotides by rational modification of tetralactam macrocycle and its application in enzyme assay. Chinese Chemical Letters, 2024, 35(6): 109154-. doi: 10.1016/j.cclet.2023.109154
19. [19]
  Xin Zhang , Junyu Chen , Xiang Pei , Linxin Yang , Liang Wang , Luona Chen , Guangmei Yang , Xibo Pei , Qianbing Wan , Jian Wang . Drug-loading ZIF-8 for modification of microporous bone scaffold to promote vascularized bone regeneration. Chinese Chemical Letters, 2024, 35(6): 108889-. doi: 10.1016/j.cclet.2023.108889
20. [20]
  Zeyu XU , Tongzhou LU , Haibo SHAO , Jianming WANG . Preparation and electrochemical lithium storage performance of porous silicon microsphere composite with metal modification and carbon coating. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1995-2008. doi: 10.11862/CJIC.20240164

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(193)
HTML views(17)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142